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32 U/mL enzyme activity can be detected in medium after fermentation. By optimizing the zymotic medium and fermentation conditions, recombination B. subtilis can express as high as 110.02 U/mL of Alkalineβ-mannanase. Cycle of fermentation needs 5 days(4 days is needed in induced expression ), then enzyme activity maintained at the highest level, less than 5 % enzyme activity was lost in next 2 days.

为了继续对碱性β-甘露聚糖酶进行高表达研究，试验采用了芽孢杆菌发酵培养基来进行产酶研究，初步诱导酶活达到29.32 U/mL，通过对发酵条件的优化，该重组枯草芽孢杆菌的产酶水平有了大幅度的提高，最高可达110.02 U/mL。

objective to determine the contents of residual acetone in acetoacetic ester,the raw material for the synthesis of edaravone, by gas chromatography.methods sample solution was injected on a gdx column (0.18 mm～0.25 mm) with nitrogen as the carrier gas.the residue was calculated by the external standard method.results the linearity was good over the range of 25 μg/ml～400 μg/ml,with the correlation coefficient of acetone being 0.996 and the average recovery 96.8%.conclusions the method was proved to be simple,rapid,practical and highly sensitive.it is an efficient method for the quality control of acetoacetic ester.

摘要］ 目的：用气相色谱法测定依达拉奉合成起始原料乙酰乙酸乙酯中丙酮的残留量。方法：采用固定相为二乙烯苯乙基乙烯苯型高分子多孔小球（直径为0.18 mm～0.25 mm），氮气为载气，用外标法测定乙酰乙酸乙酯中残留丙酮的含量。结果：该方法在丙酮浓度范围在25 μg/ml～400 μg/ml内呈良好的线性关系，响应曲线相关系数为0.996，平均回收率为96.8%。结论：本法简便，快速、实用，灵敏度高，可有效地用于乙酰乙酸乙酯的质量控制。

The BMSCs were divided into six groups after repeatedly passaged: A,the BMSCs were cultured with conventional culture fluid(DMEM culture fluid+20%fetal bovine serum+2 mmol/L aminoglutaric acid amine) all the time;B,the BMSCs were cultured with conventional culture fluid+HGF(25ng/ml)+dexamethasone10~(-7M;C(HGF and Zuoguiwan induced group), the BMSCs were cultured with conventional culture fluid+ HGF(25ng/ml)+ dexamethasone10~(-7M+ 10%Zuoguiwan drug serum;D(conditioned medium and contrast serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % normal rat serum;E(conditioned medium and Bazhentang drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % Bazhentang drug serum;F(conditioned medium and Zuoguiwan drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10% Zuoguiwan drug serum.

常规培养组始终使用常规培养液(DMEM培养液+体积分数20％胎牛血清+2mmol／L谷氨酸胺)进行培养；HGF诱导组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7M进行培养；HGF加左归丸组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7／M+10％的左归丸含药血清进行培养；条件培养液加对照血清组以常规培养液+50％的条件培养液+10％正常大鼠血清进行培养；条件培养液加八珍汤组以常规培养液+50％的条件培养液+10％八珍汤含药血清进行培养；条件培养液加左归丸组以常规培养液+50％的条件培养液+10％左归丸含药血清进行培养。

There were no concentrations less than 5 mcg/mL; Average concentration of all pre-hemodialysis was 12.6 ± 3.6 mcg/mL. Average concentration of all pre-hemodialysis for anuric patients (n=10) was 14.2±3.6 mcg/mL.

结果：17位病患，总共获得102个药物浓度，全部透析前浓度介於5~20有49 (96.1%)个浓度，大於20有2 (3.9%)个浓度，最低浓度为7.22，最高为22.75，没有低於5的浓度；全部血液透析前浓度的平均值为12.6±3.6；没有残余肾功能的患者(n=10)平均的血中浓度为14.2±3.6 mcg/mL。

METHODS:We recorded the cardiogram by perfusing garlicin injection and garlic preparation to the isolated heart of rabbits by Langendorff method,examined the minimum dose of asystole. RESULTS:The minimum fungistatic concentration of garlicin injection was 3～6.2 times lower than that of garlic preparation. The minimum dose of garlic preparation is 0.1736 u/ml, and that of garlicin injection is 3.919 u/ml.

采用Langendorff法对兔离体心脏分别灌流大蒜素注射液、大蒜注射液、大蒜实验液，分别记录心搏图，求出致心脏停搏的最小剂量，结果：大蒜素注射液的抑菌浓度为大蒜实验液的1/6.2～1/3，而致心脏停搏的最小浓度分别为0.1736 u/ml和3.919 u/ml。

Methods Ovarian GC DNA fragments of both atretic and developing follicles before and after estrogen (1 μg/ml), androgen (1 μg/ml) treatments were analysed by agarose gel electrophoresis. The expression of bcl-2 mRNA was also determined by northern blotting in GC of developing follicle after sex steroids addition. Results Internucleosomal DNA cleavage occurred in granulosa cells in atretic follicles and after androgen treatment.

应用细胞培养、选择性DNA抽提和琼脂糖凝胶电泳技术分析闭锁卵泡和发育中卵泡颗粒细胞的凋亡状况，及雌激素(1 μg/ml)、雄激素(1 μg/ml)对体外培养发育中的卵泡颗粒细胞凋亡的作用；用Northern印迹杂交技术检测经雌、雄激素刺激后发育中的卵泡颗粒细胞bcl-2基因mRNA表达的变化。

Color reaction was performed in organic solution-salt solution biphasic system. The organic solution was 1,2-Dichloroethane-Isoamyl alcohol mixture (V/V=96:4) added Bromophenol blue (per 100 mL added 0.05 g). The salt solution was 55%K2HPO4 aqueous solution added Na3CO3 (per 100 mL added 14 g), under the temperature of color reaction between 12℃ to 25℃.

显色反应在有机溶液－盐溶液两相体系中进行，有机溶液为每100 mL 1，2-二氯乙烷－异戊醇混合溶液（体积比96：4）加入0.05 g溴酚蓝，12~25℃显色反应条件下，盐溶液为每100 mL 55%K2 HPO4水溶液加入14 g Na2CO3。

Patients with anaphylactic shock unresponsive to fluid challenge (especially if accompanied by bronchoconstriction) receive epinephrine 0.05 to 0.1 mg IV, followed by epinephrine infusion of 5 mg in 500 mL 5% D/W at 10 mL/h or 0.02 μg/kg/min.

输液无效的过敏性休克病人（尤其是伴有支气管收缩者）可先静脉给注肾上腺素0.05至0.1 mg，然后再用5 mg 肾上腺素加500 mL 5%葡萄糖液滴注，滴速10 mL/h or 0.02 μg/kg/min。

The metabolites were mainly aldehydes and acids when the level of inoculation was 10~9cfu/ml, however, alcohols, acids and esters were produced when the level of inoculation was 10~8cfu/ml. The optimal temperature and pH for production of 3-methyl butanal were 40℃ and 7.0, but NaCl and NaNO_2 inhibited its production.

接种量不同会导致代谢BCAA的产物不同：接种量为10~9 cfu/ml时生成相应的醛和酸；接种量为10~8 cfu/ml时生成相应的醇、酸和酯。S52菌株在40℃，pH 7.0，无亚硝酸盐和NaCl的情况下产3-甲基丁醛的量最多。

For ferric nanoparticles, at the optimum condition of pH7.4 sodium cacodylate buffer solution, the detection limit of bovine serum albumin is 6.6ng/mL and the linear range locate in 0.02-0.70ug/mL.

纳米铁在最佳试验条件下，即pH7.4的二甲砷酸钠缓沖介质中，BSA测定的检测限为6.6ng/mL，线性范围为0.02～0.70μg/mL。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。