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Micronucleus test results exhibited that significant differences in micronuclear rates were determined between 0.4 mL bone cement and 0.4 mL microsphere-coated bone cement groups and negative control group, but no significant difference was detected among other groups. This revealed that this microsphere-coated bone cement leaching liquor did not show significant cell heredity toxic effects. Nevertheless, microsphere-coated bone cement in the high concentration and high dosage groups exhibited low toxic effects, mainly showing increased hemolysis rate and micronuclear rate. Thus, it was necessary to control the dosage and concentration of microsphere-coated bone cement.

微核实验结果显示除0.4 mL骨水泥组和0.4 mL微球骨水泥组的微核率与阴性对照组差异有显著性意义，其余各组差异无显著性意义，说明该微球骨水泥的浸提液无明显细胞遗传毒性作用，但是高浓度和高剂量组的微球骨水泥仍有较低的毒性作用，主要表现为溶血率和微核率的提高，因此在应用时要适当控制微球骨水泥的剂量和浓度。

When the concentration of L-phenylalanine was 6 g/L,the optimum values of volume ratio of organic phase to aqueous phase,reaction temperature,pH value and liquid volume were 1∶1,30 ℃,4.0 and 20 mL/250 mL respectively. Under above optimum conditions,the content of 2-phenylethylalcohol reached 3.0 g/L 48 h later,which increased by about 50% than that in monophasic aqueous system.

当初始底物浓度为6 g/L、水和油酸的相体积比为1∶1、反应温度30 ℃和pH值4.0、装液量20 mL/250 mL 的条件下，转化48 h，产物浓度达到3.0 g/L，比单水相提高了50%。

METHODS: Fetal liver was obtained sterilely. Monoplast suspension was collected by collagenase digestion and mechanical separation, and then centrifuged using 1.070 g/mL Percoll separating medium and 1.077 g/mL Ficoll separating medium. Cells achieved from the interface of separatory liquids were cultured in DMEM/F12 medium, supplemented with 0.1 volume fraction of fetal bovine serum, 20 μg/L hepatocyte growth factor and 40 μg/L epidermal growth factor, for 9 days.

无菌状态下取出胎儿肝脏，以胶原酶消化法和机械分离法获得胎儿肝脏来源单细胞悬液，分别采用1.070 g/mL Percoll分离液和1.077 g/mL Ficoll分离液进行密度梯度离心，吸取界层细胞，添加含体积分数为0.1 FBS、20 μg/L肝细胞生长因子、40 μg/L表皮生长因子的DMEM/F12新鲜培养基诱导9 d。

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

The results indicated that ethanolic extract of Andrographis paniculata and Morinda citrifolia at concentrations 1.25 μg/mL were superior to standards of Trolox and rutin 1.25 μg/mL appeared to have a lengthening in lag phase.

结果显示1.25 μg/mL穿心莲乙醇萃取物和海巴戟天叶乙醇萃取物比标准品Trolox 和 rutin 1.25 μg/mL 更能延长迟滞期。

After catheter insertion under transoral endoscopic guidance, animals were randomly assigned to one of the four groups: acid reflux (pepsin 0.3 mg/mL + taurodeoxycholic acid 0.155 mg/mL + acid pH 2); nonacid reflux (pepsin + taurodeoxycholic acid + pH 6); saline reflux; and unwounded control.

经口腔内镜引导下插入导管，并随机将兔子分组：酸返流组（胃蛋白酶0.3mg/ml＋牛磺脱氧胆酸0.155mg/ml＋酸pH＝2）；非酸返流组（胃蛋白酶＋牛磺脱氧胆酸＋pH＝6）；生理盐返流组；以及无损伤组。

When induced to differentiate in the presence of Oleaceae oil (35-130 mg/ml), 3T3-L1 preadipocytes dose- dependently reduced their ability to accumulate cellular triglycerides. Cytoplasmic triglyceride droplets could also be visualized by microscopic observation. Cells treated with 130 mg/ml Oleaceae oil retained their morphology as spindly fibroblasts, while normal adipocytes take on a rounded shape filled with triglyceride droplets.

在开始刺激细胞分化的同时，以 35 mg/ml 到 130 mg/ml 不同的木樨科精油浓度处理细胞，为期八天，将分化到第八天的细胞收下来测三酸甘油酯的浓度当作分化程度的指标，结果发现随著木樨科精油浓度的增加，细胞所累积的三酸甘油酯浓度就越低，用显微镜观察即可发现细胞中脂肪油滴所占的比例明显降低。

The results indicated that the bioactivity(touch kill, oviposit inhibition, repellency)of methyl linoleate was high , the adjusted mortalities of female mite and egg were 82.82% and 78.54%, Oviposit inhibit ion rate and repellency rate were 78.46% and 95.61%,respectively at the concentration of 5 mg/ml.

亚油酸甲酯具有较强的触杀、产卵抑制和驱避作用，浓度为5 mg/ml时，对朱砂叶螨的雌成螨和卵24h后的校正死亡率分别达到82.82%和78.54%，LC50分别为1.4434mg/ml和2.0283mg/ml；对雌成螨24h后的产卵抑制率和驱避率分别达到78.46%和95.61%。

From Chuxiong Shuangbai was the highest metalaxyl resistant isolate,and the lowest resistance isolate 218# come from Weishanof Dali. The EC50 of 34 Phytophthora parasitica var. nicotianae isolates against metalaxyl was 0.0148μg/ml in average, was in the range of 0~0.053μg/ml.

分离自楚雄双柏县雨龙乡的113#菌株抗药性最强，来自大理巍山县大仓镇的218#菌株抗药性最低。34株烟草黑胫病菌菌株对甲霜灵的EC50值平均为0.0148μg/ml，范围在0~0.053μg/ml。

The result indicated that: the solubility of phosphorus pentafluoride in aether was decreasing as temperature rising; when temperature rises from 0℃ to 30℃, the solubility of phosphorus pentafluoride in aether decrease from 1.5448g/mL to 0.7335g/mL.

通过实验发现，五氟化磷在乙醚中的溶解度随温度的升高而下降，当温度从0℃上升至30℃时，五氟化磷在乙醚中的溶解度从1.5448g/mL下降到0.7335g/mL。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。