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The content of purines in soup increased sharply during 10 min, increased slowly during 10 min to 60 min. The content of adenine in carp weighed 200 g increased during boiling process, that of guanine was stable, that of total purines and hypoxanthine decreased. The content of total purines and four purines in Huahuang fish decreased during boil processing.

鱼汤中嘌呤含量在10min内迅速升高，10~60min缓慢升高。200g鲤鱼中腺嘌呤含量在水煮加热过程中升高，鸟嘌呤含量无明显变化，总嘌呤和次黄嘌呤组分含量下降，200g黄花鱼的总嘌呤及4种嘌呤组分含量在水煮加热过程中下降。

When admitted, UREMIA group included 9 cases with end stage renal disease and 5 cases with GFR level between 10～20ml/min, CRF group had 23 cases with GFR level between 20～50ml/min and CRI group had only 3 patients with GFR above 50ml/min. Age、sex、proteinuria、 prevalence of blood hypertension, diabetes and coronary heart disease were all matchable within groups, while hemoglobin level and length of kidney were much lower in uremia group.

各组患者的年龄、性别、蛋白尿程度、高血压和并发冠心病、糖尿病的情况均无不同，但尿毒症组患者的贫血较重、肾脏体积较小；处于肾功能失代偿期的CRF组患者也出现轻度贫血，其程度与GFR下降程度显著相关（R=0.476，P＜0.05）；就诊较早的患者肾功能相对较好。

The single source and single sink network and the multiple sources and multiple sinks network are considered,respectively.We first construct an auxiliary network and convert the min-cost flow problem in origial network into the min-cost circulation problem in the auxilary network,and then find the optimal solution of min-cost circulation problem in the auxiliary network by using out-of-kiltem algorithm.

分别就单源单汇和多源多汇情况构造不同的辅助网络，将原网络中的最小费用流问题转化为辅助网络中的最小费用循环流问题，然后用瑕疵算法求最小费用循环流问题的最优解，这样在求出原网络中最小费用流的同时，也获得了总流量的最优取值。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

The results showed that while the raying distance was 10 cm and an ultraviolet lamp with 20 W acted on the strain of Norcardia corailina 3 min and 4 min, the lethal ratio was high than 90%; While the raying time was 5 min, the lethal ratio was 100%.

结果表明：在紫外灯功率为20w，照射距离为10cm的条件下，诱变时间为3min和4min，致死率均大于90％；而诱变时间为5min，致死率为100％。

Compared with the reference meal, the highest level of vinegar significantly lowered the blood glucose response at 30 and 45 min, the insulin response at 15 and 30 min as well as increased the satiety score at 30, 90 and 120 min post-prandially.

与对照相比，高水平的醋明显降低30和45分钟时的血糖反应和15和30分钟时的胰岛素反应；明显升高餐后30、90和120分钟时饱腹感评分。

Were used as explants to discuss the effect of growth regulators on inducement, multiplication , rootage and callus; and analysed the range of effective factor.

实验结果如下： 1 采用0.10％升汞为消毒剂对外植体进行处理时，茎尖的适宜消毒时间在8 min左右，叶柄为12 min，叶片4 min。

In this research, the activities of CWDE were measured by saccharifying according to the theory that red-brown amino compounds were produced when 3, 5-dinitro salicylic acid and aldehyde were heated together. And the testing conditions were made clear. The activity of Cx should be tested with optimal temperature of 50℃, optimal pH of 4.8, incubation time of 30 min, and optimal substrate of 0.6% CMC-Na. Due to the heat-libality, Cx should be sterilized more than 10 min. The testing conditions of the activity ofβ-G were almost the same with Cx except the substrate of 0.5% salicin and the sterilization time of more than 15 min.

主要取得了一下成果：(1)明确了条锈菌细胞壁降解酶活性测定的条件：羧甲基纤维素酶活性的测定条件为50℃，pH4.8，反应时间30min，底物浓度为0.6%羧甲基纤维素钠，以DNS法测定，且失活酶液的灭活时间应大于10min；β-葡萄糖苷酶活性的测定条件为：50℃，pH4.8，反应时间30min，底物浓度为0.5%水杨苷，以DNS法测定，且失活酶液的灭活时间应大于15min；果胶酶活性的测定条件为：50℃，pH4.8，反应时间1h，底物浓度为0.25%果胶，以DNS法测定，且失活酶液的灭活时间应大于10min。

Pe Min Kam Wan, Lam Kam Sang Nasalin and Wo Chun Tong possess antiallergic activities. Senecio scandens and Selaginella may be the effective ingredients of Wo Chun Tong and Pe Min Kam Wan, and Xanthium sibiricum may also be one of the effective ingredients of Pe Min Kam Wan.

速效鼻敏感丸、和春堂鼻敏清片及林金生鼻敏清胶囊具有抗过敏活性，九里明和卷柏可能为速效鼻敏感丸及和春堂鼻敏清片的有效组分，苍耳子亦可能为速效鼻敏感丸的有效组份之一。

The effects of other cooking methods were investigated: steaming for 0–20 min, microwave cooking for 0–3 min and stir-fry cooking for 0–5 min.

也研究了其他的烹饪方法：蒸0－20分钟、微波0－3分钟和快炒0－5分钟。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。