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In batch cultures, glucose, xylose and arabinose in cornhusk dilute acid hydrolysate after detoxification were consumed by Actinobacillus succinogenes NJ113, when the total sugar concentration was 50 g/L in medium, the final succinic acid concentration of 34.2 g/L was obtained, resulting in a succinic acid yield of 0.68 g/g and a succinic acid productivity of 0.83 g/. When the total sugar concentration was 68.2 g/L in medium, the succinic acid concentration was 42.3g/L, the yield was 0.62g/g, and productivity was 0.98 g/.

经过脱毒处理后，产琥珀酸放线杆菌 NJ113均能利用水解液中的葡萄糖、木糖、阿拉伯糖，培养基总糖浓度为50 g/L时，丁二酸分批发酵的质量收率可达0.68 g/g，浓度可达34.2 g/L，生产强度达0.83 g/，总糖浓度为68.2 g/L时，丁二酸质量收率仍可达0.62 g/g，浓度42.3 g/L，生产强度0.98 g/。

Every explant was pricked with a dissector at the center of one subregion before inoculated on MS+BA 1mg/L+NAA 4mg/L+2,4D 05mg/L medium and incubated in darkness for 7 days,then transfered to MS+BA 1mg/L medium.85% of the explants regenerated somatic embryos directly around the prick after 40 days.

每一叶片在1个亚区中心部位用解剖针刺伤1点后接种于MS＋BA1mg／L＋NAA4mg／L＋2，4D05mg／L＋蔗糖20g／L的培养基上，暗培养7d后，转接到MS＋BA1mg／L＋蔗糖20g／L的培养基上继续暗培养，40d后，85％的叶片在刺伤口周围发生直接类型体细胞胚胎。

The ideal activation process parameter as follow: The nickel sulfate hexahydrate concentration 15g·L~(-1);The Sodium borohydride concentration 15g·L~(-1); The Sodium hydroxide concentration 10-15 g·L~(-1);Hydrochloric acid concentration 10-15 g·L~(-1);The activation time in A solution 9min, the activation time in B solution 90s.

理想的活化工艺参数为：硫酸镍15g·L~(-1)，硼氢化钠15g·L~(-1)，氢氧化钠10-15 g·L~(-1)，盐酸10-15 g·L~(-1)，A液中活化的时间控制在9min左右为最佳，B液中活化的最佳时间为90s。

Shanghai Kyowa Amino Acid Co., Ltd. is a Sino-Japan joint venture found ed by Guanshengyuan Co.,Ltd and Kyowa Hakko Kogyo Co.,Ltd.

现公司最新供应以下产品： L-丙氨酸、L-精氨酸、L-盐酸精氨酸、L-门冬氨酸、L-谷氨酸、L-组氨酸、L-盐酸组氨酸、L-盐酸赖氨酸、L-脯氨酸、L-丝氨酸、L-苏氨酸等。

It turned out reproduction rete of chrysanthemum was raised,the regular,non-plant diseases and insect pests could be offered to the market. Shoot tip were used as explants to study the effects of different sterilization methods, illumination intensity and concentrations of 6-BA and NAA on shoot propagation. Rooting with perlite, vermiculite and river sand instead of agar was studied. In the process of preliminary multiplication, the effect of using 0.1% HgCl_2 in sterilization of explants was better than using saturated bleaching solution. The highest multiplication rate was found under whole illumination. MS media with 6-BA from 0.2 mg·L~(-1) to 5 mg·L~(-1) and NAA from 0.05 mg·L~(-1) to 2 mg·L~(-1) were tested and as a result, the highest differentiation rate and the multiplication rate was reached with 0.2 mg·L~(-1) 6-BA, 0.05 mg·L~(-1) NAA.

本实验以菊花的茎尖为外植体，研究了不同灭菌方法，光照强度，全光照、半光照和弱光处理以及不同6-BA和NAA浓度及配比对于试管苗增殖的影响，并研究了珍珠岩、蛭石和河砂等琼脂替代物对于菊花生根的影响，结果表明：在初代培养物建立的过程中采用0.1％升汞进行表面灭菌的效果好于饱和漂白粉的灭菌效果；在全光照条件下外植体的增殖倍数最高；在试管苗增殖培养的过程中，以MS为基本培养基，并在培养基中添加0.2～5mg·L~(-1)6-BA和0.05～2mg·L~(-1)NAA，其中0.2mg·L~(-1)6-BA和0.05mg·L~(-1)NAA最适于外植体的分化和增殖，其分化率为100％，增殖倍数为12。

Results were shown as followings:(1) Sodium selenite at 0～2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5～2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下：（1）0～2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力；而对SMMC-7721细胞的作用不显著；（2）该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度；而对SMMC-7721细胞的作用均不显著；（3）高浓度硒（5μmol/L以上）显著性抑制L-02细胞生长、致细胞过氧化，灯盏花素能拮抗硒所致过氧化、降低硒毒性；（4）0.5～2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧，同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著；（5）富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白，其结构和功能有待研究；（6）通过已有的含hTERT基因质粒，成功构建hTERT反义RNA表达质粒，转染SMMC-7721细胞后对其生长具有抑制作用。

Methods Six adult Wistar rats were randomly divided into two groups: group A (n=3) and group B (n=3). 2μl volume of 0.8mmol/L and that of 1.6mmol/L aqueous solution of MnCl2 was injected into the vitreous body of the left eye of group A and group B respectively.

6只Wister大鼠，随机分为两组，每组3只，一组左侧眼球玻璃体内注射0.8mmol/L MnCl2 2μl，一组左侧眼球玻璃体内注射1.6mmol/L MnCl2 2μl，用1.5T超导磁共振扫描，观察视觉传导通路的显示；一周后6只大鼠右侧外侧膝状体注射1mmol/L MnCl2 1μl，观察视觉皮层的定位。

I am most dear L You took me into the seat A Midsummer Night's Dream Only you A pupil of the eye and smile all the world to do with desolation Bright also move the entire hall Is the memory of that A bow Zhang Yi Tai He Yan - I thought It was alleged in the A smile A frown A Diyin A shallow sing A little bit of mottled shadow, for some of the yellowing paragraph in the past Block is a dump destroyed the city wall Or a tie game time This has been like to go on This has been watching you L As with all of the time became the music of the 12 Piaofei In this letter, Play the most beautiful movement All of the notes, where solidification In the slender your fingertips In your hair soft, In it you tired yet bright vision In it you take it lightly but a lot of sincere hand My dear L You see, The accumulation of sweets on the table is not less than the touch heaven A person behind it is not always empty picture At the end of the world it really does not cross the wall in the past In the end it is only a direction too far The sorrow of so many suffering So much of a loss Dihui After all, only in the past of injury Dear I promise After a good smile No longer sad

我最最亲爱的L 是你带我走进了这席仲夏夜之梦也唯有你一眸一笑都带尽了人世苍凉一举一动亦明亮了整个厅堂是记忆中的那一低头一抬掌一阖眼一思量还是那臆想中的一微笑一皱眉一低吟一浅唱一点点斑驳的影子，一段段泛黄的过往是一座座倾圮的城墙抑或是一场场饕餮的时光想这样一直下去一直这样看着你 L 如同所有时光化成了十二张飘飞的乐谱在这个字母上奏出了最华丽的乐章所有的音符凝固在那里在你那修长的指尖在你那柔软的发梢在你那疲惫而不失明亮的眼光在你那淡然却不少真挚的手掌我亲爱的L 你看桌上堆积的甜食并非是触及不到的天堂一个人的背后也并非总是空荡荡的景象世界的尽头是否真的有跨不过去的高墙那终究只是一个太远的方向那么多的悲哀痛苦那么多的低回彷徨终究都只是过往的伤亲爱的答应我以后好好微笑不再悲伤。。。。。

Pure magnesium was treated by A. C. treatment technique in 0.25mol/L NaZSO4+0.1mol/L NAOH solution. The surface morphology was observed by scanning electron microscopy. The contact angle of the treated surface of pure Mg was measured by the First Ten Angstroms instrument and then its surface free energy can be calculated. The effect of alternating current treatment on the corrosion resistance of the pure magnesium was studied through electrochemical methods in 0.05mol/L NACI+0.01mol/L NAOH solution. The experimental results showed that a uniform and perfect oxide film was formed after A.

采用载波处理方法，用0.25mol/L Na2SO4+0.1mol/L NAOH作为载波处理溶液，对纯镁试样进行载波处理，通过正交试验确定载波处理的最佳工艺参数；用扫描电了显微镜观察载波处理后试样的表面形貌；用First Ten Angstroms仪器测量蒸馏水纯镁的接触角并计算出表面能；用电化学方法研究了在0.05 mol/L NACI+0.01mol/L NAOH溶液中载波处理对纯镁耐蚀性能的影响。

Mmol/L to juvenile Japanese sea bass, and 75.86, 63.46, 56.42 mmol/L to black rockfish. When pH is steady at 8.40 + 0.10, the 24-, 48-, 96-hour LC50 of Alk are 72. 79, 65. 28, 57. 92 mmol/L to Japanese sea bass, and 69. 47, 59. 37, 51. 96mmol/L to black rockfish. It is obvious that the toxicity of Alk to fish increases with the progressive increase of Alk or time when pH is at a constant level.3. According to analysis of variance of orthogonal test, pH and Alk have signification effects on the mortality rate of black rockfish.

pH在正常养殖范围时，通过碱度对海水鱼类鲈鱼、黑鲪的急性毒性试验，得到pH=8.10±0.10时，碱度对鲈鱼稚鱼(体长1.6～2.2cm)24、48、96h的半致死值为60.88、44.36、36.06mmol／L，安全浓度为7.07mmol／L；pH=8.05±0.10时，碱度对黑鲳幼鱼24、48、96h的半致死值为75.86、63.46、56.42mmol／L，安全浓度为13.32mmol／L.pH=8.40±0.10时，碱度对鲈鱼幼鱼24、48、96小时的半致死值为72.79、65.28、57.92mmol／L，安全浓度为15.76mmol／L；对黑鲪幼鱼24、48、96h的半致死值为69.47、59.37、51.96mmol／L，安全浓度13.01mmol／L。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。