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To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

Little eight-year accumulation and unremitting efforts, today's Hongyuan formed a strong production, sales and service network systems integration services, mainly embodied in: l, in Kunming, Sichuan, set up a fresh Lingyuan cut-flower production base, roses, lilies, tulips Series Fists are my company's products; 2, and Guangzhou into the United States, Bo Park, Tahan, a pioneer in high-tech and Hainan Nongda set up a long-term cooperative relations, ensuring product quality and stability sexual; 3, in Beijing Chaoyang District, Tongzhou District, and has set up specialized miniata production and storage of tropical flowers and trees pin base, engaged in viewing tolerant plants, potted plant, bonsai, seasonal flowers and green seedlings and other characteristics of tropical flowers and trees and landscape gardening equipment wholesale leasing operations; 4, in the Haidian District÷ìset up a flower production base of the flower can meet customer demand; 5, in Beijing too flowers Levin Office, Office of, Tung Ha, Xinglong, Kunming and other flowers of Dounan market standing distribution point; the Company with state-owned buildings, Yinhua Fund, Beiqi Futian, Thunis network than Granville, 31 heavy machine Oriental Electronics, Dentsu advertising, white ads, the first steam shares Bluebird fitness, Hao Sha Fitness, Siemens, the Three Gorges, the Capital Airport, light Xin Hotel, Sinopec, assembly, Shanghai city, China Hai-cheng, the Ministry of Agriculture and the Ministry of Agriculture office kindergarten organs, Beijing Publishing Group II°àunits such as multi-year cooperation provided×°flowers, flowers and garden maintenance engineering courier service, received by customers."Customer-base to the quality of survival, in good faith and development" is our company goal.

八年的点滴积累与不懈努力，今天的鸿源形成了一个强大的集生产、销售、服务为一体的网络式系统服务，其主要体现在：l、公司在昆明、四川、凌源建立了鲜切花生产基地，玫瑰、百合、郁金香系列是我公司的拳头产品；2、与广州的成美、波园、大汉、先锋、高新及海南的农大建立了长期合作关系，确保了产品的质量及稳定性；3、在北京通州区和朝阳区已建立专业化君子兰生产和贮销热带花木基地，从事观赏耐荫植物、盆花、盆景，应季花卉及绿化苗木等热带特色花木及园林园艺器材的批发租赁业务； 4、在海淀区西三旗建立了草花生产基地，能够满足客户对草花的需求；5、在北京的莱太鲜花厅、绿植厅、通厦、兴隆、昆明的斗南等花卉市场常设经销点；本公司与国企大厦、银华基金、北汽福田、清华紫光比威网络、三一重机、东方电子、电通广告、白马广告、首汽股份、青鸟健身、浩沙健身、西门子、长江三峡、首都机场、轻鑫宾馆、中国石化、总装、上海大都会、中国海诚、农业部办公楼及农业部机关幼儿园、北京出版集团等二百余家单位多年合作，为其提供的花卉租摆、鲜花速递及园林养护工程服务，深受客户好评。

A connector is an injection molding from the positioning kits and installed in the positioning of the Inner-Block, packaged in the seat of the spring and reed composition, described the position set of cylindrical Chengyuan, the location of the hole sets A circular from the convex to capacity at the first spring home district and the Department of card system for the second district of the blocks in the positioning of the sets of holes in the first referred to the Ministry, since the port inside the shaft Two symmetrical to the hole within the targeted slot, a connector and adapter combination of positioning, positioning slot in the rear, the agreement sets in the central part of setting the level of L-shaped slots, so that the connector and adapter reliable combination of the Practical use of new high-strength engineering materials first injection molding, greatly simplified the process, a connector improve the accuracy of the positioning trough of the utility model to improve, even if the products improve the safety and reliability, and more convenient assembly, product structure is simple And optimize the manufacturing process, reducing costs and improve productivity.

一种连接器是由注塑一次成型的定位套和安装在定位套内的机座、套装在机座上的弹簧和簧片组成，所述的定位套呈圆柱状，该定位套的内孔由一内凸圆隔出容置弹簧的第一区部和卡制机座的第二区部，在定位套的内孔，位于所述的第一区部处，自端口向内，轴向对称设置两条内孔定位槽，起到连接器与适配器结合的定位作用，在该定位槽尾部，约定位套的中部设置L形水平槽孔，使连接器与适配器可靠结合；本实用新型采用高强度工程材料一次注塑成型，大大简化了加工过程，提高了一种连接器的精确度，本实用新型定位槽的改进，即使产品安全可靠性提高，又更方便组装，产品结构简单、制造工艺优化，从而降低了成本，提高了生产率。

In this paper, we mainly use complex semisimple algebras knowledge. First, we give the structure formula of C. Second, we show that any n x n symmetric matrix A is congruent under the symplectic group to the direct sum of a identity matrix and a tridiagonal matrix.

记正交代数和辛代数的交集构成的李代数为L，本文主要是运用复半单李代数的知识，首先给出了L的结构公式，然后给出了任一n×n对称矩阵A辛合同于一个单位矩阵和一个三对角矩阵的直和。

Introducing the notions of module algebra, Smash product and left integral we consider the relation between A#L and invariant subalgebra A〓.

在引入模代数、Smash积、左积分的定义之后，我们考虑A#L与不变子代数A〓之间的关系。

This is the so-called Duerlite algorithms: from the bottom row of the matrix A do elementary transformation, the diagonal elements into the bottom left of zero, and then show the effect of these lines is equivalent to transform the left by a unit lower triangular matrix, this series of unit lower triangular matrix inverse is the product of L matrices, it is also a unit lower triangular matrix.

这正是所谓的杜尔里特算法：从下至上地对矩阵A做初等行变换，将对角线左下方的元素变成零，然后再证明这些行变换的效果等同于左乘一系列单位下三角矩阵，这一系列单位下三角矩阵的乘积的逆就是L矩阵，它也是一个单位下三角矩阵。

In this section, we also give a conjecture: A finite lattice is isomorphic to the lattice of flats of a finite poset matroid if and only if it is a semi-modular lattice.

本节的一个中心问题是提出了一个猜想：一个有限格L同构于一个有限偏序集拟阵的闭集所构成的格当且仅当L是一个半模格。

The effect of progesterone could be potentiated by GABA A receptor agonist muscimol and antagonized by GABA A receptor antagonists bicuculline and picrotoxin as well as GABA B receptor agonist baclofen. The effect of progesterone and the actions of these drugs mainly manifested in the concentration range of progesterone 0 01～0 05 μmol/L.

该作用可被氨基丁酸A受体激动剂蝇蕈醇加强，被氨基丁酸A受体拮抗剂荷包牡丹碱和印防己毒素以及氨基丁酸B受体激动剂巴氯芬阻断，而且孕酮的效应以及各药物对其的影响均集中在0.01～0.05 μmol/L孕酮浓度中较为明显。

Were the only alive one but really flaring and following a fast medical evacuation in my country in AMERICA, and 13 days of unemployment which I had remained and my reveille J had already a part of my body brulée and paralized with left hand and really I had lost all my memory but God thank you this grace day has L evolution of medicine and of the réaducations which I underwent I found myself a little but J always have a part of my body flaring.

是唯一还活著，但真正燃烧和下面的一个快速医疗后送我国在美国， 13天的失业问题，我一直和我起床生已经成为我身体的一部分brulée和paralized与左手实在我失去了我所有的记忆，但是上帝的恩典感谢你这一天L演变医药和réaducations我经历了我发现自己一点，但总是有J的一部分，我的身体燃烧。

The variation of Rg for a flexible chain shows a tilted-L-shaped Rg curve. That for a semiflexible chain is characterized by a U-shaped curve. A stiff chain exhibits an almost straight Rg curve.

易弯曲链的尺寸随盐浓度变化曲线呈现了一个类似向左倾斜的L字型的曲线，半易弯曲鍊的尺寸随盐浓度变化曲线呈现了一个类似U字型的曲线，而刚硬链的尺寸随盐浓度变化曲线则是一条近乎水平的直线。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。