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The plant expression vectors were constructed and two genes were transferred into A. thaliana and N. tabacum, antikanamycin plants were obtained.4. The trials of adventive bud regeneration directly and callus induction was conducted to select out the optimal explants and formula. The results revealed that double scale can produce 13~16 adventive buds per centimeter in MS medium added 10.0 mg/L 6-BA, 1.0 mg/ L NAA, 40 g/L sugar and 7.5 g/L agar. Scale, leaves, scape and different tissue of flower can be induced to produce callus in appropriate medium, but the filament with anther was the optimized explant.
构建了两个基因的pBI121和pCAMBIA1300植物表达载体,并通过农杆菌将上述基因转入烟草和拟南芥中,得到了卡那抗性的再生植株。4、以中国水仙不同部位外植体进行了不定芽直接分化和愈伤组织诱导分化试验,结果表明,双鳞片外植体在MS0+6-BA 10.0 mg/L+NAA 1.0 mg/L+蔗糖40 g/L+琼脂7.5 g/L培养基上可以大量地直接再生,再生芽数量平均可达13~16个/cm双鳞片,比传统的鳞茎盘之间分化提高30~50%;鳞片、叶片、花葶及花部不同组织在适宜的配方中均可不同程度地诱导出愈伤组织,但以带花药的花丝诱导出的愈伤组织最好。
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They were fed with for four additional levels of β-carotene which was 0, 25 mg/kg, 50 mg/kg, 100 mg/kg (air-dry matter of the dietary) within 6 months.
选取20头荷斯坦种公牛,单因子试验设计,随机分为4组,分别饲喂β-胡萝卜素添加水平0、25、50、100 mg/kg的日粮,试验期为6个月。
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Adjusting pH to 12, air-liquid ratio to the range of 3 000~4 000 and temperature to the range of 35~45℃, after the washing wastewater from deposited ammonium carbonate that the initial concentration of ammonia nitrogen was 1 570 mg/L was treated by ammonia stripping, the residual concentration of ammonia nitrogen was controlled to below 100 mg/L and removal efficiency reached to over 94%.
发现pH、温度和气液比是氨氮去除效率高低的关键因素,废水初始浓度对去除率无影响。调节废水pH=12,气液比在3 000~4 000,温度在35~45℃范围,浓度为1 570 mg/L的碳铵沉淀洗涤废水经氨吹脱处理可使出水残余氨氮浓度控制在100 mg/L以下,去除率达94%以上。
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Sixty Wistar rats were randomly assigned to receive sham operation, blockage of unilateral ureteral without treatment, or intraperitoneal injection of amygdalin at a dose of 3 mg or 5 mg, and then sacrificed on days 7, 14 and 21 postoperatively, Pathological changes in the kidney in each group were observed.
用单侧输尿管梗阻的方法建立大鼠肾脏间质纤维化模型。60只Wistar大鼠随机分为假手术组、单侧输尿管梗阻组、苦杏仁甙3 mg腹腔注射组和苦杏仁甙5 mg腹腔注射组,分别于术后7、14、21 d处死动物,观察各组大鼠肾脏病理损害程度。
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Diels (contains ligustilide > 70%), could markedly inhibit the contractile function of isolated uterine smooth muscle of mice in a concentration-dependent manner (the utlimate bath concentration is 1 mg/100 mL~32 mg/100 mL) whether the test sample was isolated from normal or oxytocin treated mice. It proved that angelica oil had significant anticonvulsive effect in uterine smooth muscle of mice.
当归油 (含藁本内酯 ligustilide>70%),用1%吐温-80助溶,无论对正常未孕离体子宫还是对经缩宫素处理的离体子宫,都可剂量依赖性(浴槽终浓度为 1 mg/100 mL~32 mg/100 mL)地抑制子宫平滑肌的收缩功能,其性质与妇痛宁滴丸相似,但作用稍强,表明其有抗子宫平滑肌痉挛作用。
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Among various fruit-growing positions, compared with the abnormal lateral fruitlets, the normal central and lateral fmitlets had higher fresh weight, dry weight and total Ca, Mg, K content of single fruit, and bigger diameter, more vascular bundles, thicker xylem and bigger phloem vascular bundles area of pedicel, but lower content of Ca, Mg in pedicels.
结果表明,随幼果的发育,单个幼果重量和Ca、Mg、K含量呈增加趋势;与发育不正常的边果相比,发育正常的中心果和边果的鲜重、干重、果柄直径、维管束数目、木质部厚度、韧皮部维管束面积和单果Ca、Mg、K总含量较高,而以干重为单位的果柄Ca、Mg含量较低。
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Then, they were transferred into the mycelium culture fluid which includes 30% sucrose and 1% or 1.5% glycin under the condition of 20℃, 150 rpm for 48 h. In order to obtain protoplasts of mass and high quality, the strain F46 protoplasts given after combination use of the 10 mg·mL-1 lysozyme and 10 mg·mL-1 snailase at 34℃for 3 h; and the SC1 was treated by 10 mg·mL-1 lysozyme at 34℃for 2.5 h.
确定了双亲原生质体形成与再生的最佳条件:菌株F46、SC1采用二次菌丝培养,即在蔗糖含量20%菌丝培养液里,25℃条件下150 rpm培养48 h;然后按15%的接种量转接到蔗糖含量30%,甘氨酸含量分别为1%、1.5%的菌丝培养液中,同样条件下,继续培养48 h;在34℃条件下,菌株F46用等量混合的蜗牛酶和溶菌酶20 mg·mL-1,菌株SC1用10 mg·mL-1溶菌酶,分别酶解3 h、2.5 h,不仅有大量的原生质体形成,且再生率也高。
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SOD activity was at peak in 5 mg/L Zn treatmenat, indicating greater ability to eliminate ROS caused by excessive Zn. Zn contents in roots and thalluses of duckweed in vivo increased significantly with increase of Zn concentration in solution at 0-5 mg/L Zn. When Zn concentration was higher than 5 mg/L, Zn accumulation in duckweed was no more increased significantly. And, the plant was damaged by excessive Zn.
结果表明:在Zn浓度为5 mg/L时,浮萍生物量并没有受到明显的影响,表现出较强的抗性;SOD酶活性也最高,表明清除氧自由基的能力也较强;Zn的积累随处理浓度增加而增加,在5 mg/L Zn处理时,也基本达到饱和,超过该浓度,吸收量则无明显的变化,同时浮萍也明显受到伤害。
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Methods: Twenty-four male SD rats were randomly divided into normal control (NC, n=6), protein C activator (PCA, n=6), lipopolysaccharide (LPS, n=6) and LPS+PCA group (n=6). NC group were given normal saline and PCA group were injected protein C activator (0.4 mg/kg) through vein of the tail. LPS group were administered lipopolysaccharide (10 mg/kg, 4h) by intraperitoneal injection method to make the model of septic shock rats. After the animal model was made, LPS+PCA group were injected protein C activator (0.4 mg/kg) through vein of the tail. 30 minutes after the injection, each rat was tested by Medlab biosignal analyzing system for observing mean arterial pressure and left ventricle function, the activity of LDH and iNOS in myocardium and the cuttings of myocardial tissues.
取SD雄性大鼠24只随机分成对照组、PCA组、LPS组和LPS+PCA组四组,对照组尾静脉注射生理盐水,PCA组尾静脉注射蝮蛇毒蛋白C激活物,LPS组腹腔注射LPS (10 mg/kg, 4h)的方法建立败血症休克的实验动物模型,在建立败血症的实验动物模型的基础上,尾静脉注射PCA (0.4 mg/kg)为LPS+PCA组,每只大鼠均于药后30 min时运用Medlab生物信号采集处理系统记录平均动脉压和左心室功能指标,并测定心肌中乳酸脱氢酶活性、诱导性一氧化氮合酶活性及心肌病理切片。
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ABINGDON'S MG Car Club is thrilled to announce that MG Motor UK has chosen MGLive!
位于英国牛津的迷人小镇阿宾登的MG汽车俱乐部近来宣布:MG英国公司将参加MG Live!
- 推荐网络例句
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For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether
年美国个人破产法的一个改动使得破产登记急速下降,而后引起了信用卡大规模的亏损。
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Eph. 4:23 And that you be renewed in the spirit of your mind
弗四23 而在你们心思的灵里得以更新
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Lao Qiu is the Chairman of China Qiuyang Translation Group and the head master of the Confucius School. He has committed himself to the research and promotion of the classics of China.
老秋先生为中国秋阳翻译集团的董事长和孔子商学院的院长,致力于国学的研究和推广。