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The second reaction mixture consisted of 5uL 10×PCR buffer,0.5uL 20mmol/L dNTP,1uL of primer R2(25umol/L),0.5uL Taq(2.5U),1.5uL product of the first PeR reaction, 43μL of double-distilled water,the whole system was 50μL;The reaction was run under the following conditions:DNA pre-denaturation at 94℃for 5 min;DNA denaturation at 94℃for 30 s,primer annealing at 55.6℃for 1min,extension at 72℃for 30s,for 25 cycles, and extension at 72℃for 5min.

循环的条件为：94℃变性5min，从94℃变性30s、58.8℃退火1 min到72℃延伸30s共25个循环，最后72℃延伸5 min。

ML dichlorovs(30 mg/L) is oxidized by ultrasonic in 100 mL beakers. When power is 200 W,600 W and 800 W,the removal is low,for the largest removal is less than 20% at the treatment of 60 min, while the removal is 48% when power is 1000 W. On the mean while, cost raises a lot. Consequently, treatment of dichlorovs by ultrasonic is worthless.

超声波法在频率20 kHz，声强为0.3 W/cm2，0.9 W/cm2和1.4 W/cm2（对应的超声功率分别为200 W，600 W和800 W）的条件下处理60 min时的敌敌畏的最大去除率都不超过20％；声强为1.7 W/cm2（1000 W）处理60 min时去除率达48％，但此时处理成本较大，不具实际应用价值。

Results All of the tumor was exsected successfully, the average inosculating time was 28 min and average total operation time was 132 min. The postoperative complications was few and no anastomotic stoma fistula, anastomotic stoma narrow, stomal ulcer or chest gastric emptying block was occurred. The average length of stay after operation was 9.7 days.

结果 肿瘤均顺利切除，平均管状胃吻合成形时间28min，平均总手术时间132 min；术后并发症少，无吻合口屡或狭窄、吻合口溃疡、反流性食管炎及胸胃排空障碍发生；术后平均住院时间9.7d。

Determine the best conditions for the emulsification process:"agent in water law," adding emulsifier, emulsifying time for 30 ~ 40 min, stirring rate for 1000 ~ 1200 r / min, emulsion temperature of 85 ~ 95 ℃, stable wax emulsion.

确定的最佳乳化工艺条件为：采用&剂在水中法&加入乳化剂，乳化时间为30～40min，搅拌速度为1000～1200r/min，乳化温度为85～95℃，得到稳定的蜡乳液。

According to the orthogonal experiments, the optimal transesterification conditions for rapeseed oil and methanol catalyzed by sodium methoxide were obtained: molar ratio of methanol to oil 6:1, amount of catalyst CH3ONa 1.2% of the oil weight, molar ratio of the cosolvent isopropylether to oil 1.2:1, reaction temperature 60℃, reaction time 80 min, the low speed of mixing intensity 120 rad/min, The yield of biodiesel was 96.45%.

通过正交试验得出菜籽油与甲醇酯交换反应的最优条件为：醇油比为6：1，甲醇钠催化剂用量为油重的1.2%，共溶剂与油的摩尔比为1.2：1，反应温度60℃，反应时间80min，低速120r/min搅拌强度下，转化率达到96.45%。

Under optimizing the conditions, rare earth elements were sorbed at column completely when test solution was pH 3 and the flow rate is between 6 and 14 mL/min.

优化了纤维柱吸附和洗脱稀土元素的条件，pH 3的稀土待富集试液以6~14 mL/min流速上柱，以流速为1.5~4.0mL/min的10 mL 2.5 mol/L HNO3洗脱后，用ICP-AES测定。

Methods A total of 30 patients receiving open cholecystectomy under the general anesthesia and epidural anesthesia in our hospital were selected.The right internal jugular vein and femoral vein catheterization was performed after the anesthesia induction and intubation to continuously monitor CVP and the inferior vena cava pressure.Meanwhile, the changes in CVP, MAP, HR, IVCP before abdominal opening,upon pulling gallblad-der as well as 5 min, 10 min and 15 minutes after the opening of deep retractors were observed, recor-ded and compared.

选择在全麻加硬膜外麻醉下开腹胆囊切除手术病人30例，麻醉诱导插管后行右颈内静脉和股静脉穿刺置管，连续监测CVP和下腔静脉压，观察并记录开腹前、牵拉胆囊时、深部拉钩牵开后5、10、15 min及关腹后CVP、MAP、HR、IVCP的变化，并进行显著性检验。

PCR reaction procedure was as follows; pre-denaturizing at 94℃ for 5 mm, denaturizing at 94℃ for 30s, annealing at 51-53℃ for 30 a, extension at 72℃ for 50s, 40 cycles; re-extension at 72℃ for 7 min.

PCR反应程序为94℃预变性5 min；94℃变性30s；51~53℃退火30s，72℃延伸50s，40个循环；72℃再延伸7 min。

Method］mscs(5×106) were cultured combining with gdabm in vitro in centrifuge tube, tgf β was added to collagen and attached to gdabm. culture tube was centrifuged in 600 r/min, 10 times/d, 10min per time. the morphological characters, cell proliferation were detected.

方法］将 mscs与复合转化生长因子-β的胶原共同混合后培养于三维软骨支架材料上，离心管内培养，10次／d，以600 r／min离心10 min.8 h、24 h、72 h、 4 d、7 d进行倒置显微镜形态学观察、扫描电镜观察。1～8 d采用mtt法检测细胞增殖活性，描绘生长曲线。

RESULTS: Both HPC and treatment with ruthenium red (5 μmol/L) during the first 10 min reoxygenation improved recovery of LVDP,±dp/dtmax and decreased LVEDP, which was associated with reduced infarct size and lactate dyhydrogenase release.

用TTC染色法测心肌梗死面积。结果：低氧预处理和低氧后复氧期的前10 min用钌红（5 μmol/L）处理均可促进LVDP、±dp/dtmax和LVEDP的恢复，减小心肌梗死面积，减少LDH释放。复氧期的前10 min用精胺(20 μmol/L)处理可减弱低氧预处理的心脏作用。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。