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Transfer 2.0 mL of this solution to a 100-mL volumetric flask, add 5.0 mL of Internal standard solution , dilute with pH 7.0 Buffer to volume, and mix.

供试品溶液1的制备（针对注射用单次量容器中的样品

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

The results showed that by using ethanol-alkali method to prepare cold-water-soluble starch, the impact of ethanol concentration and reaction time on the solubility of wheat granular cold-water-soluble starch was significant; the appropriate preparation conditions for wheat cold-water-soluble starch was on the reaction temperature of 65℃, ethanol concentration of 80 mL/l00 ml, reaction time of 80 min, alkali concentration of 10 g/100 ml, under this condition the solubility of the product was up to 81.44%.

结果表明：采用乙醇－碱法制备小麦颗粒状冷水可溶淀粉，乙醇体积分数和反应时间对产物溶解度的影响显著；小麦颗粒状冷水可溶淀粉的适宜制备条件为反应温度65℃，乙醇体积分数80%，反应时间为80min，碱浓度10g/100 ml，其溶解度可达81.44%。

An impregnating solution for hydrogenating or hydrocracking catalyst contains the oxide of metal in group VIB (0.08-0.35g/ml), the oxide of metal in group VII (0.01-0.5g/ml) and citric acid (0.05-0.3g/ml).

本发明涉及一种加氢催化剂，特别是加氢处理催化剂所用浸渍溶液及其制备方法。此溶液中含ⅥB族金属氧化物为0.08～0.35g/ml，Ⅷ族金属氧化物为0.01～0.10g/ml，柠檬酸为0.05～0.30g/ml。

Methods: into a series of 10 ml colorimetric tube were added different aliquots of the 1,8 -dihydroxyanthraquinone standard solution,2.0 ml of naac-hac buffer solution(ph=3.60),2.0 ml of ethylacetate.

于10.0 ml比色管中加入一定量的1，8-二羟基蒽醌标准溶液，ph值为3.60的naac-hac缓冲溶液2.0 ml，有机溶剂乙腈2.0 ml，最后定容至10.0 ml；同时做空白对照，在最大激发波长为440 nm，最大发射波长为521 nm处测定一系列溶液的荧光强度。

RDSSF could increase the incorporation of 〓H-Thymidine into DNA of Africa green monkey kidney cells (Vero E6) within the range of 1ug/ml～6ug/ml, but beyond the concentration of 2ug/ml, there is no increase of biological activity. RDSSF is stable within a range of pH 5.0～9.0 and sensitive to proteinase K and temperature.

RDSSF在体外可显著提高非洲绿猴肾细胞DNA中〓H-TdR掺入量，RDSSF浓度在1ug/ml～6ug/ml时活性明显，浓度达到2ug/ml后，增加RDSSF含量其活性不再增加；该物质在pH5～9范围内生物活性稳定；对蛋白酶k敏感；不耐热。

Versions. In addition, the XD-11 fragrance pump is available in three dosages: 70 ml (2.5 fl. oz.), 100 ml (3.5 fl. oz.) and 120 ml (4.25 fl. oz.).

版本。此外，微型- 11香水泵三种剂量：70毫升（2.5液体盎司），100毫升（3.5液体盎司）和120毫升。

Cell cultures were subcultured every week by transferring 2 ml of 1-week-old culture to 100 ml of fresh medium in a sterile 250 ml flask.F. oxysporum f.s.

细胞体外每周转2毫升1周龄文化100毫升的新鲜培养基育250毫升烧瓶。

Coconut Shower Cream 250 ml, Coconut Soap 100 g, Coconut Body Scrub 50 ml, Coconut Body Butter 50 ml and Cream Two-Tone Bath Lily.

椰雨霜250毫升，椰子肥皂100克，椰子身体去角质50毫升；椰子身体黄油50毫升，奶油双音和百合浴。

Results showed that the laccase activity could be significantly improved by lignin residue. When 6 g/L lignin residue was added to culture medium in an 250 mL Erlenmeyer flask, the laccase activity reached 7005.6 IU/ml which was 5.0 times as high as that of the control whose activity was 1390.1 IU/ml.

在摇瓶条件下，当木质素沉淀物用量为6g／L时，所产漆酶活力为7005.6IU／ml，而不加木质素沉淀物时的产酶活力仅为1390.1IU／ml，酶活提高了5.0倍。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作： 1 回顾了直线电机发展历史，电机的驱动技术演变，特别是针对直线电机的驱动，简要介绍了直线感应电机驱动电梯的优点和不同结构类型，对传统的v／f控制，基于空间矢量法的v／f控制，矢量控制，和基于电压空间矢量的直接转矩控制进行了比较，确立了本课题的研究主题：直线感应电机双边直推式驱动电梯的全数字变频控制。

The article combines with the treatment of a superficial civil air defense work to introduce how the grouting method to improve the performance of the backfill soil.

文章从治理漂浮人防工事的角度提出了注浆技术在改善回填土性质方面的应用，并详细阐述了注浆技术的施工流程。