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The highest enzyme producer alkalophilic Bacillus sp SHY8-1 among the inductive strains was mutagenized several times, by adding enzyme production repression aegents into screen medium, combined with natural seperation and pressure selection, finally a CMCase-hyperproductive mutant SHY8-1-25 (partitally resistant to catabolite) was obtained, with the yield of CMCase raised to 13-16u/ml, maximally reached 18-20u/ml, while its parent strain 0.654u/ml.

对诱导型产酶菌-嗜碱芽孢杆菌SHY8-1进行了多次诱变改良，采用添加产酶阻遏剂的平板作为筛子，结合反复的自然分离、压力选择，最终成功地获得了高产变异株SHY8-1-25(已部分解除分解代谢阻遏)，产酶量由0.654u/ml提高到13-16u/ml，最高达到18-20u/ml，讨论了诱变机理策略，考察了碳、氮源等对产酶的影响，对培养基和摇瓶培养条件进行了优化。

The cells were preincubated at first in liquid phase for 24 hours at a concentration of 5×106 nucleated cells/ml of RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and concanavalin A5μg/ml of culture medium.

先将脾脏有核细胞(5x10~6/ml)在含有ConA(5μg/ml)的液相中培养24小时，然后收集非粘附细胞，将其接种到含0.88％甲基纤维素、10％FCS，ConA 5μg/ml和15％ConA-LCM的半固体系统中培养，细胞的终浓度为5x10~5/ml。

Methods: Introduce cytopathology experimental methods, human hepatic cancer cell line Bel-7402 co-cultured with Triptolide (10 g/mL, 5μg/mL and 0.25μg/mL), Using MTT method to test the effect of Triptolide on Bel-7402 cell proliferation, using TUNEL technique to test the effect of Triptolide on Bell-7402 cell apoptosis, immunohistochemistry staining method to test Bax and Bcl-2 protein expression.

采用细胞药理学实验方法，人肝癌细胞株Bel-7402与雷公藤甲素（10μg/mL、5μg/mL及0.25μg/mL）共培养，MTT法检测其对Bel-7402细胞增殖的影响，TUNEL技术检测其对Bel-7402细胞凋亡的影响，免疫组织化学染色法检测Bax及Bcl-2蛋白表达。

Result At the end of dioestrus, the estradiol level was increased gradually, and the peak of estradiol appeared from 1 day before oestrus to 3 days after oestrus (52.50±29.26) pg/mL, then the estradiol decreased rapidly, its lowest value at 6-9 days of oestrus (7.20±5.50) pg/mL. The level of progesterone was low before oestrus, and rose gradually at the peak of estradiol and reached the highest value at 9-15 days of oestrus (35.52±7.87) ng/mL, then it decreased gradually.

结果 发情间期的末期，雌二醇开始升高，在发情前1 d至发情开始后3 d内出现雌二醇高峰(52.50±29.26) pg/mL，峰值后雌二醇急剧下降，到发情第6~9天下降至最低值(7.20±5.50) pg/mL；孕酮水平在发情前期较低，于雌二醇高峰时开始上升，发情第9~15天达到最高值(35.52±7.87) ng/mL，此后逐渐下降。

RESULTS The average bacteriacolony forming unit per milliliter in dialysis fluid from the dialyzer inlet , outlet and reverse osmosis was 486 CFU/ ml ,490 CFU/ ml and 103 CFU/ ml , respectively. The common isolated bacteria were Bacillus subtilis , diphtheroid bacilliand some Gram2negative bacilli.

结果 透析器入口处透析液的平均菌落数为486 CFU/ ml ，透析器出口处透析液的平均菌落数为490 CFU/ ml ，反渗水的平均菌落数为103 CFU/ ml ，常见细菌为枯草杆菌、类白喉杆菌及某些革兰阴性杆菌。

The different concentrations of DS are 100μg/ml , 50μg/ml and 25μg/ml.

DS浓度分别为：100μg／ml、50μg／ml和25μg／ml。

The mechanism of GCT restrained by ADM: To determine the mechanism of GCT restrained by ADM, the GCT cells were cul- tured with ADM (the dense of ADM after adding were 0.04μg/ml, 0.4μg/ml and 4μg/ml) for 72 hours. The GCT cell were observed by microscope and electron lens, analyzed by flow cell counter and DNA electrophoresis.

阿霉素抑制骨巨细胞瘤的机理：为探讨阿霉素抑制骨巨细胞瘤的机理，我们用阿霉素与骨巨细胞瘤细胞悬液共同培养72小时（阿霉素最终浓度分别为0.04μg/ml，0.4μg/ml及4μg/ml），搜集细胞，用光镜、电镜观察及用流式细胞仪、DNA电泳进行分析。

At 400μg/mL, the micronucleus ratio was 36.0l‰ and karyokinesis index was only 1.90%. The multi-nucleolus ratio was significantly enhanced at the concentration 200~400μg/mL, and the ratio at 400μg/mL was 26.10‰.

当细交链孢菌酮酸浓度为12.5~100μg/mL时，与对照相比，蚕豆根尖细胞多核仁率无显著差异，多核仁率低于3‰；浓度升高到200~400μg/mL时，细胞多核仁率显著升高，400μg/mL时，多核仁率高达26.10‰。

With the increase of amylum quantity, the color of each the standard solution iodine combined with amylum changes dark and the its absorbance enlarges, the linear part in standard curve enlarges, while the amylum quantity are 20 ml and 50 ml respectively, the length of linear part in standard curve is not changed, so the optimum quantity of amylum is 20 ml.

对于碘标准系列溶液，随着显色剂淀粉用量的增加，溶液的颜色加深，吸光度在增大，标准曲线的线性部分加长，加入淀粉为20 ml和50 ml时，直线部分的长度变化不大，因此，加入20 ml最为理想。

Results:LIF improved the development rate of early embryo at the concentration of 0.1,1 and 10ng/ml(P.05).The enhanced effects of LIF were significant for morula and blastocyst stage,not for 1～8 cell stage of embryos.Conclusion:LIF may improve the development of early mouse embryos at the appropriate concentration.

结果：①0.1ng/ml、1ng/ml和10ng/ml浓度的LIF对小鼠早期胚胎的发育和胚泡的形成有促进作用，0.01ng/ml和100ng/ml浓度的LIF对胚胎的发育无明显影响；②LIF对小鼠早期胚胎发育的影响主要发生于桑椹胚和胚泡期，对1～8细胞期胚胎无明显影响。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。