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The results showed that the non-embryonic callus were cultured on the MS medium contained 4.0 mg · L-1 2,4-D and 0.5 mg · L-1 6-BA, embryogenic cells appeared in it. Then the single embryogenic cells formed a proembryo composed of many cells through cell division.

结果表明，非胚性愈伤组织转入到MS + 4.0 mg · L-1 2，4-D + 0.5 mg · L-1 6-BA培养基培养后，愈伤组织中先出现一些胚性细胞；然后单个胚性细胞分裂形成一个由多细胞组成的原胚。

The floatation rate of Pd (superscript 2+) reaches 100% when the concentrations of NaNO3, KBr and cetyl pyridinium bromide in solution are 0.05 g/mL, 3.0×10^(-2) mol/L, 5.0×10^(-4) mol/L(pH 4.0), respectively. Pd (superscript 2+) could be separated from Ru (superscript 3+), Al (superscript 3+), Cr (superscript 3+), Ni (superscript 2+), Ga (superscript 3+), Fe (superscript 3+) and Zn (superscript 2+) by floatation quantiatively. A method of flotation separation and enrichment of Pd (superscript 2+) was established.

当溶液中硝酸钠、溴化钾、溴化十六烷基吡啶的浓度分别为0.05 g/mL，3.0×10^(-2)mol/L，5.0×10^(-4)mol/L，在pH 4.0时，Pd(上标 2+)的浮选率达到100%，Pd(上标 2+)可与Ru(上标 3+)，Al(上标 3+)，Cr(上标 3+)，Ni(上标 2+)，Ga(上标 3+)，Fe(上标 3+)，Zn(上标 2+)定量分离，据此建立了浮选分离和富集Pd(上标 2+)的新方法。

The Ca2+ salt solution as a new regenerant was presented in this paper. The influences of the calcium salt proportioning, temperature, dosage and flow rate on anion exchange fiber regeneration were studied. High regeneration can be obtained with 0.5 mol/L CaCl2+8 g/L CaO+6.5 °Bx sucrose solution at 70～80 ℃, the dosage of the Ca2+ salt solution was about half of the raw decoloring syrup, the regenerant flow rate should be lower than that of the raw decoloring syrup. It was shown that stable decolorization rate and lower decline in exchange capacity could be achieved with the new regenerant. It performed more effectively than the traditional sodium salt regenerant with less discharge of waste water.

选用二价钙离子蔗糖溶液为再生剂，再生蔗糖脱色后的强碱性阴离子交换纤维，研究了再生液的配比、再生温度、再生液用量、再生液流速等因素对再生效果的影响，结果表明配比为0.5 mol/L CaCl2+8 g/L CaO+6.5 °Bx蔗糖的再生液在70～80℃温度下，再生剂的用量约为脱色糖汁的一半，流速应低于脱色时的流速时，再生效果优于传统的钠盐再生剂的再生效果，重复再生后的纤维脱色率能保持稳定，交换容量下降少，且再生液能回收利用，废水排放量小，是一种环保型再生液。

The Ca(superscript 2+) salt solution as a new regenerant was presented in this paper. The influences of the calcium salt proportioning, temperature, dosage and flow rate on anion exchange fiber regeneration were studied. High regeneration can be obtained with 0.5mol/L CaCl2+8g/L CaO+6.5 °Bx sucrose solution at 70~80℃, the dosage of the Ca(superscript 2+) salt solution was about half of the raw decoloring syrup, the regenerant flow rate should be lower than that of the raw decoloring syrup. It was shown that stable decolorization rate and lower decline in exchange capacity could be achieved with the new regenerant. It performed more effectively than the traditional sodium salt regenerant with less discharge of waste water.

选用二价钙离子蔗糖溶液为再生剂，再生蔗糖脱色后的强碱性阴离子交换纤维，研究了再生液的配比、再生温度、再生液用量、再生液流速等因素对再生效果的影响，结果表明配比为0.5mol/L CaCl2+8g/L CaO+6.5 °Bx蔗糖的再生液在70~80℃温度下，再生剂的用量约为脱色糖汁的一半，流速应低于脱色时的流速时，再生效果优于传统的钠盐再生剂的再生效果，重复再生后的纤维脱色率能保持稳定，交换容量下降少，且再生液能回收利用，废水排放量小，是一种环保型再生液。

Their hearts were cannulated via aorta and mounted on a Langendorff apparatus and perfused retrogradely. Single midmyocardial cells were isolated enzymatically from rabbit left free wall, I were recorded by using patch clamp techniques in the whole cell configuration before and after perfusiong of 10^(-6)mol/L and 10^(-5)mol/L ibutilide solution 10^(-6 group and 10^(-5) group.

新西兰纯种大耳白兔60只，取其心脏行Langendorff灌流，以酶解法分离出单个左心室中层心肌细胞，以10^(-6)mol/L（10^(-6)组）及10^(-5)mol/L（10^(-5)组）伊布利特外液灌流正常左室前壁中层细胞，以膜片钳技术记录I的活性，并与正常对照组进行比较。

Finally, simulation software was developed with Visual C++ and Delphi for 2D molding analysis of MIM, which affords facilities for the quality forecast of 2D thin wall parts and optimal design of mold and process parameters. In the research of molding performance of MIM feedstock, for the shortcoming of capillary rheometry, it was propounded that using spiral mold test molding performance of feedstock, and a set of spiral mold was creatively designed with quickly exchange gates and closed mold cavity. With the spiral mold, an orthogonal experiment was made on getting regress equation of flow length L〓 of the heavy alloy feedstock. Then the rule of L〓 varying with Q〓, P〓, and ΔT〓 was discovered. Q〓 ranks first in the influence on process parameters of molding performance, P〓 and Δ T〓 second. Therefore the injection rate Q〓 should be controlled primarily to improve the flow performance of feedstock.

在MIM喂料注射成形性能研究方面，针对毛细管流变仪测试法衡量流动性能的不足，提出使用螺旋线模具测试喂料熔体的注射成形性能，创造性地设计了一套可快换浇口，模腔封闭的螺旋测试模，并在螺旋测试模上采用正交实验设计，得出了高密度重合金喂料流动长度L〓的回归公式，揭示了L〓随Q〓、P〓、△T〓和S〓的变化规律，并得出工艺参数对成形性能影响的强度顺序为Q〓最大，P〓与△T〓其次，因而改善喂料流动性能应优先控制注射速率Q〓。

For landfill of Jinqianpu, if peripheral zone of yard is consisted of mild clay the longest transportation distances of CODcr and NH4+ are 56.5m and 25.75m respectively in the time limit of landfill stabilization;(5)The optimal dilution ratio of groundwater runoff volume and leachate is 7:1 to meet more over Ⅲ groundwater quality standard(GB/T14848-93);(6)To build numerical model. Using CL- as a simulation factor, the expansion with time of pollution plume was simulatively calculated when the concentration of CL- is 5000mg/L and 2000mg/L in Jinqianpu landfill by means of internationally general simulating software FEFLOW.

就金钱堡填埋场而言，若场地周围为试验亚粘土层，则在填埋场稳定化年限内和的最大迁移距离分别为56.5m和25.75m；（5）垃圾渗滤液与地下水混合稀释后地下水能够达到III类水质标准以上的混合比为地下水径流量与垃圾渗滤液入渗量之比为7：1；（6）建立污染质运移的数值模型，以为模拟因子，利用国际上通用的模拟软件FEFLOW模拟计算了金钱堡垃圾填埋场中浓度分别为5000mg/L和2000mg/L时在一系列年份对地下水污染晕的扩展。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

An ODS column was selected as the separation column at 25 ℃, and pH 4.50, 0.02 mol/L of trisodium citrate-0.05 mol/L sodium phosphate dibasic to methanol (95:5, volume ratio) without ion-pair at a flow rate of 1.0 mL/min.

该实验采用ODS柱，流动相为V(0.02 mol/L柠檬酸三钠-0.05 mol/L磷酸氢二钠)：V=95：5的溶液，用电化学检测器检测。

Results The patients with the serum potassium concentration over 4.1 mmol/L accounted for 19.4 % in the group A, 37.7 % in the group B, 94.9 % in the group C at the aorta unclamped.

结果 方法1组在主动脉开放时血清钾＞4.1mmol/L者19.4%，方法2组主动脉开放时血清钾＞4.1 mmol/L者为37.7%者，方法3组主动脉开放时血清钾＞4.1 mmol/L者94.9%。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。