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By 16S rRNA. When it was cultured in MMSW substrate, adding MWFO and SWFO as the carbon source, it could produce crude biodemulsifier of 4 g/L and 3.5 g/L respectively. Compared with biodemulsifier produced by paraffine as the carbon source, both of them could achieve higher demulsification ratio in breaking W/O and O/W model emulsion.

的一株破乳剂产生菌，在以废弃油脂MWFO、SWFO为碳源培养下，得到的生物破乳剂的粗重为4 g/L、3.5 g/L；对于W/O、O/W模型乳状液的破乳效果均可超过以液体石蜡产生的破乳剂，且以SWFO废弃油脂培养得到的生物破乳剂可以同时应用于两种模型乳状液的使用。

Experimental materials include CO2 incubator,M199, fetal calf serum,Ⅳ collagenase, 40 g/L of paraform, 20 g/L of cidex,ECGF, mouse-anti-human FⅧ monoclonal antibody(anti-FⅧ-RAg), multi-marked immune analyser(1 420, WALLAC BLCTOR2), centrifuge, inverted microscope, and immunofluorescence microscope, etc.METHODS: Three-step gradient sieve method was used.

实验材料为CO2培养箱，M199，胎牛血清，Ⅳ型胶原酶，40 g/L多聚甲醛，20 g/L戊二醛，ECGF，鼠抗人第八因子单克隆抗体，多标记免疫分析系统(1 420，WALLAC BLCTOR2)，离心机，倒置显微镜，免疫荧光显微镜等。

Cycloserine was produced using L-serine methyl ester hydrochloride as raw material. L-serine methyl ester hydrochloride was chloridized with phosphorus pentachloride to afford 2-amino-3-chloride propanic acid methyl ester hydrochloride, which was cyclized with hydroxylamine in the presence of alkali to give cycloserine.

以L-丝氨酸甲酯盐酸盐为原料制备了环丝氨酸。L-丝氨酸甲酯盐酸盐和五氯化磷在氯仿溶剂中进行氯代反应生成2-氨基-3-氯丙氨酸甲酯盐酸盐，后者和羟胺在碱性溶液中发生环合反应得到环丝氨酸。

The L/γ interface through peritectic reaction and direct participation at the L/δ interface may also play an important role.

L/γ界面通过包晶反应生长及在L/δ界面从液相中的直接生长也可能起着重要作用。

Monocytes generated from bone marrow of Balb/cj mouse were cultured for 6 days in complete RPMI 1640 medium containing 10％ FBS, rmGM-CSF and rmIL-4.50 mg·L-1 acteoside or isoacteoside was added to cells on day 6 of culture for 24 h.The surface molecules expression level of DCs and their phagocytose ability were analysis by flow cytometry.

采用细胞因子诱导法，从Balb/cj小鼠骨髓细胞贴壁分离获得单核细胞，加入含10%胎牛血清、10 μg·L-1重组小鼠粒细胞巨噬细胞集落刺激因子及重组小鼠白细胞介素-4(rmIL-4)的RPMI 1640完全培养基培养，在培养第6天，实验组加入车前子苯乙醇苷类化合物(10，50，100 mg·L-1)，对照组加入RPMI 1640或LPS(1 mg·L-1)，流式细胞仪检测DCs表面分子CD11c，CD86，MHC II和CD80的表达以及各组DCs吞噬功能的变化。

50 g/L propofol has the pharmacodynamic effect similar to that of 10 g/L propofol, but the former can delay the occurrence of serum lipid metabolism.

我们的实验是比较50 g/L异丙酚与现在临床上应用的10 g/L异丙酚在药效学上的差异及其对家兔脂代谢的影响。

50 g/L propofol has the pharmacodynamic effect similar to that of 10 g/L propofol, but the former can delay the occurrence of serum lipid metabolism.

50 g/L异丙酚与10 g/L异丙酚的药效相似，但能延缓脂代谢紊乱的发生时间。

Studied on micropropagation of Pistacia vera showed: the suitable medium that will support excellent growth of Pistacia vera was the Murashige and Skoog medium supplemented with 2. 0 mg. L��� 6-BA and 0.05mg. L��� NAA, but the frequency of shoot-tip necrosis which reached to 36. 4 percent in 35 days is high. Instead MS medium with Driver-Kuniyuki-Walnut medium, the frequency of shoot-tip necrosis were declined from 36. 4 percent to 9. 3 percent.

组织培养试验发现：以MS为基本培养基，添加2.0mg.L〓 6-BA和0.05mg.L〓NAA可增大繁殖系数和加速生长，但茎尖干枯率高，培养至35天时干枯率达36.4％；以DKW培养基为基本培养基进行增殖培养，35天时干枯率仅为9.3％，茎尖干枯率降低了27.1％。

Methods Spectrophotometry was used to study the activity of PLAP in the presence and absence of L Leu. PLAP relative activity was calculated according to the absorbance variation.

方法应用分光光度法分别测定有L－Leu和无L－Leu存在下的酶活力，根据其活力变化求出酶的相对活力；用双倒数作图法，确定L－Leu对PLAP的抑制类型并求出其抑制常数；同时也观察了pH对抑制作用的影响。

Considering the current market price of phytohormones, NAA at 1.75mg/L and 3-IBA at 4mg/L are recommended in mass cultivation of H. pluvialis .

根据4 株藻株的实验结果以及综合考虑各激素的市场价格，建议在规模化生产中，采用1.75mg/L NAA 和4mg/L 3-IBA 以提高红球藻的培养效果。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失