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The radical scavenging activity of Laminaria japonica pigments was studied by the DPPH2,2-Diphenyl~(-1-picrylhydrazylassay. The total pigments showed a strong DPPH radical scavenging activity of 65.819% with the concentration of 0.52mg.ml~(-1). The DPPH radical scavenging activity of fucoxanthin was 19.85% with the concentration of 0.27mg.ml~(-1). The antimicrobial activity of pigment in Laminaria japonica was studied by the method of round paper. The results showed that the pigment extracts have activity against Escherichia coli, Pseudomonas aeruginosa, Saricina flava and Saccharomyes cerevisia. The antibacterial activity of total pigments for Saccharomyes cerevisia was the strongest with an inhibition diameter of 18.9 mm; and the antibacterial activity of fucoxanthin for Saricina flava was also much significant with an inhibition diameter of 10.2 mm.

测定了它们对自由基的清除率和对四种细菌的抑菌效果，研究结果表明，总色素和褐藻黄素的浓度与自由基清除率成正比，当总色素浓度为0.52mg.ml~(-1)时，清除率达到65.819%，高于陆生植物黑米色素和紫甘薯色素；当褐藻黄素浓度为0.27mg.ml~(-1)时，自由基清除率达到19.85%，低于紫苏色素、紫甘蓝色素、黑米色素、紫甘薯色素；对大肠杆菌、绿脓杆菌、八叠球菌、金黄葡萄球菌的抑菌实验结果显示，海带总色素对金黄葡萄球菌的抑菌活性最强，抑菌圈直径为18.9mm；褐藻黄素对八叠球菌的抑菌活性相对较强，抑菌圈直径为10.2mm。

Results: The autofluorescence of M. furfur and M. japonica standard trainsrespectively in 4th and 6th day are stronger than that in other days, andautofluorescence of colonies become weaker in an hour. Theautofluorescence of Malassezia colonies put in 3% glutaraldehyde, pH4.0HCL, 1280μg/mL and 64μg/mL fluconazol solution, 0.3% methylene blueseparately get stronger, and that in 10% KOH get stronger at beginning thenbecome weaker 2 days later. C. albicans, S. schenchii, T. rubrum, T.tonsurans, A. terreus, A. fumigatus cultured on SDA all haveautofluorescence, and which are weaker than that of Malassezia.

结果：糠秕马拉色菌和日本马拉色菌2株标准株的自发荧光强度分别在培养第4和8天时最强，菌落的自发荧光在1小时内逐渐减弱；3％戊二醛溶液、pH4.0盐酸溶液、1280μg／mL和64μg／mL氟康唑溶液、3％美蓝溶液处理后马拉色菌自发荧光都增强，10％氢氧化钾溶液中的马拉色菌在开始时荧光增强，2天后减弱；白念珠菌、申克孢子丝菌、红色毛癣菌、断发毛癣菌、土曲霉、烟曲霉等在培养条件下有自发荧光，但都较马拉色菌弱。

The geometric mean titers of ELISA antibody and NA were 3.8 EU/ml and 5.7 IU/ml, respectively.

ELISA检测的抗体和中和试验检测的抗体平均滴度分别为3.8EU/ml和5.7IU/ml。

Results: The linear range of gramicidin was 0.02~0.06U/mL, The linear range of tyrothricin was 0.1~0.3 U/ml, The average recovery rate of the standard curve assay was 101.7%(n=15, RSD=3.4%).

结果：短杆菌肽线性浓度为0.02~0.06U/mL，短杆菌素线性浓度为0.1~0.3U/mL，标准曲线法的平均回收率为101.7%(n=15， RSD=3.4%)。

The detection limit of the method was 0.3 ng/mL using 4 mL hemolymph samples and injections of 2 μL of the final 25 μL extract.

若取血淋巴样品4 mL，从最终25 μL提取物中注入2 μL，此方法的检出下限为0.3 ng/mL。

The best results were obtained with 40 mg cellulose/2.5 mL water at 380 ℃ and 16 s, affording oligosaccharide recovery above 29%, or 80 mg cellulose/2.5 mL water at 380 ℃ and 18 s, affording hexose recovery above 39%.

最优水解条件研究显示，在380 ℃， 40 mg纤维素/2.5 mL水条件下反应16 s可获得最大的低聚糖产率，为29.3%，在380 ℃， 80 mg纤维素/2.5 mL水条件下反应18 s可获得最大的六碳糖产率，为39.2%。

The mutant was cultured on the rotating shaking bed at the speed of 200 r/min under 25℃for 72 hours,with inoculum size 8% and medium volume 40 mL/500 mL.

菌株于25℃在200 r/min的旋转式摇床上振荡培养72 h，接种量为8%，装液量为40 mL/500 mL三角瓶。

Median serum 25D level was 29 ng/mL (interquartile range, 21 - 37 ng/mL).

血清 25维生素D含量的中位数为29 ng/mL，（四分位距为21 - 37 ng/mL）。

In the EPC repair group, 1010/L passage 3 rabbit bone marrow mononuclear cell suspension was injected to two anastomotic stomas of the new urethra, 0.1 mL for each stoma. The subcutaneous tissue was interruptedly sutured to the formed urethra using 0/6 DG suture. In addition, 0.5 mL bone marrow mononuclear cell suspension was added to the region between each anastomotic stroma and newly repaired urethra. The same procedure was performed in the model group except that bone marrow mononuclear cell suspension was replaced by cell-free medium.

细胞修复组将传至第3代的兔骨髓源单个核细胞浓度调整至1010 L-1，注射于新尿道两端的吻合口处，每处0.1 mL，0/6DG线间断缝合皮下组织覆盖成形后的尿道，并在近吻合口处和新修复尿道之中间处注射细胞悬液，每处0.5 mL；模型组在同样位点注射等量的空白细胞培养液。

Experimental results indicated that the silver nanoparticles of 20 μg/mL could inhibit completely the growth of 10^6 cfu/mL cells in liquid LB medium. The growth curves showed that silver nanoparticles prolonged the lag phase of E. coli, and the higher of the concentration of silver nanoparticles, the longer of the lag phase of E.

纳米银对大肠杆菌的抑制生长曲线的结果表明，20μg/mL的纳米银能够完全抑制10^6 cfu/mL的大肠杆菌细胞生长，纳米银使大肠杆菌的延滞期加长，并且纳米银浓度越高，延滞期越长。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。