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Then, they were transferred into the mycelium culture fluid which includes 30% sucrose and 1% or 1.5% glycin under the condition of 20℃, 150 rpm for 48 h. In order to obtain protoplasts of mass and high quality, the strain F46 protoplasts given after combination use of the 10 mg·mL-1 lysozyme and 10 mg·mL-1 snailase at 34℃for 3 h; and the SC1 was treated by 10 mg·mL-1 lysozyme at 34℃for 2.5 h.

确定了双亲原生质体形成与再生的最佳条件：菌株F46、SC1采用二次菌丝培养，即在蔗糖含量20%菌丝培养液里，25℃条件下150 rpm培养48 h；然后按15%的接种量转接到蔗糖含量30%，甘氨酸含量分别为1%、1.5%的菌丝培养液中，同样条件下，继续培养48 h；在34℃条件下，菌株F46用等量混合的蜗牛酶和溶菌酶20 mg·mL-1，菌株SC1用10 mg·mL-1溶菌酶，分别酶解3 h、2.5 h，不仅有大量的原生质体形成，且再生率也高。

Zak mixed 840 ml of vermiculite with 60 ml of finely ground peat moss in the flask, moistened this substrate with 550 ml of MMN nutrient solution, capped the flask with an inverted glass having a cheesecloth-wrapped cotton plug cemented to the bottom, and autoclaved the assembly for 45-60 min at 120℃.

萨克氏混合840 ml的蛭石与60 ml磨细的泥碳土於烧瓶中作为基质，以550 ml的MMN营养液润湿之，再以纱布包棉花塞黏於玻璃杯倒盖之，并将整组器具於120℃高温高压灭菌45-60分钟。

Synthesis apparatus Zak mixed 840 ml of vermiculite with 60 ml of finely ground peat moss in the flask, moistened this substrate with 550 ml of MMN nutrient solution, capped the flask with an inverted glass having a cheesecloth-wrapped cotton plug cemented to the bottom, and autoclaved the assembly for 45-60 min at 120℃.

萨克氏混合840 ml的蛭石与60 ml磨细的泥碳土於烧瓶中作为基质，以550 ml的MMN营养液润湿之，再以纱布包棉花塞黏於玻璃杯倒盖之，并将整组器具於120℃高温高压灭菌45-60分钟。

Pretiosum were continuously provided with tetracycline/30% honey water (1mg/ml、10mg/ml and 100mg/ml) for 10 generations only a few males and intersexes appeared in each generation, most of the progeny were still females.

而被1mg/ml、10mg/ml 和100mg/ml的四环素（四环素量：30℅的蜂蜜水）连续处理10代后，每代均出现了少量的雄蜂和雌雄嵌合体。

Difference of cell-free DNA concentrations was analyzed by one-way analysis of variance using SPSS 10.0 software. There is a significant difference if p value was less than 0.05. The results showed that: 1 DNA concentrations in cell-free lavages supernatants in central lung cancer, peripheral lung cancer and pulmonary benign lesions are (0.83±0.34)μg/ml,(0.66±0.31)μg/ml and (0.59±0.37)μg/ml, respectively.

研究结果如下： 1中心型肺癌支气管灌洗液上清游离DNA量为(0.83±0.34)μg/ml，周围型肺癌为(0.66±0.31)μg/ml，肺部良性病变为(0.59±0.37)μg/ml，经单因素方差分析发现三者支气管灌洗液游离DNA量没有显著差异。

Sowe selected the colchicine optimum parameters were 0.6μg/ml and 6 hour culturedduration.The second, there were no significantly differences between the four groupsin 2 hour groups; and there were significantly differences between 0.2μg/ml groups and other three groups in 4 hour groups; and there were no significantlydifferences between the four groups in 6 hour groups; and there were significantlydifferences between 0.4μg/ml groups and other three groups in 8 hour groups onthe metaphases (P＜0.05), in the experiment of the effect on the mouse 8-cellembryo stage single blastomere of colchicine with different concentrations andduration by x~2 statistical analysis.

根据实验的实际情况，适宜制备小鼠4-细胞期胚胎单卵裂球染色体标本的秋水仙素的处理浓度和时间是0.6μg／ml和6小时。2、在确定秋水仙素不同处理浓度和时间对小鼠8-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，阻断培养2小时组中四个浓度组差异均不显著；阻断培养4小时组中0.2μg／ml浓度组与其他三组差异显著；阻断培养6小时组中四个浓度组差异均不显著；阻断培养8小时组中0.4μg／ml浓度组与其他三组差异显著(P＜0.05)。

Methods Is it fetch urine 50 ml mix even to fetch 10 ml pour scale into in the test tube fresh morning to leave.1 500 r/min.5 min abandons and leavesclearly 0.2 ml.

留取晨尿50 ml混匀，取10 ml倒入刻度管内。1 500 r/min，5 min弃上清留0.2 ml。

Carry out the test for total organic carbon (2.2.44) with a limit of 0.5 mg/l or alternatively the following test for oxidisable substances: to 100 ml add 10 ml of dilute sulphuric acid R and 0.1 ml of 0.02 M potassium permanganate and boil for 5 min; the solution remains faintly pink.

我怎么觉得这个英文的问题很大啊，是缺少内容了吗？

The result showed that LPS inhibited the proliferation of pulp cells when its concentration was 100μg /ml; it could induce the apotosis of the cell when in 250μg /ml and it could lead the pulp cell to die when in 450 μg/ml.

g／ml浓度的LPS可抑制细胞生长，使细胞体积缩小；200μg／ml浓度的LPS可使个别细胞收缩变圆，发生类似凋亡样改变；而400μg／ml浓度的LPS可使细胞肿大、破碎发生坏死样改变。

The improved method selects the absorbing wavelength of 618 nm and the reaction time of 40 min, uses the NH4Cl-NH3 buffer solution (pH=8.0) and BTB alcohol solution (0.4 g/L) respectively of 5 ml and 1.5 ml in the 50 ml volumetric flask, and can test the concentration of imidazolinyl-quaternary-ammonium-salts in range of 0 mg/L to 80 mg/L.

利用改进的显色反应法检测时，吸收波长选择618 nm，显色时间40 min， 5 ml容量瓶中NH4Cl-NH：缓冲液(pH=8.0)和BTB乙醇溶液(0.4 g/L)的添加量分别为 5 ml、15 ml，适用检测的咪唑啉季铵盐浓度为0~80 mg/L。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作： 1 回顾了直线电机发展历史，电机的驱动技术演变，特别是针对直线电机的驱动，简要介绍了直线感应电机驱动电梯的优点和不同结构类型，对传统的v／f控制，基于空间矢量法的v／f控制，矢量控制，和基于电压空间矢量的直接转矩控制进行了比较，确立了本课题的研究主题：直线感应电机双边直推式驱动电梯的全数字变频控制。

The article combines with the treatment of a superficial civil air defense work to introduce how the grouting method to improve the performance of the backfill soil.

文章从治理漂浮人防工事的角度提出了注浆技术在改善回填土性质方面的应用，并详细阐述了注浆技术的施工流程。