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F. Nees and their metabolites by HPLC, the mobile phase was acetonitrile-water (0.1% methanoic acid) for gradient elution, Column temperature was 25℃, flow rate was 1.0mL/min, the detection wavelength was at 254nm..

采用HPLC对穿心莲及其代谢产物进行研究,以乙腈-0.1%甲酸水进行梯度洗脱,柱温为25℃,流速为1.0mL/min,检测波长为254nm。

Using UPLC system,WATERS ACQUITY UPLC BEH C~(18)(2.1 mm×50 mm,1.7μm) column and the mobile phase of 1% methanoic acid solution-acetonitrile with the flow rate of 0.6 mL·min~(-1),the determination wavele...

使用 UPLC 色谱系统,BEH C~(18)色谱柱(2.1mm×50mm,1.7μm);流动相为1%甲酸溶液—乙腈;流速为0.6mL·min~(-1);检测波长为280nm。

The experimental conditions were as follows: C18 chromatographic column, mobile phase of MeOH-H2O (both include 0.1% methanoic acid), flow rate at 1.0 mL/min, gradient elution, detection wavelength at 290 nm and room temperature.

采用C18色谱柱,甲醇-水(含0.1%的甲酸)梯度洗脱;流速为1.0mL/min,检测波长290nm;柱温为室温。

The optimal condition of the methanolysis transesterification was as follows: 50% enzyme based on oil weight;methanol/oil mole ratio 3∶1;shaking speed 150 r/min;added water content 5%;reacting at 50 ℃ for 12 h.

最佳工艺条件为:在正己烷的反应介质中,酶量50%,醇油摩尔比3∶1,反应温度50℃,转速150r/min,添加质量分数5%的水分,反应12 h后产物脂肪酸甲酯得率可达92.3%。

Results showed that AOT reversed micellar systems provided a suitable micro-environment for biodiesel production. The optimal parameters were: the ratio of water and AOT 11, AOT concentration 50 mmol/L, temperature 40℃, pH 7, molar ratio of methano and oil 3, and rotating speed 180 r/min. The transesterification rate reached 90% when a 3-step methanolysis protocol was used.

研究结果表明: AOT/异辛烷反胶束体系为Candida sp.99-125脂肪酶催化提供了较为合适的微环境,在W0为11,表面活性剂浓度为50 mmol/L,温度为40℃,缓冲液pH值为7的AOT/异辛烷反胶束体系中,醇油摩尔比为3∶1,摇床转速为180 r/min,采用12h3次流加1 mol当量的甲醇,单批最高酯转化率可以达到90%。

The Mannich-type addition of O , O'-dialky phosphite to 2-fluorobenzaldehyde and 4-trifluoro- methylaniline was carried out in one pot by means of adopting three kinds of green synthetic methods, microwave irradiation, ionic liquid and free solvent-catalyst condition. According to the results, the synthetic method was optimized without catalyst and solvent at 100 ℃ and 15 min, and it was economic, convenient, high yields, timesaving and environment friendly.

采用微波辐射﹑离子液体和无溶剂无催化剂三种绿色合成方法,分别进行了以亚磷酸酯、邻氟苯甲醛和对三氟甲基苯胺为原料的类Mannich一锅法合成反应研究,经合成筛选,优选出控制反应温度100 ℃、无溶剂和无催化剂加热反应15 min的合成方法,实验结果表明该方法经济、简便、产率高、反应时间短及对环境友好。

The separation was achieved within 4 min using a BEH Shield RP18 column (100 mm 2.1 mm, 1.7 μm) with 50 mmol/L monoammonium phosphate solution (pH 6.5) and acetonitrile as the mobile phase.

以BEH Shield RP18柱(100 mm 2.1 mm,1.7 μm)为分析柱,以50 mmol/L的磷酸二氢铵(pH 6.5)和乙腈为流动相进行梯度洗脱,3种磷酸腺苷在4 min内实现了较好的分离。

Methods: The HPLC with a C18 column (150mm×4.6mm, 5μm) was used. Methanol-0.05mol/L Potassium phosphate monobasic buffer solution-Acetic acid glacial (40:60:1) was used as mobile phase. The flow rate was 1.0mL/min, and the detection wavelength was at 333nm.

采用高效液相色谱法,用C18色谱柱(150mm×4.6mm, 5μm),以甲醇-0.05mol/L磷酸二氢钾溶液-冰乙酸(40:60:1)为流动相,流速为1.0mL/min,检测波长333nm。

The chromatography conditions were as follows: methanol - 0.1mol/L monobasic potassium phosphate (50∶50) as mobile phase, flow rate being 1.0ml/min and detecting wavelength was 290nm.

方法采用高效液相色谱法,色谱柱为Diamonsiltm C18柱;流动相:甲醇-0.1 mol/L磷酸二氢钾水溶液(50∶50);流速:1.0 ml/min;检测波长:290 nm。

MethodsHigh Performance Liquid Chromatography was performed Diamonsil C18. The chromatography conditions were as follows: methanol - 0.1mol/L monobasic potassium phosphate (50∶50) as mobile phase, flow rate being 1.0ml/min and detecting wavelength was 290nm.

方法采用高效液相色谱法,色谱柱为Diamonsiltm C18柱;流动相:甲醇-0.1 mol/L磷酸二氢钾水溶液(50∶50);流速:1.0 ml/min;检测波长:290 nm。

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相关中文对照歌词
En Katt På Min Kudde
10 Min With God
Hold Min Hånd
15 Min Flame
Hallo Elskan Min
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I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里,举止就文雅一些,因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计,这件高贵的制服是香港本土制造,是我们第一套不配帽子的制服。

Do not 'inflate' your achievements and/or qualifications or skills .

不要 '夸大' 你的业绩或成果,条件或者技能。