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Methods Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of in vitro fertilization-embryo transfer program and were cultured with M199 medium plus various concentration of leptin (0, 10, 30, 100, 300 ng/ml),human menopausal gonadotropin (hMG, 0, 0.1, 0.2, 0.5, 1, 2, 5, 10 IU/ml) and testosterone 100 μg/ml. For 2 days the media were collected for estradiol and progesterone measurements.

方法将来自体外受精-胚胎移植的卵巢黄素化颗粒细胞纯化后，在不同浓度瘦素(0、10、30、100、300 ng/ml)和人绝经期促性腺激素（hMG，0、0.1、0.2、0.5、1、2、5、10 IU/ml）单独或联合作用下进行体外培养，收集培养液，采用放射免疫方法测定颗粒细胞产生雌二醇及孕酮的量。

Two 0.5-ml volumes of an isobutanol internal standard (10 mg/ml water; pipette 12.4 ml of isobutanol and dilute to 1 liter with water) are pipetted into two different 2-dram (7.4-ml) shell vials, one market "known."

将两份0.5毫升的异丁醇内标物(浓度为10mg/ml水，其配制法为：用吸量管吸取12.4ml 异丁醇用水稀释至1L )，用吸量管分别吸入两个不同的2-打兰(7.4毫升)的壳型小瓶中，一个标为&已知&，另一个标为未

Two 0.5-ml volumes of an isobutanol internal standard (10 mg/ml water; pipette 12.4 ml of isobutanol and dilute to 1 liter with water) are pipetted into two different 2-dram (7.4-ml) shell vials, one market "known."and the other "unknown."

将两份0.5毫升的异丁醇内标物(浓度为10mg/ml水，其配制法为：用吸量管吸取12.4ml 异丁醇用水稀释至1L )，用吸量管分别吸入两个不同的2-打兰(7.4毫升)的壳型小瓶中，一个标为&已知&，另一个标为&未知&。

But potentiating effect on IFN-induced growth inhibition was fewer noticeable in 1.4 μg/ml of synthetic peptide.

体外抗病毒结果显示，干扰素在亚保护剂量时(20～70 pg/ml)时，合成肽能提高IFN-α作用细胞后的抗病毒活性，并且合成肽WLDPRH在1.4 μg/ml时， IFN-α抗病毒保护率从40%提高到86%，为最高保护率的最低剂量，但合成肽为1.4 μg/ml， IFN浓度为5～55 ng/ml时，对IFN-α诱导的抗增殖作用不明显。

Results: The growth of NB4 cells was inhibited significantly after treated with berbamine at different concentrations for different time points, the IC50 value was 3.860μg/ml at 48 hours. Morphology analysis showed the characteristics of apoptosis, and the DNA agarose electrophoresis showed the typical DNA ladder. The apoptosis rate increased from 2.83% to 58.44% after treated with berbamine at 12μg/ml for 18 hours. The expression of PML-RARα mRNA presented no significant changes, however, Survivin mRNA was decreased dramatically.

结果：经不同浓度小檗胺处理不同时间后，NB4细胞生长出现明显的抑制，并呈现明显的时间剂量依赖性，48h IC50为3.860μg/ml；细胞形态学观察可见细胞凋亡现象，DNA琼脂糖电泳显示典型的凋亡梯形图：流式细胞仪检测发现细胞凋亡率明显增加，经48h作用后，凋亡率从2.83%增至12μg/ml时的58.44%；虽未发现药物作用后PML/RARα基因表达量的改变，但检测到NB4细胞经药物作用后Survivin基因表达量的减少和Caspase3表达的增加，Caspse3阳性率从对照组的2.06%增至12μg/ml时的70.89%。

An alkaline protease Alcalase AF 2.4 L was used to hydrolyze corn gluten meal and study the effects of pH and enzyme concentration on protein conversion. The optimum technological conditions were obtained in this test as follow: hydrolyzing temperature 55℃,addition of 2 ml alkaline protease(90 947 U/ml),pH=9,concentration of substrate 0.1g/ml,hydrolysis time 1 h.

研究了碱性蛋白酶Alcalase AF 2.4L水解玉米蛋白时，pH值和酶浓度对蛋白质转化率的影响，得到了实验范围内的最佳工艺条件：水解温度55℃，碱性蛋白酶加入量2ml(90 947U/ml)，pH值9，底物浓度0.1g/ml，水解时间1h。

Then, they were transferred into the mycelium culture fluid which includes 30% sucrose and 1% or 1.5% glycin under the condition of 20℃, 150 rpm for 48 h. In order to obtain protoplasts of mass and high quality, the strain F46 protoplasts given after combination use of the 10 mg·mL-1 lysozyme and 10 mg·mL-1 snailase at 34℃for 3 h; and the SC1 was treated by 10 mg·mL-1 lysozyme at 34℃for 2.5 h.

确定了双亲原生质体形成与再生的最佳条件：菌株F46、SC1采用二次菌丝培养，即在蔗糖含量20%菌丝培养液里，25℃条件下150 rpm培养48 h；然后按15%的接种量转接到蔗糖含量30%，甘氨酸含量分别为1%、1.5%的菌丝培养液中，同样条件下，继续培养48 h；在34℃条件下，菌株F46用等量混合的蜗牛酶和溶菌酶20 mg·mL-1，菌株SC1用10 mg·mL-1溶菌酶，分别酶解3 h、2.5 h，不仅有大量的原生质体形成，且再生率也高。

Results Reinfused blood volume in 9 patients was 420～985 ml,average 654ml.

结果：9例病人自体血回输量420～985 ml，平均654 ml，其中再次手术的2例病人回输血量分别为960 ml和985 ml。

The bacterium was incubated in 250 mL Erlenmeyer flask containing 50 mL of above liquid medium and kept shaking at 37℃ for three day（150 rpm）.

於含50 mL较适培养基的250 mL三角锥形瓶中，於37℃振荡（150 rpm）培养3天后，可得较佳之蛋白酶活性（0.11 U/mL）。

Results showed that the laccase activity could be significantly improved by lignin residue. When 6 g/L lignin residue was added to culture medium in an 250 mL Erlenmeyer flask, the laccase activity reached 7005.6 IU/ml which was 5.0 times as high as that of the control whose activity was 1390.1 IU/ml.

在摇瓶条件下，当木质素沉淀物用量为6g／L时，所产漆酶活力为7005.6IU／ml，而不加木质素沉淀物时的产酶活力仅为1390.1IU／ml，酶活提高了5.0倍。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。