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The results showed that 1 transport of Gly-Pro was slightly quicker when Na+ replaced by K+ during the first period of 20 min;2 Na+ concentration gradient stimulated 4.6-fold of L-alanine transport, but had no effect on transport of Gly-Pro. Harmaline reduced transoport of L-alanine significantly but had no effect on Gly-Pro transport.

结果表明：(1)用K+替代Na+，并没有降低Gly-Pro在BBMV中的转运量，在孵育前20 min时间内，Gly-Pro的转运量稍稍提高；(2)Na+可使BBMV对L-丙氨酸的转运量提高到4.6倍，但不影响Gly-Pro的转运；二氢骆驼蓬碱显著地降低L-丙氨酸的转运，但对Gly-Pro的转运无影响。

HPLC was used to determine harmine concentration in the oral emulsion or in tissues of mice. Stationary phase: Shim pack CLC ODS column; mobile phase: acetonitribe methanol phosphate buffer (pH6.8)(30∶30∶40); mobile speed: 1.0 mL/min; detection wavelength: 300 nm; column temperature: 20℃.

采用高效液相色谱法测定口服乳剂中的harmine含量和各脏器组织中的药物浓度；色谱柱Shim pack CLC ODS柱，流动相为ψ［乙腈∶甲醇∶磷酸盐缓冲液（pH6.8）］=30∶30∶40，流速1.0 mL/min，检测波长300 nm，柱温为室温。

Methods: The chromatographic separation was performed on a Hypersil ODS2 column(250 mm×4.6 mm, 5 μm) with mobile phases of potassium dihydrogen phosphate hexyl amine solution-acetonitrile(74∶26) for the determination of its related substances and methanol-0.05 mol/L potassium dihydrogen phosphate solution(1∶1) for the determination of dexbetamethasone sodium phosphate. The flow rate was 0.8 ml/min, the detection wavelength was 254 nm, the column temperature was 25℃ and the sample size was 20 μl.

色谱柱为Hypersil ODS2柱(250 mm×4.6 mm，5 μm)；有关物质测定的流动相为磷酸二氢钾己胺溶液-乙腈(74∶26)，含量测定的流动相为甲醇-0.05 mol/L磷酸二氢钾溶液(1∶1)；流速为0.8 ml/min，检测波长为254 nm，柱温为25℃，进样量为20 μl。

They were both easily to be dissolved in dilute alkali. When the pH gradually increases, the ring-opening speed of the derivative is higher than that of GB. The derivative was obviously affected by solvent and higher temperatures. When GB was dissolved in PEG, the peak of the derivative disappeared at 50 ℃ for 15 h or at 120 ℃ for 4 h. Besides the main peak of GB, there appeared a small peak with a retention time of 1.2-3.0 min after heating 4 h at 120 ℃. The result showed that the derivative was in a high energy state, and GB had a better stabilization.

溶剂和温度的综合作用对衍生物的稳定性影响更加显著，GB在聚乙二醇溶液中分别于50 ℃下保存15 h和120 ℃下保存4 h后其中的衍生物峰全部消失；将该溶液于120 ℃下保存4 h后分析，除有主峰GB外，在保留时间为1.2～3.0 min范围还伴随有小峰出现，这说明衍生物处于高能态，GB相对较为稳定，两者共存，且相互转化；在特定条件下衍生物能全部转化为GB。

The results showed that the optimal compositions of medium were 15 sweet potato starch hydrolyte, 0.40 (NH4)2SO4, 0.03 KH2PO4, 0.044 ZnSO4-7H2O, 0.025 MgSO4-7H2O, and that the optimal culture temperature and rotation rate were 32 癈 and 220 r/min respectively.

摇瓶发酵最佳培养基：甘薯淀粉15，(NH_4)_2SO_4 0.4，KH_2PO_4 0.03，ZnSO_4·7H_2O 0.044，MgSO_4·7H_2O 0.025。最佳培养条件为32℃，摇床转速220 r／min，装液量100mL／500mL三角瓶，发酵16h后一次性添加CaCO_370g/L。

Results showed that,under conditions of pH=2.70,using 0.085 mol/L PBS as buffer solution,flow rate 0.8 mL/min,sample injection volume 10 μL,column temperature 25 ℃ and the UV detection wavelength 214 nm,the six kinds of hydroxy acid can be...

结果表明，在C18Hypersil ODS色谱柱上，以pH=2.70，0.085 mol/L Na2HPO4-H3PO4缓冲液为流动相，流速为0.8 mL/min，进样量10μL，柱温25℃，紫外检测波长为214 nm时，可以较好地分离和测定化妆品中的6种羟基酸。

However, this effect was reversed at 3 and 10 μg.kg-1.min -1 for the excessive hypotension.

但这一效应在3，10 μg.kg-1.min-1时，由于血压过低而逆转。

The invention use an alcaligenes faecalis as a fermentation strain, using sucrose or glucose commonly used in food industry as carbon source for microbe cultivation, using conventional chemical fertilizer in the agricultural production as nitrogen source and inorganic salt, the medium of microbial growth is prepared by auxiliary yeast powder, aerobic fermentation is processed in the conditions of 30 DEG C, 1.0vvm, 500r/min ventilatiing and stirring, adding specially supplementary food fluid in the fermentation course achieves the following aims:(1) controlling the penetrating pressure of the broth in the hypotonicity condition;(2) controlling the pH of the broth between 4.0 and 7.0;(3) providing abundant nutritious matter such as carbon source and nitrogen source for microbial growth, obtaining Weilan gum broth after 72 hours, obtainin Weilan gum product by drying in 95-115 DEG C and atmospheric pressure.

本发明以一株粪产碱杆菌为发酵菌种，使用食品工业中常用的蔗糖或葡萄糖作为微生物培养的碳源，以农业生产中常用的化肥作为氮源和无机盐，辅以酵母粉配制微生物生长的培养基，在30℃以1.0vvm、500r/min通风搅拌条件下好氧发酵，发酵过程中流加特殊配制的补料液以达到以下目的：(1)控制发酵液的渗透压处于低渗条件；(2)控制发酵液的pH处于4.0～7.0之间；(3)为微生物生长提供丰富的碳源和氮源等营养物质，经72小时得到威兰胶发酵液，在95℃～115℃下常压干燥得到威兰胶产品。

The changes of freezing point and super-cooling point of a series of PM vitrification solutions was investigated in the process of slow freezing at a ratio of 5℃/min. This experiment showed that the vitrification solution over 41% had not freezing point, while the vitrification solution under 40% had freezing point and could ice.

对PM系列玻璃化液在5℃/min慢速降温过程中冰点、过冷点的产生和变化进行研究，发现浓度在41%以上的玻璃化液降温时不产生冰点结晶，而40%以下玻璃化液在慢速降温时在冰点出现了结晶。

C. The formula of Human Development Index of Agro-Eco-TourismLand is:1 A-Amin EI-Elmin , TI-Tlmin3 max-min Imax-Im/7 Tlmax-TImin HDI- Human Development Index; A- Health Level of Indigene; E- Education Index;TI-Tourism Income.

HDI计算公式为：Hol==生A一AmlnEI一E IminTI一T Im in 3 Amax一Amin Elmax一Elmin Tlmax一Tlmin A一该旅游地居民健康状况；EI一智力程度指标； T一旅游从业人员的人均收入；下标min，max一最小值、最大值。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐