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Oxford cup in vitro antibacterial medicine cup showed that it had vitro inhibitory action on beta streptococcus and colibacillus with crude drug content of 0.5g/ml; it had vitro inhibitory action on staphylococcus aureus,pneumococcus, beta streptococcus and colibacillus with crude drug content of 1.0g/ml,and decreased the death rate of mice caused by staphylococcus aureus.

牛津杯法体外抗菌试验表明0.5g生药量/ml时对乙型链球菌、大肠杆菌有体外抑制作用，1.0g生药量/ml时对金黄色葡萄球菌，肺炎球菌，乙型链球菌，大肠杆菌有体外抑制作用，降低金黄色葡萄球菌引起的小鼠病死率

Methods A total of 56 patients with pollinosis were divided into two groups at random, i.e. treatment group n=32 and control group n=24. The treatment group was treated with compound glycyrrhizin injection 40 ml, added to 250 ml of 5% glucose solution by intravenous drip once per day for one week, in addition to cetirizine, vitamine C and calcium ion.

56例花粉症患者随机分为两组，治疗组32例，对照组24例，治疗组除应用盐酸西体利嗪片，维生素C片，钙剂等治疗外，应用美能注射液40 ml 用5%葡萄糖注射液250 ml 稀释后，静脉滴注，1次/d，连用1周；对照组不用美能注射液，其余治疗与治疗组相同。

The results showed that the Saccharopolyspora spinosa SP06081 protoplast yield was the highest under these conditions: the collected mycelium from SP06081 grown in Tryptic Soy Broth medium with 0.2% glycin for 48 h was treated by 0.1 mg/mL lysozyme at 28oC for 20 min, then plated on the R2YE medium with sucrose as osmotic stabilizer, the number of regeneration protoplast was up to 108/mL. The protoplast-regenerated strains exhibited changes in morphology and antibiotic production, 29.3% protoplast-regenerated strains was characterized by loose mycelium and abundant broken branches as did their parent. Among them, 58.2% strains presented the trend to positive variation in spinosad yield, with the highest spinosad yield of up to 582.0 mg/L, 85.6% higher than that of their parent.

结果表明：菌体在添加0.2%的甘氨酸的TSB培养基中培养48 h收集， 0.1 mg/mL 溶菌酶， 28oC作用20 min制备原生质体，将原生质体涂布于以蔗糖为渗透压稳定剂的R2YE培养基中，原生质体再生数目最多，达108个/mL以上；原生质体再生菌株在形态和抗生素产量上产生分化， 29.3%的再生菌株形态上保持与亲本菌株一致，具有菌丝松散，断裂分枝多的特点，其中53.2%的再生菌株多杀菌素产量变异向正方向移动，最高产量达到582.0 mg/L，比亲本菌株提高85.6%。

The dissolubility of PPLA in water was (2.16～4.32)×10－2 mg·mL－1 according to eye judgment. The critical micelle concentration was 2.16×10－2 mg·mL－1 obtained from a fluorospectrophotometer. The self-assembled aggregates of PPLA in water were nanoscale spheres observed by a transmission electron microscope. The particle size and zeta potential tests by dynamic light scattering revealed that during preparation, the ratio of hydrophilicity/hydrophobicity, the aqueous medium and the pH had great influences; while dilution, freezing and pH change to basic condition had no effect after preparation, but pH change to acidic condition made the nanoparticles precipitate.

目测法得到其溶解度为(2.16～4.32)×10－2 mg·mL－1；荧光法得到聚合物的临界胶束浓度为1.12×10－3 mg·mL－1；透射电子显微镜观察显示该聚合物在水中的自组装聚集体为纳米级球形；动态激光光散射测试微球的粒径和Zeta电位发现，在微球的制备过程中，聚合物的亲/疏水性比例、水相介质及水溶液的pH值对它影响显著；而制备后，稀释和冷冻对它无显著影响，改变微球的环境pH值至酸性，出现聚集，至碱性无影响。

Addition of 3μg/ml CB to CM containing 0.625μg/ml CHX increased the proportion of oocytes with two pronuclei (38. 9% vs 15. 1%, P＜0.05) and developed into morulae/blastocysts (40.7% vs 26. 8%, P＜0.05), but did not affect the percentage of oocytes cleaved and total pronucleus rate.

如果在含有0.625μg/ml CHX中添加3μg/ml CB，可明显提高双原核的形成率（38.9％vs 15.1％，P＜0.05）和桑椹胚/囊胚发育率（40.7％vs 26.8％，P＜0.05），但对其激活分裂率和总原核形成率无明显影响（P＞0.05）。

The anodic peak current was found to be proportional to the concentration of pyridoxine hydrochloride from 0.01 to 1.03 mg/ml, with a detection limit of 1.34 g/ml.

研究表明，阳极峰电流与维生素B_6的浓度在0.010-1.03 mg/ml范围内存在线性关系，检测下限为1.34μg/ml。

In the biochemical analysis aspect, it is first time to use the simple fluorescence spectrophotometer to determine the protein in human serum by red alizarin as the probe of the RLS. The experiment shows that the system HSA-red alizarin has the maximum intensity of the RLS in Britton-Robison buffer of pH4.10 at maximum wavelength of about 360nm. The method has better linear range within 0.040-10.0ug/mL, determination limit is 0.040ug/mL. Moreover, We have selected three other dyes of chromotrope 2R, acid chrome dark blue and pyrocatechin violet as the probes of the RLS in determining protein in human serum and achieving satisfactory results.

在生化分析方面，作者首次使用简易960型荧光分光光度计，利用茜素红作为共振光散射光谱探针对人血清中的蛋白质含量进行了测定，测定结果显示在pH4.10的Britton-Robinson缓冲溶液，波长360nm处出现最大的共振光散射强度，其线性范围为0.040～10.0μg／mL，检出限为0.040μg／mL；另外，作者还筛选了其他三种可用作蛋白质测定的共振光散射光谱探针的有机染料：变色酸2R、酸性铬深蓝、邻苯二酚紫，并深入研究了测定的适宜条件和干扰情况，将这三种有机染料用于人血清中蛋白质含量的测定，均取得了满意结果。

Results the optimized culture conditions as follows: 10 g/l glucose, 10 g/l yeast extract powder, alcohol=10 %, ph 5.5 , culture 5 d quiescently with 50 ml medium in 500 ml flask.

结果优化培养条件为：葡萄糖10 g/l，酵母膏10 g/l，乙醇10%，ph 5.5，装量50 ml/500 ml，静置培养5 d。

The effects of operation parameters on separation of fatty acid methyl ester from rapeseed oil deodorizer distillate by molecular distillation were investigated for further utilization of RODD. The results indicated that the recovery of FAME was 41 g/(100 mL) when pressure was 5.2 Pa, evaporating temperature was 120℃, feed temperature was 70℃, wiper rolling speed was 150 r/min, feed rate was 120 mL/h. The biodiesel was analyzed by GC-TOFMS.

为了实现脱臭馏出物的清洁加工，运用分子蒸馏技术分离菜籽油脱臭馏出物中合成的生物柴油，考察了分子蒸馏的操作参数对生物柴油回收率的影响，研究结果表明：当系统压力为5.3 Pa，蒸发面温度120℃，进料温度70℃，刮膜转速150 r/min，进料速率120 mL/h时，生物柴油回收率达到41 g/100 mL。

Combined with the vacuum harvesting device, it could automatically concentrate 350-liter of the rotifer culture with a density of about 250 rotifers/ml to about 15,000 rotifers/ml in less than 13 minutes.

结合真空收获装置与滚筒过滤机而成的自动化系统可以在13分钟内将350公升的轮虫由密度大约250/ml浓缩到大约15，000/ml。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。