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Every explant was pricked with a dissector at the center of one subregion before inoculated on MS+BA 1mg/L+NAA 4mg/L+2,4D 05mg/L medium and incubated in darkness for 7 days,then transfered to MS+BA 1mg/L medium.85% of the explants regenerated somatic embryos directly around the prick after 40 days.

每一叶片在1个亚区中心部位用解剖针刺伤1点后接种于MS＋BA1mg／L＋NAA4mg／L＋2，4D05mg／L＋蔗糖20g／L的培养基上，暗培养7d后，转接到MS＋BA1mg／L＋蔗糖20g／L的培养基上继续暗培养，40d后，85％的叶片在刺伤口周围发生直接类型体细胞胚胎。

The result is measured so that the instrument approves inside error CV0.18%; among them WBC of 5 example inside 14 hours all is counted is 109/L; of 20.1 × 109/L, 2.3 × 109/L, 3.2 × 109/L, 22.8 × 109/L, 15.7 × respectively blood, make water is equational leave large intestine dust rare family name, endotoxin test is positive.

结果测得仪器批内误差CV0.18%；其中14小时内5个样本WBC均数分别为20.1×109/L、2.3×109/L、3.2×109/L、22.8×109/L、15.7×109/L；血、尿均分离到大肠埃希氏菌，内毒素试验阳性。

It is fine to cultivate haleness seeding on MS+6-BA 0.1 mg/L+NAA 0.2 mg/L+sucrose30% medium. It is fine to rhizogenesis inducement on MS+BA0.01 mg/L+NAA0.2 mg/L ++sucrose30% medium.

采用MS+6-BA 0.1 mg/L+NAA 0.2 mg/L+蔗糖30 g/L培养基培养健壮苗较好，最佳生根培养基为MS+6-BA 0.01 mg/L+NAA 0.2 mg/L +蔗糖30g/L。

In nitrogen and phosphorus mixed pollution water, in thewater that the concentration of nitrogen is 21mg/L and the concentration of phosphorus is155mg/L, Eichharnia crassipes and Hosta ventricosa can grow normally. In the water that theconcentration of nitrogen is 210mg/L and the concentration of phosphorus is 31mg/L, thephysiological indicators of Hosta ventricosa is good but the physiological indicators of Eichhomiacrassipes is worse than the other treatment and the root of Eichharnia crassipes was rot when itwas planted in the 7th day, and on the 11th day the plants wilt symptoms, it noted that thistreatment affected the growth of Eichharnia crassipes.

在氮磷复合污染的水体中，凤眼莲和紫萼玉簪在氮浓度21mg／L，磷浓度155mg／L水体中都能够正常生长，而在氮浓度210mg／L，磷浓度31mg／L水体中，紫萼玉簪生长正常，而凤眼莲的生理指标比在其它处理中要差，并且凤眼莲在种植的第7天出现根部腐烂，11天时植株枯萎的症状，说明氮浓度210mg／L，磷浓度31mg／L复合污染在一定程度上影响植物的正常生长。

It turned out reproduction rete of chrysanthemum was raised,the regular,non-plant diseases and insect pests could be offered to the market. Shoot tip were used as explants to study the effects of different sterilization methods, illumination intensity and concentrations of 6-BA and NAA on shoot propagation. Rooting with perlite, vermiculite and river sand instead of agar was studied. In the process of preliminary multiplication, the effect of using 0.1% HgCl_2 in sterilization of explants was better than using saturated bleaching solution. The highest multiplication rate was found under whole illumination. MS media with 6-BA from 0.2 mg·L~(-1) to 5 mg·L~(-1) and NAA from 0.05 mg·L~(-1) to 2 mg·L~(-1) were tested and as a result, the highest differentiation rate and the multiplication rate was reached with 0.2 mg·L~(-1) 6-BA, 0.05 mg·L~(-1) NAA.

本实验以菊花的茎尖为外植体，研究了不同灭菌方法，光照强度，全光照、半光照和弱光处理以及不同6-BA和NAA浓度及配比对于试管苗增殖的影响，并研究了珍珠岩、蛭石和河砂等琼脂替代物对于菊花生根的影响，结果表明：在初代培养物建立的过程中采用0.1％升汞进行表面灭菌的效果好于饱和漂白粉的灭菌效果；在全光照条件下外植体的增殖倍数最高；在试管苗增殖培养的过程中，以MS为基本培养基，并在培养基中添加0.2～5mg·L~(-1)6-BA和0.05～2mg·L~(-1)NAA，其中0.2mg·L~(-1)6-BA和0.05mg·L~(-1)NAA最适于外植体的分化和增殖，其分化率为100％，增殖倍数为12。

Results showed that green firm callus and rooting were obtained with treatments of NAA 0.5-3.0mg/L, in which the NAA 0.5mg/L treatment appeared the optimal rooting result of 90%. White loose callus was obtained with treatment of 2,4-D0.1-3.0 mg/L.Callus can not be induced by adding BA solely at 0.5-3.0mg/L. There appeared bud redifferentiation only when appropriate concentration of combined BA,NAA and 2,4-D were applied.

结果表明：单独加入NAA0.5～3.0mg／L可诱导出绿色致密的愈伤组织，并再生出根，其中NAA0.5mg／L处理生根率最高，达90％；单独加入2，4-D0.1-3.0mg／L可诱导出白色疏松的愈伤组织；单独使用BA0.5—3.0mg／L不能诱导出愈伤组织；BA与NAA或2，4-D只有在适当的质量浓度范围内配合使用才能分化出芽，芽分化率最高的处理为BA3.0mg／L＋NAA0.5mg／L，分化率为30％。

The calibration curve of the SM2-Kit with standard SM2 inhibitor was typical sigmoid curve fitted to the four parameter logistic equation with the linear detection of 0.625 μg/L to 80.0 μg/L (R2=0.9911), the sensitivity of 0.9 μg/L, the IC50 of 5.82 μg/L and the detection limit of 1 μg/L. The recoveries of SM2 spiked in pig feed were 83.4 %, in pig urine were 89.5 %. The precision and accuracy of the assay as determined by inter-assay and intra-assay coefficient variation was both below 15 %. The SM2-Kit generally had 2.08 % cross-reactivity towards sulfamerazine and little or no cross-reactivity towards other sulfonamides. The dilution solution of SM2 had no effect on results of SM2-Kit. The validity of SM2-Kit in 4 ℃ was above six months.

研究结果表明，SM2-Kit的标准曲线呈典型的S型，相关系数R2=0.9911，符合4参数logit曲线拟合，线性检测范围为0.625 μg/L～80 μg/L，灵敏度为0.9 μg/L，半数抑制浓度(IC50)为5.82 μg/L，检测限为1 μg/L；饲料样、猪尿样的平均添加回收率分别为83.4 %、89.5 %，平均批内和批间变异系数均低于15 %；SM2-Kit与磺胺甲基嘧啶的交叉反应率为2.08 %，与其它磺胺类药几乎没有反应性；基质对SM2-Kit的检测结果影响不大；试剂盒在4 ℃可保存6个月。

And then adding NAA at low concentration in MS was used to give rise to roots. We also found that lower level of hormone could control effectively browninng and vitrifaction during the culture and G1 n (6mg/L) and AgNOj (2mg/L) supplemented in shoot induction media could improve the shoot information rates apparently (about90%).The whole period of plant regeneration from leaflets of peanut could be divided as five steps: germination - shoot induction -shoot elongation-rooting- tranplant.

用1／2MS培养基萌发花生种子，9-10d后，从无菌花生苗上切取幼嫩叶片中部为外植体。2500Lux光照和27±1℃条件下，在诱芽培养基(MS+BA3mg／L+NAA0.8mg／L+AgNO_32mg／L十Gln 6mg／L)培养12-14d即可观察到明显芽点或瘤状突起，较前人报道的培养时间大大缩短了，4w后芽点进一步发育成丛生芽，芽诱导率达90.2％，每个外植体平均产9个丛尘芽，然后转至培养基MS+BA3 mg／L+AgNO_32 mg／L上诱导芽的伸长，3-4w后可长至3-4cm，切下带有2-3片叶片的幼芽移至生根培养基(MS+NAA0.8mg／L+AgNO_32mg／L)，1w后切口处可见白色不定根形成。

Methods; Rat HSCs were treated with 0.4% fetal calf serum/DMEM for 48 hours and divided into the control group and experiment groups. HSCs in the control group were treated with 10% FCS, and HSCs in the experiment groups were treated with 10% FCS and low anticoagulative activity heparin with different concentrations (8 g/L, 40 g/L, 200 g/L, 1000 g/L, 5000 g/L). MTT reduction assay was used to evaluate the effect of low anticoagulative activity heparin on the growth of rat HSCs.

肝星状细胞经体积分数为0.4%胎牛血清／DMEM培养液同步化48h后分为对照组和实验组，对照组以体积分数为10%胎牛血清处理，实验组分别以体积分数为10%胎牛血清和不同质量浓度(8 g/L， 40 g/L， 200 g/L， 1000 g/L， 5000 g/L)低抗凝肝素处理，应用MTT法测定其对肝星状细胞生长的影响。

An optimized PCR reaction system for ISSR analysis was established:PCR was performed in a 20μl reaction mixture with 5μmol/L DNA, 2.5mmol/L Mg~(2+), 0.3mmol/Lof each dNTP, 300nmol/L primer, 1U Taq polymerase and 1×Buffer. Using 9 ISSRprimers,the genetic diversity among 23 Arrhenatherum elatius L.

3遗传多样性研究结果表明：通过模板DNA、引物、Mg~+、dNTPs、Taq酶五种ISSR反应体系的影响因素进行L_(16)(4~5)正交试验，建立了高燕麦草ISSR反应体系：20μl的反应体积中含有模板DNA 5μmol／L，引物300nmol／L，Mg~+2.5mmol／L，dNTP 0.3mmol／L，Taq酶1U及1×Buffer。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了