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Treat each flask as follows. Add 5.0 mL of a solution prepared by dissolving 14 g of boron trifluoride in methanol to make 100 mL, swirl to mix, and reflux for 15 minutes or until the solid is dissolved.

在每一个锥形瓶中，加5.0mL三氟化硼溶液（取14g三氟化硼，用甲醇溶解并稀释至100ml），搅拌使混合，回流15分钟或直至固体物溶解。

TNF-α and IL-10 plasma concentrations were measured by ELISA. Results: Plasma concentrations of TNF-α fell significantly from (419.4±77.5) pg/ml to (168.1±55.4) pg/ml after treatment, much lower than that of MODS group (P.05), but we did not find TNF-α in the ultrafiltrate. The start of HVHF was associated with a significant and sustained fall of IL-10, which came to the lowest level in the 6th hour after new filter was used, thereafter remained stable during the observation period.

姑果：HVHF组治疗后血浆TNF-α浓度明显下降，从(419.4±77.5)pg/ml降低到(168.1±55.4)pg/ml，各时间点与MODS组有显著差异(P.05)，但滤液中未检测到TNF-α治疗开始后血浆IL-10明显下降，6h达到最低点，此后维持在稳定状态，滤液中能检测到IL-10，随治疗时间延长其滤过系数降低。

The8 transgenic mice lactated naturally .and centrifuge to discard the fatness and the acid. At last ,we got the serum of the milk of 5 transgenic mice and 2 normal mice , checked the human insulin in serum by RlA.The result of the CPM show that two transgenic mice are conspicuous excess to the other transgenic mice and normal mice .and the mount of human insulin achieve respectively to 250uIU/ml ,376 ulU/ml.

转基因小鼠自然泌乳以后，收集到5只转基因小鼠和2只阴性小鼠的乳汁，脱脂脱酸以后，在乳清中用放射免疫检测方法检测胰岛素的表达量，结果其中两只转基因小鼠的表达量明显高于其它小鼠，表达量达到250uIU／ml和376uIU／ml，进一步的蛋白质定性和活性检测正在继续进行中。

Result The approach was used in 11 patients with malignant tumors of liver from June 1994 to January 1996. Operation time was 192 min (150～250 min) and estimated blood loss during operation was 1460 ml (800～4200 ml). No operative mortality was found and all of the patients recovered uneventfully.

结果 1994年6月至1996年1月已用该法切除肝脏恶性肿瘤11例，平均手术时间为192 min（150～250 min），术中平均失血量1460 ml（800～4200 ml），全组无手术死亡，术后均顺利恢复。

Results Two months after transplantation, there are significant differences in secreting function of microencapsulated parathyroid tissue between purified and unpurified groups(P＜0.05),(38.8±13.7) pg/ml and (1.2±0.7) pg/ml respectively. The retrieve rates of purified and unpurified alginate empty microcapsules are （72±18）%and 24±13）%,（65±21）%and 0 respectively after 1 and 2 months of transplantation.

结果移植后2个月纯化组及未纯化组人甲状旁腺组织分泌功能有显著差异（P.05），分别为(38.8±13.7) pg/ml和(1.2±0.7) pg/ml，纯化及未纯化海藻酸钠制备空囊移植组，植入1个月时微囊冲洗回收率分别为（72±18）%和（24±13）%，植入2个月时为（65±21）%和0。

N L-Leucine Producer,Strain No .145,was obtained from mutants resistant to α-amino-β-hydroxyl valeric acid and rifampin by mutagenizing Brevibacterium Tianjinese T6-13 with ul-traviolet rays,It produced L-Leucine up to 32mg/mL on shaker and 21.8mg/mL of L-Leucine was produced in 16 liter fermentor in 60 hours.

以天津短杆菌T6～13为出发菌株，采用紫外线进行诱变处理，筛选α－氨基－β－羟基戊酸和利福平抗性突变株，获得一株L－亮氨酸产生菌No.145，L－亮氨酸摇瓶产量可以达32.1mg／mL。在16L发酵罐中培养60h，L－亮氨酸产量可以达21.8mg／mL葡萄糖转化率可以达20％。

After treated the spinal cord slice at E18 ~ P0 with ChABC at a final concentration of 0.1 U/ml, CGRP-LI fibers in the superficial layers of DH and their branches were seen to extend ventrally instead. When the concentration of ChABC increased to 0.5 U/ml, the above changes were more obvious.

2用0.1 U/ml终浓度的ChABC处理E18 ~ P0的小鼠脊髓片后，原本由DH浅层外侧部向腹内侧延伸的CGRP-LI轴突转为向腹外侧延伸，由DH浅层内侧部向腹外侧延伸的轴突同原本向背侧延伸的分支一起变为向腹侧生长。

To the separatory funnel in 10 ml acetate buffer and dispensable 1 ml of sodium thiosulfate solution, blending, followed by a second all-Canadian 10.0mL tetrachlorocarbon dithizone-0.01g/L solution, vibrating 2min, standing out carbon tetrachloride layer to 1 cm chromatometry Cup, zero-zero, adjusting to 520nm wavelength measuring the absorbance at, draw a comparison between the quantitative standard curve.

向各分液漏斗内加5mL乙酸盐缓冲液及1mL硫代硫酸钠溶液，混匀后再各加10.0mL二硫腙－四氯化碳溶液(0.01g/L)，振摇2min，静置分层，分出四氯化碳层于1cm比色杯中，以零管调节零点，于520nm波长处测吸光度，绘制标准曲线比较定量。

Their 95% effective concentration (EC95) value was 0.4735 μg/mL. Flumorph at the concentration of 0.5 μg/mL, can lead the inhibit rate of growth of mycelium, formulation of sporangium and germination of zoospore of Phytophthora sojae to 100%, the concentration was lower than Apron XL, Metalaxy which lead to the same inhibition rate. It indicated that Flumorph can obviously inhibit the growth of P.sojae in vitro, and effect is better than that of Apron XL and Metalaxy.

结果表明：氟吗啉对所测定的菌株4个发育阶段均有显著的抑制作用，氟吗啉对大豆疫霉菌菌丝生长EC50 值为0.1906μg/mL，EC95 值为0.4735 μg/mL；在0.5 μg/mL 浓度下对大豆疫霉菌孢子囊形成和孢子萌发抑制率达100%，低于达到相同抑制效果的金阿普隆和甲霜灵；说明该药剂在离体条件下能够有效抑制大豆疫霉菌的生长，效果好于金阿普隆和甲霜灵。

32 U/mL enzyme activity can be detected in medium after fermentation. By optimizing the zymotic medium and fermentation conditions, recombination B. subtilis can express as high as 110.02 U/mL of Alkalineβ-mannanase. Cycle of fermentation needs 5 days(4 days is needed in induced expression ), then enzyme activity maintained at the highest level, less than 5 % enzyme activity was lost in next 2 days.

为了继续对碱性β-甘露聚糖酶进行高表达研究，试验采用了芽孢杆菌发酵培养基来进行产酶研究，初步诱导酶活达到29.32 U/mL，通过对发酵条件的优化，该重组枯草芽孢杆菌的产酶水平有了大幅度的提高，最高可达110.02 U/mL。

Based on the relationship and the phenomenon model, an improved model of MR damper has been presented in this paper.

摘 要：本文在实验数据的基础上建立了磁流变阻尼器输出力与不同常电流控制量的关系，对现有的 p模型进行了改进。

I don't think our founding fathers were very fuckable.

我认为我们的开国元老没什么吸引力