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Firstly, callus induction and subculture for Mikania micrantha were studied systematically. The research on callus induction showed that: Shoot tip has higher induction rate than other explants ; At 25℃ and dark regime, the callus was better cultivated on Murashige and Skoog medium (pH5.8) containing 2.0mg/L 2,4-D and 1.0mg/L KT.

首先，对微甘菊愈伤组织诱导和继代增殖培养进行系统研究，愈伤组织诱导研究表明：外植体中芽尖诱导率最高，在含2，4-D2.0mg／L、KT1.0mg／L、pH5.8的MS培养基上25℃黑暗条件下愈伤组织诱导效果较好。

And the conditions in cell suspension culture of Mikania micrantha were studied. The results showed that sucrose was the compatible carbon sucrose, and 30g/L sucrose concentration can satisfy the growth of Mikania micrantha cell; ammonium was absorbed under different sucrose concentration that haven't demonstrated significant specificity, and was completely absorbed on the lag phase and the early logarithmic phase; while nitrate was mainly absorbed on logarithmic phase. The density-dependent of Mikania micrantha cell starting to grow and density-inhibited of cell growth were proposed, the fittest inoculating quantity of Mikania micrantha in cell suspension culture was 40g/L.

并对微甘菊细胞悬浮培养条件进行研究，结果表明，微甘菊细胞生长适宜的碳源是蔗糖，并且较合适的蔗糖浓度为30g／L；在不同蔗糖浓度下氨基氮的吸收差别不大，氨基氮在迟滞期和对数期前期已基本消耗完毕，对数期生长主要利用硝基氮，因而提出，在微甘菊细胞液体悬浮培养中，氮源可以采用初始低氨基氮浓度，对数期中间维持高硝基氮，而且对数期中逐步流加少量的氨基氮；微甘菊细胞迟滞期分裂启动存在密度依赖现象，对数期生长存在密度抑制现象，最适接种量为40g／L。

PCC6803. In the enclosed photobioreactor, after 58. 5h mixotrophic cultivation, 2. 50g/L cell density, 1. 0g/L. d productivity, 15. 3μg/ml chlorophyll concentration and 25. 3%energy efficiency were achieved, which were respectively 8. 9 times, 11times, 2. 3 times and 4. 9 times as much as those in photoautotrophic culture.

在封闭式光生物反应器中，混合营养培养58.5小时，集胞藻6803藻细胞密度达到2.50g/L，是同期光自养的9倍，藻细胞产率为1.0g／L.d，是光自养培养的11倍，叶绿素浓度为15.3μg／ml，是光自养的2.3倍，能量得率为25.3％，是光自养培养过程的5倍。

Detection the early stage apoptosis cells by flow cytornetry Incubating SCL-1 cells in 6-well culture medium with 1×10~6/well, after 12～48hculture with the concentration of imiquimod 0μg/mL and 150μg/mL, gathering cellsgrowing along the wall and making monocellular suspension, washing in PBS for 2times, and then add 5μL Annexin V/FITC and 10μL PI solutions, reaction for 15min avoiding light, detection the early stage apoptosis cells by flow cytometry.

流式细胞仪测定细胞早期凋亡 SCL-1细胞以1×10~6个／孔接种于6孔培养板中，使用含0μg／mL、150μg／mL咪喹莫特的培养液培养12～48h，收集贴壁生长的细胞制成单细胞悬液，PBS洗两次，分别加入5μL Annexin V／FITC和10μL浓度20μg／mL的PI溶液，混匀后室温避光孵育15min，流式细胞仪检测，结果用Cell Quest软件分析。

METHODS: Fetal liver was obtained sterilely. Monoplast suspension was collected by collagenase digestion and mechanical separation, and then centrifuged using 1.070 g/mL Percoll separating medium and 1.077 g/mL Ficoll separating medium. Cells achieved from the interface of separatory liquids were cultured in DMEM/F12 medium, supplemented with 0.1 volume fraction of fetal bovine serum, 20 μg/L hepatocyte growth factor and 40 μg/L epidermal growth factor, for 9 days.

无菌状态下取出胎儿肝脏，以胶原酶消化法和机械分离法获得胎儿肝脏来源单细胞悬液，分别采用1.070 g/mL Percoll分离液和1.077 g/mL Ficoll分离液进行密度梯度离心，吸取界层细胞，添加含体积分数为0.1 FBS、20 μg/L肝细胞生长因子、40 μg/L表皮生长因子的DMEM/F12新鲜培养基诱导9 d。

Results: At the concentrations of 0.1, 1 and 10 μmol/L, berberine dose-dependently suppressed the formation of TRAP-positive multinucleated cells, the TRAP activity and the osteoclastic bone resorption. The strongest inhibitory effect was exhibited at the concentration of 10 μmol/L, with the inhibiting rate of 60.45%, 42.12% and 72.69% respectively.

结果：小檗碱在0.1~10μmol/L范围内，浓度依赖性地抑制TRAP阳性多核破骨细胞的形成和TRAP活性，减少破骨性骨吸收陷窝的面积；在10μmol/L浓度下，对破骨细胞的抑制作用最强，对TRAP阳性多核破骨细胞的形成和TRAP活性的抑制率分别达到了60.45%和42.12%，骨吸收陷窝面积减少72.69%。

Results: Myricetin and quercetin other than kaempferol showed the inhibitory effects against p110β PI3K, and their IC50 was 2.93μmol/L and 9.91μmol/L, respectively.

结果：杨梅素和榭槲皮素对重组人PI3K p110β催化亚基有抑制作用，IC50分别为2.93μmol/L和9.91μmol/L，而山萘黄素对PI3K p110β催化亚基则无抑制作用。

METHODS: Cultured THP-1 monocytes were induced to macrophages by 0.1 μmol/L phorbol myristate acetate, and the differentiated THP-1 macrophages were incubated with 200 μmol/L Hcy for 24, 48 and 72 hours respectively. Then cell supernatant and lysate were collected as condition medium.

在由0.1 μmol/L 佛波脂诱导分化的THP-1单核细胞中加入200 μmol/L 的高同型半胱氨酸培养24，48，72 h，将各时间点细胞上清液及裂解液作为条件培养基，加入经地塞米松及维生素D3作用6 d 后的培养瓶中继续培养24 h。

The contents of chlorophyll a in the water surface is between 7~21μg/l and the lower layer is under 5μg/l. The most dominant species of nannoplankton is Trachelomonas sp.

结果发现水库生态高度稳定，水质介於轻微到中度有机物污染，尚达不到优养化之地步，水表之叶绿素a的含量最多，但都在7~21μg/l之间，但中、下层很少不超过5μg/l。

The induced rate of rooting with naphthaleneacetic acid was higher than indolebutyric acid. The medium supplemented with 50 mg/l ascorbic acid and 0.5 mg/l NAA could improve the growth and development of root system. 57.5% of shoots could develop to the well-rooted plantlets and develop into healthy and fertile plants when planted in soil.

将芽体分割进行发根试验的结果显示，naphthaleneacetic acid诱导根系的效果优於indolebutyric acid，添加50 mg/l抗坏血酸和0.5 mg/l NAA之培养基则有更好的效果，约有57.5%的芽体可再生成具有旺盛根系的植株，经移植至土壤中也可生长健旺及正常开花。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失