查询词典 LP record

LP1846 in the precombustion chamber is initiated by discharging a high current pulse between a pair of coaxial electrodes,antechamber containes an additional LP booster charge.

实验观测到：在液体发射药的可靠点火过程中，存在着无电弧与电弧两种放电方式。

Objective: To construct the prokaryotic expression vector of human cancer-testis gene NY-ESO-1 by the method of LP Recco PCR Cloning.

目的：利用PCR产物靶向克隆法构建人癌－睾丸抗原NY-ESO-1的原核表达载体。

LP Recco PCR Cloning can efficiently and conveniently construct high quality prokaryotic expression vector of human NY-ESO-1 gene, which provide the expression vector to produce pET-15b-NY-ESO-1 fusion protein.

靶向克隆法可快速、高效地构建人NY-ESO-1基因的原核表达载体，为制备pET-15b-NY-ESO-1融合蛋白奠定了基础。

Methods: Total RNA was extracted from esophageal carcinoma tissue, the primer containing special enzyme was designed, NY-ESO-1 fragment was amplified by RT-PCR, and then was cloned into the expression vector pET-15b by LP Recco PCR Cloning. The recombinants plasmid pET-15b-NY-ESO-1 were identified by restriction endonucleases digest analysis and sequence analysis.

从人新鲜的食管癌组织中提取总RNA，通过RT-PCR技术扩增NY-ES0-1基因3'端的340bp片段，采用靶向克隆法将目的基因插入到原核表达载体pET-15b中，得到重组表达质粒pET-15b-NY-ESO-1，经过筛选，挑选出阳性克隆进行XhoⅠ和BamHⅠ双酶切图谱分析、PCR检测和扩增产物核苷酸序列分析等，鉴定所构建的原核表达载体。

MethodUsing the canis MC4R DNA as template,the specific primers were designed. After PCR amplification,product was cloned into pGEX-4T-1 vector by LP Recco PCR cloning technique,the recombinant pGEX-4T-1-cMC4R was transferred to E.

方法以Beagle犬基因组DNA为模板，经PCR技术扩增目的片段，利用LP Recco PCR克隆技术将目的片段直接重组到原核表达载体pGEX-4T-1上，转化到E.coli DH5α，筛选阳性克隆，BamHI、XhoI酶切鉴定，DNA 测序检测插入序列的正确性。

Methods: Five cases were treated by transnasal endoscopic sphenoethmoidal optic canal decompression in clinic. Results: Three of them had their visual acuity improved from NLP, LP to 0.2～0.4 after the operation. the operative opportunity, merits of operative approaches, the key factors of treatment were discussed.

开展了经鼻内窥镜视神经管减压术，结果：采用经鼻内窥镜鼻内进路视神经管减压术5例，其中3例视力由术前的无光感、光感恢复到术后的0.2～0.4，讨论了手术时机、手术进路的优点和不足、影响疗效的主要因素。

If be in,inform LP not to join what field at first, but cast again in the real operation later, this is unallowed.

如果在最初告知LP不投哪些领域，但在后来的实际操作中又投了，这就是不允许的。

LP bug 36374 Unforwarded

未提出的错误 LP bug 33662

When a decision must be made in time, it will be unsignificative to solve it even if it's very precise. These LP models with large scales which one meets in practice, such as the ones for firms, economics, environment, traffic transport and so on, have serial decisions on discrete time points in them. The decision problems can be described with multiple simplex programming ones with special structures.

对于人们在实践中遇到的各种各样的大规模线性规划模型，如企业模型、经济模型，此外还有能源与环境模型、运输模型等等，它们都包含了在离散时间点上进行的系列决策，这些系列决策问题可以描述为具有特殊结构的多级线性规划问题，各级之间又有着一定的关联和影响，同时又相对独立。

Pale yellow, brown and white translucent calli were produced from zygotic embryos which were stored at 4 to 6℃ for 1 to 3 months and then cultured on medium LP+2mg/L 2, 4-D+1mg/L 6-BA in the dark for one month. Only white trenslucent calli were embryogenic.

合子胚在4～6℃低温条件下保存1～3个月后，接种于LP+2mg/L 2，4-D+1mg/L 6-BA的培养基上，黑暗条件下培养1个月后，产生浅黄色、褐色和白色半透明三种愈伤组织，其中白色半透明愈伤组织是胚性愈伤组织。

Metrorrhagia is also gynaecological problems of common diseases.

崩漏是妇科常见病也是疑难症。

WordFTP gives you the convenience and timesaving you need.

wordftp让您的方便和省时您所需要的。