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Results With different concentration of sodium arsenite 0.5 ～ 5.0 μmol/L, the skin fibroblasts were markedly stimulated, and a dose effect relationship was observed.

结果与对照组比较，皮肤成纤维细胞经不同剂量的亚砷酸钠诱导后，吸光度值均有改变，存在剂量效应关系（P〈0.01）。0.5～5.0μmol／L浓度的亚砷酸钠对皮肤成纤维细胞有明显的增殖作用，而10μmol／L浓度的亚砷酸钠产生明显的毒性作用。

A new unsymmetrical Schiff base zwitterion was synthesized using L -lysine, salicylaldehyde and 2-hydroxy-l-naphthaldehyde.

L-赖氨酸与水杨醛、萘酚醛作用合成了一种不对称希夫碱配体C124H23O4N2Li。

Through researching the mechanism of biological nitrification and denitrification and actual project application of the technology and related literature materials, the obtained results are as follows:(1) Anoxybiosis is effective to the removal of COD; after the anoxic process, BOD/COD of the outflow increases, it shows that the degradation ability of wastewater is improved; anoxybiosis is insensitive when shock loading of inflow wastewater varies greatly;(2) It is demonstrated by the operation of inoculation and cultivating that biofilm takes two weeks to succeed in cultivating on packings and the removal ratios of NH3-N and COD get 50% and 70% respectively, when gas-water ratio is 6:1, HRT is 7.3 hour, water temperature is above 15°C;(3) It is demonstrated that gas-water ratio, hydraulic loading, HRT, temperature, pH etc are important factors which influence the removal effect of pollutants, when the gas-water ratio is 6:1, hydraulic loading is about 0.38kgNH3-N/m~3.d, water temperature ranges from 15°C to 27°C, pH ranges from 7.5 to 8.0, the BIOFOR removal effect of pollutants is the best;(4) The operation indicated that, this technology has strong ability of nitrification and denitrification; it has extremely vital significance for reducing the water body eutrophication;(5) It is confirmed the feasibility and the usability of preanoxic-BIOFOR process to treat L-lactic acid production wastewater. The experiment indicated that the treatment effect of this technology is very good and also shows that the technology has many advantages, such as small volume, high treatment efficiency, good effluent quality and strong endurance to load variation, this technology is a new and economical wastewater treatment process.

通过对生物脱氮机理的研究和该组合技术的实际工程应用，并结合有关文献资料，取得如下结论：(1)预缺氧池对L-乳酸生产废水中的COD去除具有一定的效果；废水中难降解的高分子物质经过缺氧工段分解为易于氧化降解的物质，浮状有机物在水解菌的作用下，将不溶性有机物水解为溶解性物质；预缺氧池具有较强的抗冲击负荷能力；(2)BIOFOR接种挂膜过程表明：HRT为7.3h、气水比为6：1、水温保持在15℃以上，经过半个月生物膜即以形成，NH_3-N和COD的去除率分别达到50％和70％左右；(3)研究表明：气水比、水力负荷、HRT、温度、pH等是影响污染物去除效率的重要因素，在气水比为6：1，NH_3-N容积负荷为0.38kgNH_3-N／m~3·d，温度在15℃-27℃，pH在7.5-8.0之间的条件下，BIOFOR对污染物的去除效率达到最佳；(4)运行表明，该组合技术具有较强的脱氮能力，对于减少水体富营养化具有十分重要的意义；(5)经过半年多实际工程的现场调试与运行，验证了利用预缺氧—BIOFOR组合工艺处理L—乳酸生产废水的可行性和实用性，得出该组合技术具有占地面积小，处理效果好，运行费用省，耐冲击负荷能力强等优点，是一项新型、经济的污水处理技术。

Results:β-L-D4A was successfully synthesized and confirmed by IR, NMR and MS. Autoradiographic bands showed similar for supernatant and intracellular HBV DNA. Episomal HBV DNA was inhibited in a dose-dependent manner. EC50 of 3 -L-D4A was 0.2 μM. The experiment of cytotoxicity gained its IC_(50) 200 μM.

结果经一系列步骤合成的化合物β-L-D4A经红外光谱、质谱和核磁共振谱鉴定正确；2.2.15细胞培养上清液病毒DNA的Southern印迹、自显影结果显示药物对病毒的抑制呈明显的浓度依赖性，计算出EC_(50)为0.2μM；细胞毒性实验显示IC_(50)为200μM。

METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity. The culture medium was renewed after 48 hours, and non-adhesive cells were removed.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

The puerperant and her relatives all signed informed consents. METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

A dual-wavelength resonance light scattering ratiometric method based on the interaction between bromothymol blue and cetylpyridinium bromide was developed for the determination of CPB.

在pH 1.5的乙酸钠-HC l缓冲溶液中，CPB本身的共振光散射很弱，BTB有一定的共振光强度，加入CPB后BTB的共振光信号显著增强，最大散射峰位于523 nm，且散射光强度与CPB的浓度呈线性关系，可以通过单波长共振光散射法检测CPB，CPB质量浓度的线性范围和检出限分别为0.05~0.60 mg/L和42μg/L。

Flow cytometry and laser confocal microscope showed a typical apoptotic peak and morphological changes of these cells. CONCLUSION: The L-amino acid oxidase from bungarus fasciatus snake venom could inhibit the HUEVC cell growth and induce the cell apoptosis.

通过两步层析法从金环蛇毒中分离得到L-氨基酸氧化酶，该氨基酸氧化酶是由单亚基组成的。L-氨基酸氧化酶能诱导HUVCE凋亡从而对其生长具有抑制作用。

Results:Taurine induced a rapid, dose-depe ndent increase in cytosolic calcium levels in suspension of neuronal cells,with the extracellular physiological Ca2+concentration.Taurine at the dose of 0.4 mmol/L exhi-bited its highest increase in cytosolic calcium(the increa se per centage of Ca2+was 12.20%±1.24%).

结果： 牛磺酸主要通过刺激细胞内钙库释放，在一定剂量范围内(0.02～6.4 mmol/L)使神经细胞［Ca2+］i轻微升高，在0.4 mmol/L时的升钙作用最大(升钙百分率为12 。20％±1.24％)。

Results:Taurine induced a rapid, dose-depe ndent increase in cytosolic calcium levels in suspension of neuronal cells,with the extracellular physiological Ca2+concentration.Taurine at the dose of 0.4 mmol/L exhi-bited its highest increase in cytosolic calcium(the increa se per centage of Ca2+was 12.20%±1.24%).

结果： 牛磺酸主要通过刺激细胞很痛内钙库释放，在一定剂量范围内(0.02～6.4 mmol/L)使原则神经细胞很痛［Ca2+］i轻微升高，在0.4 mmol/L时的升钙作用最大(升钙百分率为12 。20％±1.24％)。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。