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Radiography and double fluorochrome labeling were taken. Dogs were sacrifice at 1, 3, 6 months post operation and image analysis were done on undemineralized slices then subject to statistical analysis by SAS software, Version 6. 21. 3. BMSCs in vitro induced and proliferated through complete blood culturing were seeded into tricalcium phosphate , fdDBM and 2 kinds of coralline hydroxyapatite with 200μm and 500μm porosity (C200 and C500) respectively.

将全血培养法体外诱导、扩增培养的BMSCs分别与三磷酸钙(tricalcium phosphate，TCP)、两种孔径的珊瑚羟磷灰石（200μm，500μm）以及制备的fdDBM复合，体外培养4～7日后，进行扫描电镜、组织学观察，并行裸鼠皮下回植，了解包括空白DBM材料在内的5组样本的异位成骨情况，经IMAGER-PRO PLUS软件进行图像分析，并进一步行统计分析。

In this paper we define the concept of Projective Blaschke manifolds and extend the theory of equiaffine differential geometry to the projective Blaschke manifolds. We prved that if M be a complete projective Blaschke n-sphere and its universal covering manifold is isometric to a complete (n+1) dimensional parabolic, elliptic or hyperbolic affine hypersphere, then M is a quotient space of E^n, S^n or D^n by a isometric subgroup of its corresponding spaces.

在这篇文章中我们定义了射影Blaschke流形的概念，将等仿射微分几何的理论推广到了射影Blaschke流形，并证明：如果n维完备射影Blaschke 超球面 M 的通用覆盖流形分别是完备的抛物型、椭圆型或双曲型仿射球，则M分别是n维欧氏空间、n维超球面或n维单位圆盘关于各自空间的一个等距离散子群的商，从而对完备射影Blaschke 超球面进行了分类。

Results show that when absorptive ratio of water is above 550 times with m: m = 1:4, m: m = 4: 3, mass ratio of initiator is 0. 6%, mass ratio of cross - linker is 0.8‰.

实验结果表明：当引发剂占干物料质量的0.6%，交联剂质量占单体质量的0.8‰，单体与淀粉质量比为4，丙烯酸与丙烯酰胺质量比为1.33，产品的吸水率可超过550。

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

Microcells were produced by centrifugation of micronucleated A9 (neo12) cells in Percoll density gradient containing 20 mg/L Cytochalasin B at 39 000 g. The resulting mixture of microcells, whole cells, karyoplasts and cytoplast fragments was filtered through 8 μm and 5 μm size membrane pores sequentially to obtain pure preparation of microcells.

微核化的细胞在含有20 mg/L细胞松弛B的Percoll密度梯度介质中，经39 000g高速离心后，包含微细胞、完整细胞、细胞核和细胞碎片的混合液，依次通过8 μm和5μm孔径的滤膜过滤后可获得纯化的微细胞溶液。

The deflection of plate is trigonometric series as follows:∞∞ mπx nπy w=∑∑ A m =1 n =1 mn sin a sin b It is obvious, every term of this series is satisfied with the boundary conditions of quadrilateral simply supported.

取板的挠度为如下重三角级数∞∞ mπx nπy w=∑∑ A m =1 n =1 mn sin a sin b 显然，该级数的每一项都满足四边简支的边界条件。

In this paper we present an estimate for bounds of module of eigenvalues of the iteration matrix M^(-1)N for a kind of Baily-Crabtree diagonally dominant matrix M.

该文对一类Baily-Crabtree型对角占优矩阵M，给出了迭代矩阵M^(-1)N的特征值模的上下界估计。

Then, under a certain condition, the estimate for bounds of the module of eigenvalues of M^(-1)N is presented when M is a α- strictly diagonally dominant matrix.

并以此为基础，在一定条件下给出了当M是α-严格对角占优矩阵时的M^(-1)N的特征值模的上下界估计。

Particularly, for some generalized diagonally dominant matrix M, upper bounds of the spectral radius of the iterative matrix M~(-1)Nare presented.

对矩阵M-为几类广义对角占优矩阵的情形，给出了迭代矩阵M~(-1)N谱半径的上界估计式。

In this paper,the estimates of upper bounds for the spectral radius of the iterative matrix M~(-1)N for some generalized diagonally dominant matrix M are presented.

对几类广义对角占优矩阵M，给出了迭代矩阵M~(-1)N谱半径的上界估计式，所得结果较已有结果适用于更加广泛的矩阵类。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。