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It has been indicated that Yw18 could utilize methylparathion and ρ-nitrophenol,a main metabolite product for the insecticide,as sole source of carbon and it was identified as Ochrobacterum sp,and capable of degrading 50 mg·L-1 of methylparathion,ρ-nitrophenol,rapidly in 0.5 h and 8 h respectively,after analyzing metabolite products with ga...

用气相色谱法和分光光度法对Yw18的降解性能进行了研究，结果表明，在0.5 h内它对50 mg.L-1甲基对硫磷的降解率达90%以上，在8 h内能将50 mg.L-1对硝基苯酚完全降解。对该菌进行了系统发育研究，并用PCR法克隆其甲基对硫磷降解酶基因mpd。

Time constants do not increase but the weights of the fittings concentration de pendently enhance as the external Ba2+ concentration augments. So the inac tivati on becomes faster and faster as the external Ba2+ concentration increases. Time constants decrease and the weights of the fittings concentration dependently enh ance as the external Ba2+ concentration augments when external Ba2+ concentratio n (10 or 100 μmol/L) is higher. So the inactivation becomes faster and faster. It is showed that Ba2+ can go deeper in IRK1-T from the surface of the ch annel a s external Ba2+ concentration increases.

三级指数拟合表明：细胞外Ba2+低浓度(1和3 μmol/L)时，Ba2+与K+相互竞争同一结合位点，随着Ba2+浓度的增加，时间常数不增加但拟合的权数却浓度依赖性增加，所以失活过程随Ba2+浓度的增加越来越快；细胞外Ba 2+高浓度(10和100 μmol/L)时，时间常数随Ba2+浓度的增加而减少，拟合的权数却浓度依赖性减少，失活过程也越来越快，说明Ba2+作用位点由通道的表面部位进入通道更深的地方。

Time constants do not increase but the weights of the fittings concentration de pendently enhance as the external Ba2+ concentration augments. So the inac tivati on becomes faster and faster as the external Ba2+ concentration increases. Time constants decrease and the weights of the fittings concentration dependently enh ance as the external Ba2+ concentration augments when external Ba2+ concentratio n (10 or 100 μmol/L) is higher. So the inactivation becomes faster and faster. It is showed that Ba2+ can go deeper in IRK1-T from the surface of the ch annel a s external Ba2+ concentration increases. CONCLUSION: Two diffe rent mechanisms for external Ba2+ blocking exist.

三级指数拟合表明：细胞外Ba2+低浓度(1和3 μmol/L)时，Ba2+与K+相互竞争同一结合位点，随着Ba2+浓度的增加，时间常数不增加但拟合的权数却浓度依赖性增加，所以失活过程随Ba2+浓度的增加越来越快；细胞外Ba2+高浓度(10和100 μmol/L)时，时间常数随Ba2+浓度的增加而减少，拟合的权数却浓度依赖性减少，失活过程也越来越快，说明Ba2+作用位点由通道的表面部位进入通道更深的地方。

Time constants do not increase but the weights of the fittings concentration de pendently enhance as the external Ba2+ concentration augments. So the inac tivati on becomes faster and faster as the external Ba2+ concentration increases. Time constants decrease and the weights of the fittings concentration dependently enh ance as the external Ba2+ concentration augments when external Ba2+ concentratio n (10 or 100 μmol/L) is higher. So the inactivation becomes faster and faster. It is showed that Ba2+ can go deeper in IRK1-T from the surface of the ch annel a s external Ba2+ concentration increases. CONCLUSION: Two diffe rent mechanisms for external Ba2+ blocking exist.

三级指数拟合表明：情绪细胞外Ba2+低浓度(1和3 μmol/L)时，Ba2+与K+相互竞争同一结合位点，随着Ba2+浓度的增加，时间常数不增加但拟合的权数却浓度依赖性增加，所以失活过程随Ba2+浓度的增加越来越快；情绪细胞外Ba2+高浓度(10和100 μmol/L)时，时间常数随Ba2+浓度的增加而减少，拟合的权数却浓度依赖性减少，失活过程也越来越快，说明Ba2+作用位点由通道的表面部位进入通道更深的地方。

The effects of variety preculture time, activate medium of A.tum, concentration of Ca, inoculation time, treated with AgNO3 and PVP or not, delay selection time on transformation frequency were studied as well. The results demonstrated that using 20Omg.LCef, 8 mg.L1Kam.

结果表明，使用200mg.L~(-1)Cef、8mg.L~(-1)Kam、预培养2天、使用GCJ8活化培养基、在预培养和共培养基中使用5倍Ca~离子浓度、侵染1分钟、使用4mg.L~(-1)AgNO3和0.7％PVP，延迟筛选5天时取得了最高的转化频率。

Results EROD activities in liver of wild crucian from Zhangtan, Jiangjunyan, Fuyang and Wenyan (23.51±4.17, 16.79±7.39, 18.74±5.16, 18.65±8.86 nmol/) were significantly higher than that of Jiekou (7.84±2.42 nmol/g promin), and no difference was observed between the other sites and Jiekou. Three dose groups (0.25, 0.50, 1.00L/dish water sample extracts) were used in Ames test. Reversional mutation rate of TA98 colony exposed to extracts from Zhangtan was 2.21, 3.39, 6.95 without S9, and 2.70, 4.07, 6.23 with S9, respectively. The revesional mutation of TA100 colony was 2.55, 2.81, 5.36 without S9, and 1.73, 2.86, 4.14 with S9, respectively. Reversional mutation rate of TA98 colony exposed to 1.00L/dish extracts from Jiangjunyan with S9 was 2.22, and there was a dose-response relationship in reversional colony count. Ames tests of the other extracts were negative.

结果 樟潭、将军岩、富阳、闻堰点位野生鲫鱼肝EROD酶活性分别为(23.51±4.17)，(16.79±7.39)，(18.74±5.16)，(18.65±8.86)nmol/，明显高于街口[(7.84±2.42)nmol/]，其他点位与对照比较差异无统计学意义；樟潭水样浓集物在浓度为0.25，0.50，1.00 L/皿时在不加与加S9条件下，TA98回变菌落突变率MR值分别为2.21，3.39，6.95，2.70，4.07，6.23；TA100回变菌落MR值分别为2.55，2.81，5.36，1.73，2.86，4.14；Ames试验结果为明显阳性；将军岩水样浓集物在浓度为1.00 L/皿且加S9条件下TA98MR值为2.22，且在浓度为0.25，0.50，1.00 L/皿加S9条件下TA98回变菌落数呈剂量－反应关系，MR值分别为0.88，1.98，2.22；Ames试验结果为弱阳性；其他点位均为阴性。

L-NMMA can reduce the damage of contralateral testis of prepubertal rats with unilateral testicular torsion during the early period, and L-NMMA may also have a protective effect on the testicular spermatogenetic function in the later stage.

L-NMMA可以减轻大鼠单侧睾丸扭转后对侧睾丸的早期损害，对晚期对侧睾丸的生精功能具有一定的保护作用。

The results are unsure for experimental use;The rabbit's corneas that were removed with upper-half of corneal limbal epithelium lamella and erased the center corneal epitheliums were transparent with intact corneal epithelium;In the approach,the corneal and limbal epitheliums were burned with a cotton swab socked in 1 mol/L NaOH,there were 4 rabbits' corneal stroma happened perforation or ulcer and symblepharon,and the other one presented corneal epithelium phenotype.This is an applicable method to create the pathological model of corneal limbal stem cell total deficiency.

结果表明，处理后4周，全周角膜缘上皮板层手术切除，中央角膜上皮层用1 mol/L NaOH擦除的5只试验家兔角膜表面全部血管化、结膜化，未发生睑球粘连，角膜基质胶原纤维完整未见溃疡、穿孔等病变，细胞印迹学检查为结膜表型，可作为实验性角膜缘干细胞移植的病理模型；全周角膜缘上皮板层手术切除，中央角膜上皮用生理盐水擦除的5只试验家兔，有2只为结膜表型，另3只为角膜表型，观察期内结果不稳定；半周角膜缘上皮板层手术切除，中央角膜上皮层用生理盐水擦除的5只试验家兔，角膜表面透明，全部为角膜表型；直接用1 mol/L NaOH擦除角膜缘和中央角膜上皮的试验家兔，有4只角膜基质胶原纤维断裂、溶解，并伴有严重的溃疡、穿孔、睑球粘连等病变，不能用于移植试验，另1只角膜表面透明，未见结膜和新生血管长入，细胞印迹学检查为角膜表型。

The incidence of abnormal lipid trigone in children with a family history of premature CHD or non premature CHD and the controls were 52.6%, 10.9% and 1.2%, respectively.

有早发CHD家族史者LDL-C/HDL-C(2.85±1.21)高于无早发家族史组(1.37±1.11)和对照组(0.96±0.68)(P均。01)，HDL-C水平［(1.11±0.26) mmol/L］低于无早发家族史者［(1.59±0.47) mmol/L］和对照组［(1.72±0.53) mmol/L］。

After administration of L-N〓-nitro-Arginine, a specific antagonist of NOS, it was found that the basal and stimulated NO release of blood vessels (aorta, internal carotid, renal and mensenteric artery) was decreased further. The increase of coronary blood flow stimulated by Ach was also reduced further, while the platelet aggregation degree and erythrocyte aggregation index increased significantly. Pathological examination indicated that aortic endothelium was destroyed, the blood vessels in cerebral, cardiac and renal tissues were hypertrophied further and some were almost occluded. The above fin dings resulted in SHR feeded with L-NNA having high incidence of stroke rate and high degree of renal cirrhosis when compared to control SHR. The infarct and hemorrhagic focuci in cerebral tissues, infarct focuci in cardiac tissues, atrophy and hyalinedegeneration of renal glomeruli and degeneration even necrosis of renal tubuli in renal tissues were al so found. The increase ratio of brain and heart to body weight and decrease ratio of kidney to body wei ght further demonstrated that the cerebral, cardiac and renal injuries were aggravated.

在上述结果的基础上进一步给予SHR NOS特异性拮抗剂左旋亚硝基精氨酸后，血管(胸主、颈内、肾及肠系膜动脉)NO基础释放及激动剂刺激释放进一步减少，Ach刺激冠脉流量的增加和胸主动脉内膜NOS活性也进一步减少，血小板聚集程度及红细胞聚集指数显著上升，病理切片显示胸主动脉内皮细胞破坏明显，脑心肾组织内的血管进一步增厚甚至几乎阻塞，导致饲L-NNA的SHR缺血性及出血性脑卒中发生率显著高于对照SHR，脑体重比显著升高；心肌内出现明显的缺血梗塞疤痕灶，心体重比显著升高；肾小球明显萎缩、玻璃样变性，肾小管也变性甚至坏死，使肾硬化程度显著增加呈颗粒性固缩肾，肾体重比也显著减少。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。