查询词典 M-B

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

In this paper we present an estimate for bounds of module of eigenvalues of the iteration matrix M^(-1)N for a kind of Baily-Crabtree diagonally dominant matrix M.

该文对一类Baily-Crabtree型对角占优矩阵M，给出了迭代矩阵M^(-1)N的特征值模的上下界估计。

Then, under a certain condition, the estimate for bounds of the module of eigenvalues of M^(-1)N is presented when M is a α- strictly diagonally dominant matrix.

并以此为基础，在一定条件下给出了当M是α-严格对角占优矩阵时的M^(-1)N的特征值模的上下界估计。

Particularly, for some generalized diagonally dominant matrix M, upper bounds of the spectral radius of the iterative matrix M~(-1)Nare presented.

对矩阵M-为几类广义对角占优矩阵的情形，给出了迭代矩阵M~(-1)N谱半径的上界估计式。

In this paper,the estimates of upper bounds for the spectral radius of the iterative matrix M~(-1)N for some generalized diagonally dominant matrix M are presented.

对几类广义对角占优矩阵M，给出了迭代矩阵M~(-1)N谱半径的上界估计式，所得结果较已有结果适用于更加广泛的矩阵类。

For comparison, damage radius for microsurgical scissors was 50 μm, and for diathermy, 400 to 850 μm.

与之相对比的是，微手术剪产生的破环半径是50 μm，透热疗法则是400 to 850 μm。

I'm only just beginning To see the real you And sometimes when we touch The honesty's too much And I have to close my eyes and hide I wanna hold you 'til I die 'Til we both break down and cry I wanna hold you 'til the fear in me subsides Romance and all its strategy Leaves me battling with my pride But, through the insecurity Some tenderness survives I'm just another writer Still trapped within my truths A hesitant prizefighter Still trapped within my youth At times, I'd like to break you And drive you to your knees At times, I'd like to break through And hold you endlessly At times, I understand you And I know how hard you've tried I've watched while love commands you And I've watched love pass you by At times, I think we're drifters Still searching for a friend A brother or a sister But then the passion flares again

I'm 唯一正义起点看见真正您并且有时当我们接触 honesty's 太多并且我必须闭上我的眼睛和掩藏我想要拿着您我死的'til 我们划分和哭泣的'Til 我想要拿着您恐惧在我消退的'til Romance 和所有它的战略留下我作战充满我的自豪感但，通过不可靠一些柔软生存 I'm 其它作家仍然设陷井在我的真相之内一名犹豫的职业拳击手仍然设陷井在我的青年时期之内时常， I'd 喜欢打破您并且驾驶您到您的膝盖时常， I'd 喜欢打破并且不尽地拿着您时常，我明白您并且我知道怎么坚硬you've 尝试了 I've 观看了当爱命令您并且I've 观看了爱通行证您时常，我认为we're 漂流物仍然寻找朋友兄弟或姐妹但另一方面激情再飘动

The rubber composition for covering a carcass cord of the present invention comprises 25 to 80 parts by mass of silica, 1 to 15 parts by mass of a silane coupling agent and 3 to 30 parts by mass of an organic vulcanizing agent represented by the following general formula (1):- n - wherein R is -(CH 2 -CH 2 -O) m -CH 2 -CH 2 -; x is an integer of 2 to 6; n is an integer of 10 to 400; and m is an integer of 2 to 5, based on 100 parts by weight of a diene rubber, and a tire prepared by using the rubber composition.

以100重量份的二烯橡胶为基准，本发明的用于包覆胎体帘线的该橡胶组合物包含25～80质量份的二氧化硅、1～15质量份的硅烷偶联剂和3～30质量份的由如下化学式(1)表示的有机硫化剂：- n -，其中R是-(CH 2 -CH 2 -O) m -CH 2 -CH 2 -；x是2～6的整数；n是10～400的整数；并且m是2～5的整数，以及使用该橡胶组合物制备的轮胎。

A rubber composition for a tire improving friction performance on ice, comprising 0.5 to 20 parts by weight of a nonmetallic fiber having an average fiber diameter of 1 to 100 μm and an average fiber length of 0.1 to 5 mm, and 0.5 to 30 parts by weight of a filler having at least 2 projections and an average length of the projections of at least 10 μm based on 100 parts by weight of a diene rubber, and a pneumatic tire comprising the same.

提供了一种改善冰上摩擦性能的轮胎用橡胶组合物，以及含有该橡胶组合物的充气轮胎。一种轮胎用橡胶组合物，以100重量份的二烯橡胶为基准，还含有0.5～20重量份平均纤维直径为1～100μm并且平均纤维长度为0.1～5mm的非金属纤维，以及0.5～30重量份的具有至少2个突起并且平均突起长度至少为10μm的填料，以及一种含有该橡胶组合物的充气轮胎。

The system parameters are determined by optimizing an output-error cost function. To deal with the non-uniqueness of the fully parameterized state-space system, a projected gradient search algorithm is presented by restricting the update of the parameters to the tangent space to the manifold of observationally equivalent state-space systems. The sufficient condition to employ L-M algorithm for optimizing parameters is also introduced. The proposed hierarchical optimization identificationmethod includes two steps: First, the parameter search direction is determined by the proposed adaptive L-M projected gradient approach; Second, the optimum step size is computed according to a line search method.

通过极小化输出误差目标函数获得了系统参数估计；提出了正交梯度搜索方法用于解决系统参数的非唯一性问题，正交梯度搜索的本质是在输入-输出等价类相切平面的正交垂空间更新系统参数；给出了用 L-M 算法进行参数优化的充分条件；提出的系统参数递阶优化辨识方法包括两步：首先用给出的自适应 L-M 算子正交梯度方法确定参数优化方向；其次由一维搜索方法计算最佳步长。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷