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METHODS: 15 g/L silk fibroin solution was made by degumming, salvation and dialysis. Chitosan was dissolved in 2% acetic acid solution to prepare 25 g/L chitosan-acetic acid solution. Two solutions were mixed to prepare six silk fibroin/chitosan solutions, and mass ratio was 10: 0, 5: 5, 4: 6, 3: 7, 2: 8, 0: 10. These solutions were separately sucked in a 24-well plate. Following exhausting gas vacuole at 4 ℃, precooling was performed at -20 ℃ for 12 hours, followed by cryodesiccate for 30 hours.

通过脱胶、溶解、透析这3个主要步骤制成质量浓度为15 g/L的丝素溶液，将壳聚糖溶解在2%的醋酸溶液中制成25 g/L的壳聚糖-乙酸溶液，然后将两者混合，配制成丝素/壳聚糖质量比分别为10∶0，5∶5，4∶6，3∶7，2∶8，0∶10的6种溶液，分别吸入24孔板中，4 ℃排出气泡后，-20 ℃预冷冻12 h，再冷冻干燥30 h。

Results showed that the frequencies of shoot regeneration of cotyledons explants from 4 d old in vitro grown seedlings were markedly increased from 34% to 46% via a preculture procedure of 2 days period using the media containing the hormone combinations of 2,4 D 0.5-1.2 g/L and 6 BA 0.2 mg/L.

结果表明，苗龄4d的幼苗子叶于含2，4－D0.5－1.2mg／L和6－BA0.2mg／L的培养基培养2或6d后再转到分化培养基上培养，芽再生率提高34％－46％。

The results showed that: the explants of strawberry leaves were rather sensitive to kanamycin, and the corresponding concentration of 20 mg/L could substantially inhibit the growth of non-transformed tisse and be used to screen the leaves of strawberry after transformation. Timentin at the concentration of 300 mg/L could be used to eliminate Agrobacterium tumefaciens and had minor impact on the growth of the explants. The preculture time of 2-4 days was beneficial to improve transformation frequency. Co-culture for 2-3 days not only had higher gus transient expression but also avoid the over-growth of agrobacterium. The bacterial concentration of OD(subscript 600nm) 0.4 was the best inoculum density and 10 minutes was the best inoculation time.

结果表明：草毒叶片外植体对卡那霉素较为敏感，适宜的筛选浓度为20mg/L，可明显抑制非转化组织的生长；300mg/L的羧吩青霉素可有效抑菌，对外植体生长影响也较小；预培养2-4d有利于转化；共培养2-3d有利于提高转化频率并避免了农杆菌的过度生长；OD(下标 600nm)值为0.4的菌液侵染10min效果最佳。

The results showed that the non-embryonic callus were cultured on the MS medium contained 4.0 mg · L-1 2,4-D and 0.5 mg · L-1 6-BA, embryogenic cells appeared in it. Then the single embryogenic cells formed a proembryo composed of many cells through cell division.

结果表明，非胚性愈伤组织转入到MS + 4.0 mg · L-1 2，4-D + 0.5 mg · L-1 6-BA培养基培养后，愈伤组织中先出现一些胚性细胞；然后单个胚性细胞分裂形成一个由多细胞组成的原胚。

The water-soluble, 650ml/L edible alcohol and 950ml/L edible alcohol-soluble extracts of wild Psidium guajava leaves in Panzhihua area may have different hypoglycemic potential.

攀枝花地区野生番石榴叶的水提取物、650ml/L和950ml/L食用乙醇提取物具有不同程度的降血糖作用。

Results: 21 cases of pancreatitis(25%),25 cases of cholecystitis(29.7%),14 cases of pyoperitonitis(16.6%),5 cases of digestive ulcer(6%),11 cases of acute appendicitis(13.7%),8 cases of bowel obstruction(9%), amylase level of 2-12 h, 24-48 h of pancreatitis group was (465.1±211.4),(509.1±89.9) u/l respectively; that of non-pancreatitis group was (381.9±67.7),(293.4±64.7) u/l respectively.

结果：胰腺炎21例(25%)，2～12 h、12～24 h血淀粉酶分别为(465.1±211.4)、(509.1±89.9)u/l。非胰源性疾病：胆囊炎25例(29.7%)、急性腹膜炎14例(16.6%)、急性阑尾炎11例(13.7%)、肠梗阻8例(9%)、溃疡病5例(6%)，非胰源性疾病2～12 h、12～24 h血淀粉酶分别(381.9±67.7)、(293.4±64.7)u/l。

The floatation rate of Pd (superscript 2+) reaches 100% when the concentrations of NaNO3, KBr and cetyl pyridinium bromide in solution are 0.05 g/mL, 3.0×10^(-2) mol/L, 5.0×10^(-4) mol/L(pH 4.0), respectively. Pd (superscript 2+) could be separated from Ru (superscript 3+), Al (superscript 3+), Cr (superscript 3+), Ni (superscript 2+), Ga (superscript 3+), Fe (superscript 3+) and Zn (superscript 2+) by floatation quantiatively. A method of flotation separation and enrichment of Pd (superscript 2+) was established.

当溶液中硝酸钠、溴化钾、溴化十六烷基吡啶的浓度分别为0.05 g/mL，3.0×10^(-2)mol/L，5.0×10^(-4)mol/L，在pH 4.0时，Pd(上标 2+)的浮选率达到100%，Pd(上标 2+)可与Ru(上标 3+)，Al(上标 3+)，Cr(上标 3+)，Ni(上标 2+)，Ga(上标 3+)，Fe(上标 3+)，Zn(上标 2+)定量分离，据此建立了浮选分离和富集Pd(上标 2+)的新方法。

Answer: BD 1 2. Refer to the exhi bi t. The access l i st has been confi gured on the S0/0 i nterface of router RTB i n the outbound di recti on. Whi ch two packets, i f routed to the i nterface, wi l l be deni ed? Choose two.

根据下图，该access l i st被配置在路由器RTB的S0/0接口上的OUT方向，哪两个数据包会被DENY，如果被路由到该接口上的话？

The Ca2+ salt solution as a new regenerant was presented in this paper. The influences of the calcium salt proportioning, temperature, dosage and flow rate on anion exchange fiber regeneration were studied. High regeneration can be obtained with 0.5 mol/L CaCl2+8 g/L CaO+6.5 °Bx sucrose solution at 70～80 ℃, the dosage of the Ca2+ salt solution was about half of the raw decoloring syrup, the regenerant flow rate should be lower than that of the raw decoloring syrup. It was shown that stable decolorization rate and lower decline in exchange capacity could be achieved with the new regenerant. It performed more effectively than the traditional sodium salt regenerant with less discharge of waste water.

选用二价钙离子蔗糖溶液为再生剂，再生蔗糖脱色后的强碱性阴离子交换纤维，研究了再生液的配比、再生温度、再生液用量、再生液流速等因素对再生效果的影响，结果表明配比为0.5 mol/L CaCl2+8 g/L CaO+6.5 °Bx蔗糖的再生液在70～80℃温度下，再生剂的用量约为脱色糖汁的一半，流速应低于脱色时的流速时，再生效果优于传统的钠盐再生剂的再生效果，重复再生后的纤维脱色率能保持稳定，交换容量下降少，且再生液能回收利用，废水排放量小，是一种环保型再生液。

The Ca(superscript 2+) salt solution as a new regenerant was presented in this paper. The influences of the calcium salt proportioning, temperature, dosage and flow rate on anion exchange fiber regeneration were studied. High regeneration can be obtained with 0.5mol/L CaCl2+8g/L CaO+6.5 °Bx sucrose solution at 70~80℃, the dosage of the Ca(superscript 2+) salt solution was about half of the raw decoloring syrup, the regenerant flow rate should be lower than that of the raw decoloring syrup. It was shown that stable decolorization rate and lower decline in exchange capacity could be achieved with the new regenerant. It performed more effectively than the traditional sodium salt regenerant with less discharge of waste water.

选用二价钙离子蔗糖溶液为再生剂，再生蔗糖脱色后的强碱性阴离子交换纤维，研究了再生液的配比、再生温度、再生液用量、再生液流速等因素对再生效果的影响，结果表明配比为0.5mol/L CaCl2+8g/L CaO+6.5 °Bx蔗糖的再生液在70~80℃温度下，再生剂的用量约为脱色糖汁的一半，流速应低于脱色时的流速时，再生效果优于传统的钠盐再生剂的再生效果，重复再生后的纤维脱色率能保持稳定，交换容量下降少，且再生液能回收利用，废水排放量小，是一种环保型再生液。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了