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Transfer 50 ml of this solution to 200-ml Erlenmeyer flask, or, if smaller aliquot is taken, dilute to about 50 ml with water. Add 10 ml 4 N H2SO4, 1 ml KI,1 drop ammonium molybdate solution, and 50 ml water. With continuous agitation, add excess of 0.00359 N Na2S2O3 (5 10 ml).

移取50毫升上述溶液于200毫升于锥形瓶中，如果取样量较少，用水稀释到50 毫升，加入10 ml 4 N H2SO4，1 ml KI， 1滴钼酸胺溶液和50毫升水，边搅拌边加入过量的0.00359 N Na2S2O3溶液(5 10 ml)。

The results indicated that HDPCs'proliferation was inhibited significantly when they were cultured in DMEM with 50、100μg/mlLPS. Low-dose of Chinese nutgall water extracts( 1.25、2.5、5μg/ml) resisted the inhibitory effect of LPS to HDPCs'proliferation significantly .From the third day , the proliferation activity of HDPCs incubated with 2.5μg/ml Chinese nutgall water extract and LPS increased signicificantly in a time-dependent manner.

结果显示，培养液中加入浓度为50μg/ml、100μg/ml的LPS时，HDPCs的增殖活性受到明显抑制，加入低浓度(1.25μg/ml、2.5μg/ml、5μg/ml)五倍子水提取物能明显拮抗100μg/ml的LPS对HDPCs增殖的抑制作用，2.5μg/ml五倍子水提取物拮抗100μg/ml的LPS对HDPCs增殖抑制作用第3天开始明显增加，并呈时间依赖性。

The results were as follows: the activity of prawns was highest under the condition of pH 7.0, 35 ℃ using pyrocatechol as substrate; four inhibitors i.e ascorbic acid1 mg·mL~(-1, sodium sulphite2 mg·mL~(-1, phytic acid1 mL·L~(-1 and EDTA0.1 mg·mL~(-1 had some inhibiting effects on the PPO of prawns, in which the effect of phytic acid1 mL·L~(-1 was the best; the PPO activity of prawns increased gradurally during the storage period; the PPO activity of prawns was inhibited in vacuum environment, the lower the storing temperature was, the stronger the PPO activity was.

结果表明：以邻苯二酚为底物，虾PPO活性的最适pH为7。 0，最适温度为35 ℃；4种抑制剂(1 mg·mL-1抗坏血酸、2 mg·mL-1亚硫酸钠、1 mL·L-1植酸及0。 1 mg·mL-1EDTA)对南美白对虾PPO都表现出一定的抑制作用，以1 mL·L-1植酸抑制作用最强；南美白对虾在贮藏过程中PPO活性逐渐上升，真空环境中PPO活性受到抑制，贮藏温度越低，抑制作用越强。

Results showed that in the water body of Xizi Lake, annual average of culturable planktonic ammonifiers and nitrogen fixers were 510 and 236 CFU/mL, respectively; Ammonia oxidizers, nitrite oxidizers, nitrate reducers and denitrifiers were 8.5, 16, 587 and 16 MPN/mL, respectively; Inorganic phosphate solubilizing bacteria (1PB) and organic phosphorus mineralizing bacteria were 89 CFU/mL and 37 MPN/mL, aerobic and anaerobic cellulose decomposers were 7 and 5 MPN/mL, respectively.

水体中可培养异养细菌和固氮菌的年平均值分别为510和236CFU／mL，氨氧化细菌、亚硝酸氧化细菌、硝酸盐还原菌和脱氮菌的数量分别为8.5、16、587和16 MPN／mL；无机磷和有机磷分解菌分别为89 CFU／mL和37 MPN／mL；好氧性纤维素分解菌和厌氧性纤维素分解菌只有7和5 MPN／mL。

The fungicidal activity of methanol extract was higher than that of ethyl acetate extract, and the inhibition rates were higher than 90% against Alternaria longipes, Fusarium oxysporium, Gibberella zeae, Glomerella cingulata, Exserohilum turcicum under the concentration of 1000g/mL The results of the inhibition of spore germination indicated that the fermentation products exhibited obvious inhibition rate against Alternaria longipes, Exserohilum turcicum and Fusarium coeruleum, the EC50 values was 59.4g/mL, 96.5g/mL and 132.5g/mL. In the bioassay of the fermentation products against Bacillus sub tills and Bacillus cereus, by means of detection fo inhibition zone, the inhibition zone diameter was 20mm and 27mm. The results of pot tests that methanol extract showed higher than 70% of protective efficacy and therapeutic efficacy against wheat powdery mildew under the concentration of 2000g/mL.

其中，甲醇提取物的抑菌活性比乙酸乙酯提取物强，在1000g/mL浓度下对烟草赤星病菌、马铃薯干腐病菌、小麦赤霉病菌、苹果炭疽病菌、玉米大斑病菌的菌丝生长抑制率均大于90%；抑制孢子萌发法试验结果表明，2K3菌株菌丝体的甲醇提取物对烟草赤星病菌玉米大斑病菌和马铃薯干腐病菌的EC50分别为59.4g/mL、96.5g/mL和132.5g/mL；管碟法生测结果表明，2K3菌株菌丝体的甲醇提取物对枯草芽孢杆菌和蜡状芽孢杆菌的抑菌圈直径分别为20mm和27mm；盆栽试验结果表明，2K3菌株菌丝体的甲醇提取物在2000g/mL浓度下对小麦白粉病的治疗和保护作用均在70%以上。

Rats in the experimental group received conventional fixation, and then decalcified with modified decalcifying fluid [10 mL of methanoic acid, 8 mL of concentrated hydrochloric acid (1.19 relative density, 3 g of aluminum chloride, 20 mL of formaldehyde (0.4 volume fraction), 3 mL of glacial acetic acid, adding to 200 mL with saline solution], dehydrated, transparentized with chloroform, and then embedded for a long time, dyed, mounted and made into tissue sections.

实验组经过常规固定，而后应用改良脱钙液（甲酸10 mL、浓盐酸(相对密度1.19)8 mL、氯化铝3 g、体积分数为0.4的甲醛20 mL、冰乙酸3 mL，加生理盐水至总体积200 mL）脱钙、脱水、氯仿透明、包埋时间延长、染色、封固和切片；对照组进行常规固定、脱钙、脱水、透明、包埋、染色、封固和切片。

Results When the culture medium was supplemented with 0 .05μg/ml sodium selenite,the proliferation of transformed cells was significant ly promoted and the transformation did not change remarkably,when supplemented w ith 0.25μg/ml,the proliferation was promoted but the transformation was inh ibited,and when with 0.75μg/ml and 1.50μg/ml,the proliferation was not pro moted and the transformation was strongly inhibited.Big dose of (1.50μg/ml) sodium selenite m ade the nuclei of the untransformed lymphocytes gather together like a few grape s doing,and even caused them to fuse.

结果 在培养物中添加0.05μg/ml亚硒酸钠能显著促进转化细胞增殖；添加0.25μg/ml可促进转化细胞增殖，但显著抑制细胞转化；添加0.75μg/ml和1.50μg/ml，不仅不能促进细胞增殖，而且强烈抑制细胞转化。1.50μg/ml剂量的亚硒酸钠使非转化淋巴细胞发生葡萄状聚集甚至核融合。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

The results were as follows:The first, there were significantly differences percent between 0.6μg/mlgroups and other three groups (0.2μg/ml groups, 0.4μg/ml groups and 0.8μg/mlgroups) in 2 hour groups; and there were significantly differences between0.4μg/ml groups and other three groups in 4 hour groups; and there weresignificantly differences between 0.6μg/ml groups and other three groups in 6hour groups; and there were significantly differences between 0.4μg/ml groupsand other three groups in 8 hour groups on the metaphases (P＜0.05), in theexperiment of the effect on the mouse 4- cell embryo stage single blastomere ofcolchicine with different concentrations and duration, by x~2 statistical analysis.

实验结果如下：1、在确定秋水仙素不同处理浓度和时间对小鼠4-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，阻断培养2小时组中0.6μg／ml浓度组与其他三组差异显著(0.2μg／ml组、0.4μg／ml组和0.8μg／ml组)；阻断培养4小时组中0.4μg／ml浓度组与其他三组差异显著；阻断培养6小时组中0.6μg／ml浓度组与其他三组差异显著；阻断培养8小时组中0.4μg／ml浓度组与其他三组差异显著(P＜0.05)。

By chicken embryo, minimum concentration of medicine of directly inactivated test、 treatment test and preventive test is 7.5mg/ml、 62.5mg/mland 31.25mg/ml;By CEF, minimum concentration of resiting NDVF_(48)E_8 and moderate virulence strain is 10mg/ml、 50mg/ml and 25mg/ml. However,the result of complex prescriptionpreventing and curing NDVF48Ej$ is not ideal.

在鸡胚上抗病毒试验中，对三株病毒起直接灭活、治疗和预防作用的最低药物浓度分别为7.5mg/ml、62.5mg/ml和31.25mg/ml；在鸡胚成纤维细胞上抗病毒试验中，对强强毒株和中等毒力株起直接灭活、治疗和预防作用不引起细胞病变的最低药物浓度分别为10mg/ml、50mg/ml和25mg/ml。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。