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Result: The nm-HAP-sol present the dispersed and uniformity needle. A431cells inhibition ratio was maximizing at the 5 day in the 400mg/L nm-HAP-sol group, the 200mg/L nm-HAP-sol group and the 100mg/L nm-HAP-sol group, inhibition ratio of the 400mg/L nm-HAP-sol group, the positive control group and the 50mg/L nm-HAP-sol group was respectively (77.89±6.29)%,(77.46±8.26)% and (1.23±0.15)%. It was detected that the number of A431 cells were persistently increasing in the 50mg/L nm-HAP-sol groups while decreasing in the 400mg/L nm-HAP-sol group, the positive control group and abidingly increasing for 4 and 3 days then decreasing in the 100mg/L and 200mg/L nm-HAP-sol group by inverted microscope.

结果：超声乳化法配制的HAP纳米溶胶呈均匀分散的针状；400mg/L、200mg/L和100mg/L溶胶组对A431细胞抑制率在第5天达到各组最大值，此时400mg/L溶胶组与阳性对照组的抑制率分别为（77.89±6.29）%和（77.46±8.26）%， 50mg/L溶胶组抑制率为（1.23±0.15）%；倒置显微镜下见50mg/L溶胶组细胞数目持续增加，聚集成簇，细胞呈现多角形或梭形，胞浆透亮，而100mg/L 和200mg/L溶胶组的细胞数目分别增加至第4天和第3天开始下降，400mg/L溶胶组和阳性对照组细胞数目持续减少，细胞体积缩小，漂浮细胞增多。

B Of the six basic media MS, MS1/2 (half-strength of MS salts and vitamins), WPM, DKW, B5 and SH, MS1/2 was the most proper one to induce somatic embryos. Somatic embryos generally regenerated directly from excised zygotic cotyledons. PGRs combination affected somatic embryogenesis significantly. Medium with NAA 1mg/L, TDZ 0.05mg/L, IBA 2—10mg/L combined with BA 10mg/L, or IBA 10mg/L integrated with BA 0-2mg/L gave the highest induction rate. Excised zygotic hypocotyls had the strongest potential to produce callus. Callus induction was also affected significantly by media and PGRs. The proper callus induction condition was MS1/2 medium containing NAA 1mg/L, IBA 10mg/L, BA 2-5mg/L and TDZ 0.05mg/L. Harvest period affect somatic embryogenesis significantly. Zygotic embryo explants collected from the end of July to the middle of August had strong potential to generate somatic embryos, when endosperm finished solidification, different parts of the embryos were completely formed, the size of embryos occupied about 2/3 of the embryo sac. Provided with optimized conditions, direct somatic embryogenesis rate can attain to 33. 68%, and callus induction rate of hypocotyls was up to 90.7%. Cytological observation on megasporogenesis and zygotic embryogenesis of Manchurian ash showed that the ovary was twicarpellum, twilocular with two ovules each loculus. The ovule was tenuinucellar and anatropous, with one megasporcocyte. The development of embryo sac is of the Polygoum type.

体细胞胚胎发生研究的结果表明：(1)成熟过程中的合子胚是诱导水曲柳体细胞胚胎发生的最佳外植体材料；(2)在所试验到的MS、MS1/2（将MS的所有成分均减半）、WPM、DKW、B〓、SH等六种基本培养基中，MS1/2是最适合诱导水曲柳体细胞胚胎发生的基本培养基；(3)水曲柳的体细胞胚胎发生以直接发生为主，体细胞胚主要来自于从合子胚分离的完整子叶；(4)培养基中的激素组合对水曲柳的体细胞胚胎发生有显著影响，诱导直接体细胞胚发生较好的激素组合有NAA 1mg/L+IBA 2，5，10mg/L+BA 10mg/L+TDZ 0.05mg/L和NAA 1mg/L+IBA 10mg/L+BA 0，2mg/L+TDZ 0.05mg/L；(5)合子胚分离的下胚轴具有最强的愈伤组织诱导潜力，少数愈伤组织可以分化出体细胞胚；(6)愈伤组织的诱导也受培养基和激素配比的显著影响，最适宜诱导的培养条件为MS1/2+NAA 1mg/L+IBA 10mg/L+BA 2，5mg/L+TDZ0.05mg/L；(7)采种时间对体细胞胚胎发生有显著影响。7月末到8月中旬的合子胚具有较强的体细胞胚发生潜力，此时种子尚未成熟，胚乳已呈固态，种胚的各个部分已分化完全，种胚体积占胚腔的大约2/3；(8)在各自综合的最适条件下，完整子叶的体细胞胚诱导率可达33.68%，下胚轴的愈伤组织诱导率可达90.7%。

The linear ranges of determination for pyrogallol, phloroglucinol, pyrocatechol, resorcinol and parodioxybenzene were 1.0×10〓～2.0 ×10〓mol l〓, 1.0×10〓～1.0×10〓mol l〓, 1.0×10〓～6.0×10〓mol l〓, 1.0 ×10〓～2.0×10〓mol l〓 and 1.0×10〓～6.0×10〓mol l〓 respectively, and their detection limits were 7.2×10〓mol l〓, 6.8×10〓mol l-1, 2.1 × 10〓mol l〓, 7.1×10〓 mol l〓 and 2.2×10〓mol l〓 respectively.

该方法测定连苯三酚、间苯三酚、邻苯二酚、间苯二酚和对苯二酚的线性范围分别为1.0×10〓～2.0×10〓mol l〓，1.0×10〓～1.0×10〓 mol l〓，1.0×10〓～6.0×10〓mol l〓，1.0×10〓～2.0×10〓mol l〓和1.0×10〓～6.0×10〓mol l〓；检出限分别为7.2×10〓mol l〓、6.8×10〓 mol l〓、2.1×10〓 mol l〓、7.1×10〓mol l〓和2.2×10〓mol l〓。

The results were showed that embryogenic calli were induced from young leaves, which were cultured on MS medium supplemented with 2,4-D 2mg/L and KT 0.2mg/L. For the proliferation of embryogenic calli, the suitable culture medium was MS+BA 8mg/L +NAA 1mg/L. The development and maturation of somatic embryo could be much improved by using the medium of MS+ZT 2mg/L or BA 5mg/L +IAA 0.2mg/L. For the induction of secondary somatic embryo from integral somatic embryo, the suitable culture medium was MS+KT 0.1mg/L+NAA 0.01 mg/L or MS+ZT 0.1mg/L+NAA 0.01 mg/L, the proliferation frequency is 214%, 256% respectively. The cotyledonary generated from somatic embryos of Aesculus hippocastanum L.

本文就欧洲七叶树的组织培养和体细胞胚发生以及植株再生进行了系统研究，主要结果如下：以植物细胞具有全能性的理论为依据，以欧洲七叶树幼嫩叶片为外植体，进行体细胞胚胎发生研究，研究结果表明，诱导愈伤组织的适宜培养基是MS+2，4-D 2mg／L+KT0.2mg／L，MS+BA 8mg／L+NAA 1mg／L有利于胚性愈伤组织的诱导和增殖，添加ZT 2mg／L或BA 5mg／L和IAA 0.2mg／L的MS培养基有利于体细胞胚发育和成熟，体细胞胚可直接诱导次生胚发生，MS+KT 0.1mg／L+NAA 0.01 mg／L或MS+ZT 0.1mg／L+NAA 0.01 mg／L培养基诱导效果最好，增殖频率分别为214％、256％。

For the bud directly differentiation , the suitable culture medium was MS+BA 2mg/L +NAA 0.2mg/L or MS+TDZ0.01mg/L +NAA0.2 mg/L, of which the induction rate was 88% and 100% respectively. An efficient tissue culture system has been developed with the bud of mature seed of Aesculus hippocastanum L. as explants . Buds were induced from 2cm high young plantlet cultured on MS medium supplemented with 0.6 mg/L 6-BA plus 0.1mg/L or 0.4~0.6mg/L ZT plus 0.1 mg/L NAA for 15 d, and the induction rate was 100%, the mean No. of buds was 35.7; The combination of MS+0.2mg/L 6-BA +0.1mg/L NAA + 10mg/L AD was the suitable culture medium for elongation of the buds.

以欧洲七叶树成熟种子的胚芽进行离体培养和快速繁殖，结果表明：高约2cm的无菌苗在MS+0.6mg／L 6-BA+0.1 mg／L NAA或MS+0.4～0.6mg／L ZT+0.1 mg／L NAA培养基上培养15 d左右可诱导出不定芽，分化频率为100％，平均每株产芽35.7个；MS+0.2mg／L 6-BA+0.1mg／L NAA+10mg／L AD培养基有利于芽伸长；生根培养基为1／2MS+0.4 mg／L NAA+0.2mg／L IBA时，生根率可达75％。

A boiler water controlling system with basifier a mg/L Na3PO4+b mg/L NH3+c mg/L NaOH is investigate in the paper. Several calculate methods are discussed and the precision pH value calculating equation is proposed for a cycle chemistry system that has seven changing parameters coexisting together, i. e. a mg/L Na3PO4+b mg/L NH3+c mg/L NaOH+d mg/L H2SO4+e mg/L CH3COOH+f mg/L CO2+g mg/L SiO2. As an example of equilibrium phosphate treatment, the pH value are obtained and showed in graphics with the four kinds of impurity changing from 0 to 1.0 mg/L. The calculating results can be used in practical boiler water pH value control.

对于一个采用a mg/L Na3PO4+b mg/L NH3+c mg/L NaoH碱化剂的炉水控制体系，主要讨论在炉水中存在d mg/L H2SO4+e mg/L CH3COOH+f mg/L CO2+g mg/ L SiO2 4种杂质时炉水pH值的计算方法，得到通用的pH值计算表达式；并以炉水平衡磷酸盐处理工况为例，计算这4种杂质在0-1 mg/L变化时，其单独或联合作用对炉水pH值的具体影响程度，以6幅图形展示计算结果，该结果可应用于炉水pH值的实际控制。

This paper presents a study of Eucalyptus dunnii tissue culture, using its seeds as explants. The results reveal that MS medium is a suitable basic medium for its seeds to germinate and grow; that MS+KT1.0 mg/L+2, 4-D2.0 mg/L+Homocysteine30mg/L is a good medium to induce its seeds to dedifferentiate directly into callus; that H+6-BA2.0 mg/L+IAA0.2 mg/L is a good medium to make its bud on the seedling reproduce more buds; that MS+6-BA2.0 mg/L+NAA0.2 mg/L is a better medium to induce its root on the seedling into callus; that B5+6-BA2.0 mg/L+2, 4-D0.2 mg/L can induce its under-hypocotyl to differentiate into buds; and that B5+Ad2.0 mg/L+IAA0.2 mg/L can induce its under-hypocotyl into callus to generate normal buds.

以邓恩桉种子为外植体，探讨用最少种子快速繁殖最多幼苗的方法，结果表明：MS培养基是较适合邓恩桉种子萌芽和生长的基本培养基；诱导种子直接脱分化成愈伤组织的较佳培养基配方为MS+KT1.0 mg/L+2，4-D2.0 mg/L+半胱氨酸30 mg/L；以邓恩桉实生苗的芽来繁芽的较理想培养基配方为H+BA2.0 mg/L+IAA0.2 mg/L；邓恩桉实生苗的根诱导愈伤组织的较佳培养基配方为MS+6-BA2.0 mg/L+NAA0.2 mg/L；诱导邓恩桉下胚轴分化芽较佳培养基配方为B5+6-BA2.0 mg/L+2，4-D0.2 mg/L；诱导邓恩桉下胚轴脱分化为胚性愈伤组织的较佳培养基配方为B5+Ad2.0 mg/L+IAA0.2 mg/L。

The effects of solution\'s pH value, mol ratio between phenyl aldehyde and L-Arginine, concentrations of sodium chloride, ammonia chloride andL-Lystine, initial concentration of L-Arginine on precipitation rate of a -tolylene arginine were investigated, the results indicated that phenyl aldehyde precipitation method\'s appropriate pH value was higher than 11, suitable mol ratio between phenyl aldehyde and L-Arginine was 1.25, initial concentration of L-Arginine was must higher than 15g/L, sodium chloride had little effect on precipitation rate, on the contrary, ammonia chloride and L-Lystine had great effect on it, both of them must be eliminated; At the same time, adsorption isotherm at 25 C of L-Lystine on anion exchange resin A was measured, the result showed that the maximal equilibrium adsorbance was 30mg/g, the influences of solution\'s pH value, temperature, concentration of chloride ion on adsorption of L-Lystine by anion exchange resin A were also studied, the results indicated that appropriate operational pH value was 0.5 0.2, the effect of temperature on adsorption ratio was little, process could be operated at room temperature, the concentration of chloride ion must be eliminated.

考察了溶液pH值、苯甲醛与L-精氨酸摩尔比、氯化钠与氯化铵浓度、L-赖氨酸浓度、L-精氨酸初始浓度对苯甲醛沉淀L-精氨酸的影响，结果表明，沉淀反应的适宜pH值大于11，苯甲醛与L-精氨酸的适宜摩尔比为1.25，(来源：Aa6BC论文网www.abclunwen.com)L-精氨酸适宜的初始浓度在15g/L以上，氯化钠的存在对沉淀率基本上没有影响，而氯化铵和L-赖氨酸的存在使沉淀率下降；同时测定了25℃时阴离子交换树脂A吸附L-赖氨酸的吸附等温线，表明其最大平衡吸附量为30mg/g，考察了溶液pH值、温度、氯离子浓度对阴离子交换树脂A吸附脱除L-赖氨酸的影响，结果显示从L-精氨酸和L-赖氨酸混合液中吸附分离出L-赖氨酸的适宜pH值为10.5±0.2，温度对L-赖氨酸吸附率影响不大，吸附过程可在室温下进行，氯离子的存在使L-赖氨酸的吸附率降低。

L~(-1) each, 3.5μL of downstream primer 10μmol ? L~(-1,4μL of RNA,5μL of double-distilled DEPC H_2O;PCR mixture consisted of 20μL of RT-Mixture,5μL of 10 × PCR buffers, 1μL of Taq5U·μL~(-1, 5uL of mixture of dNTPs the 2.5 mmol ?

包括AMV5u·μL~(-11μL，5×Reverse transcriptase buffer 4μL，RNase Inhibitor40U·μL~(-10.5μL，dNTPs混合物10mmol·L~(-1each2μL，下游引物10μmol·L~(-13.5μL，模板4μL，水5μL；PCR反应体系：总反应体系为50μL。

The research on the tissue culture and cell suspension culture showed that thesuitable culture medium for inducing bud was MS supplemented with 1.5mg/L 6-BAand 0.1mg/L NAA, and for the generation-continuing multiplication was MSsupplemented with 1.5mg/L 6-BA and 0.2mg/L NAA. The rooting medium was1/2MS supplemented with 0.5mg/L IBA and the rooting rate was 45.0%. Plantletsurvival after transfer to sand was 52.5%.The induction rate of calli was66.7%～86.6% and the optimum medium was MS medium with 0.5mg/L 6-BA and2.0mg/L 2,4-D. The calli became smallest partical size, friable and had gooddispersion ability after 3 times successive transfer culture, the optimum medium wasMS medium with 0.2mg/L 6-BA and 2.0mg/L 2,4-D. Culturing these particles on sixkinds of MS liquid media with different hormone contents, the optimum medium wasselected basing on he change of the density of single-cell, the density of cellaggrefate and the mass of cell.

对蒜头果进行的组织培养与细胞悬浮培养研究结果表明：MS+6-BA1.5mg／L+NAA0.1mg／L+蔗糖3％激素组合能够较好地诱导芽的初始分化和增殖，适宜的芽苗继代增殖培养基为MS+6-BA1.5mg／L+NAA0.2mg／L+蔗糖3％；采用1／2MS+IBA0.5mg／L+蔗糖3％为生根培养基，生根率为45.0％；移栽到河沙的生根苗成活率为52.5％；愈伤组织诱导率为66.7％～86.6％，其中以MS+6-BA0.5mg／L+2，4-D2.0mg／L+蔗糖3％的培养基最佳，其诱导出的愈伤组织具有较强的增殖能力和较好的脆散结构，最佳继代培养基为MS+6-BA0.2mg／L+2，4-D2.0mg／L+蔗糖3％，且培养基中的6-BA与2，4-D浓度的比值越小，愈伤组织生长越快，结构越脆散，增殖率越高；将继代后的愈伤组织转入6种含不同激素浓度组合的MS液体培养基中进行振荡培养，在综合分析各培养基的单细胞密度，细胞团块密度，细胞生物量增长率等指标后，初步筛选出MS+6-BA0.2mg／L+2，4-D2.0mg／L培养基为较好的液体培养基。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了