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The invention provides a chemical nickel tungsten phosphorus plating solution for magnesium alloy, which consists of 10-20 g/L of nickel sulfate, 7-15 g/L of sodium tungstate, 29-50 g/L of sodium citrate, 14-30 g/L of sodium hypophosphite, 14-30 g/L of sodium carbonate, 5-12 g/L of ammonium bifluoride, additive accounting for 0.00005-0.003 percent of total weight and the balance being water.

本发明提供的是一种镁合金化学镀镍钨磷镀液。它由硫酸镍10-20g/L、钨酸钠7-15g/L、柠檬酸钠29-50g/L、次亚磷酸钠14-30g/L、碳酸钠14-30g/L、氟化氢铵5-12g/L、占总重量0.00005-0.003％的添加剂和余量的水组成。

The result showed that we have established a ITS-PCR system most suitable for Buxus sinica var. parvifolia, which came out total volume 50μL reaction system, was as follows: 2.0mmol/L Mg(superscript 2+), 0.3μmol/L primer, 0.3mmol/L dNTP, 240 ng/50μL DNA template, 1.75 U/50μL TaqDNA polymerase and the anneal temperature with 56℃.This optimized ITS-PCR system might ensure the purity and quality of ITS-PCR production. In this study, the length of the ITS fragment of Buxus sinica var.

实验结果表明，在总体积50μL的反应体系中，建立了最佳ITS-PCR扩增条件：Mg(上标 2+)浓度2.0mmol/L、引物浓度0.3μmol/L、dNTP浓度0.3mmol/L、DNA模板浓度240ng/50μL、TaqDNA聚合酶的用量1.75U/50μL和退火温度56℃，该优化体系保证了珍珠黄杨ITS-PCR产物的纯度和质量要求。

MethodsNormal human mesangial cells were divided into 4 groups: a control group(N, 5 mmol/L glucose), a high glucose group (H, 30 mmol/L glucose), a PKC inhibition group (P, 30 mmol/L glucose plus 10-5mol/L chelerythrine chloride), and an mannitol group (M, 5 mmol/L glucose plus 25 mmol/L mannitol).

将NHMC分4组： N组（对照组，5 mmol/L葡萄糖）；H组（高糖组，30 mmol/L葡萄糖）；P组（抑制剂组，30 mmol/L葡萄糖+10-5mol/L白屈菜红碱）；M组(甘露醇组，5 mmol/L葡萄糖+25 mmol/L甘露醇)。

The results of experiment are summarized as follows: The 24h-LC_(50) of Ni、 Hg and PCP to Chironomus plumosus Larva are 5.7g/L, 3.54μg/L���0.11mg/L, respectively ; The 48h-LC_(50) are 1.4g/L���2.64μg/L��� 0.09mg/L, respectively.

实验结果简述如下： Ni、Hg和五氯酚对羽摇蚊幼虫24h-LC_(50)和48h-LC_(50)分别为5.7g／L、1.4g／L；3.54mg／L、2.64mg／L和0.11mg／L、0.09mg／L。

In other three groups, the left coronary artery was ligated to establish myocardial infarction models. Following 15 minutes of coronary artery deligation, the chest was opened. Rats in the cotransplantation group were treated with 250 μL (1×1010/L) BMSCs suspension + 8 mg/L Salvianolic acid B-treated EPC suspension 250 μL (1×108/L) in five points of the myocardium surrounding the deligation region. Rats in the BMSCs group received 500 μL BMSCs suspension. Rats in the model group were injected with an equal volume of cell medium.

结扎冠状动脉15 min后再次开胸，共移植组吸取骨髓间充质干细胞悬液250 μL(浓度1×1010 L-1)+ 8 mg/L丹酚酸B预处理的内皮祖细胞悬液250 μL(浓度1×108 L-1)，于结扎区附近心肌组织分5点注射；骨髓间充质干细胞组同法注射单纯骨髓间充质干细胞悬液500 μL，模型组注射等量细胞培养液。

BMSCs were isolated from adult rats by modified bone marrow culture method. BMSCs were inoculated in basic medium containing 10%fetal bovine serum, high glucose DMEM, 100 U/L penicillin, 100 U/L streptomycin and 2 mmol/L glutamine in the control group. BMSCs were inoculated in conditioned medium (basic medium, 10 mmol/L β-glycerophosphoric sodium, 10-7 mol/L dexamethasone, 50 mg/L vitamin C).

通过改良的骨髓培养法分离成年大鼠骨髓间充质干细胞，对照组以基础培养液(10%胎牛血清+高糖DMEM+100U/L青霉素+100 U/L链霉素+2 mmol/L谷氨酰胺)培养，实验组用条件培养液(基础培养液，10 mmol/Lβ-甘油磷酸纳，10-7 mol/L地塞米松，50 mg/L维生素C)进行诱导培养。

The optimal way for inducing floral buds is to preculture the plantets on 1/2MS medium supplemented with 0.5mg/L ABA and 0.5mg/L PP333 for 35 days, then transferred them onto 1/2MS medium supplemented with 1.0mg/L PP333 and 0.1mg/L TDZ. The frequency of floral buds induction increased sharply reaching 62.2% during a period of 150 days. All the mediums were added to 30g/L sucrose and 9g/Lagar.

通过筛选，诱导金钗石斛花芽形成效果最好的培养方法是：以1／2MS附加蔗糖30g／L，琼脂9g／L，活性炭0.5g／L为基本培养基(pH5.6～5.8)，将组培苗在加有PP_(333)0.5mg／L+ABA0.5mg／L的培养基上预培养35d后，再转入到附加TDZ0.1mg／L+PP_(333)1.0mg／L的培养基中。150d内统计的花芽形成率高达62.2％。

METHODS: While hypoxia-reoxygenation groups were not given any medicine intervention as control, the treatment of xionggui diwan 1×10-6mol/L was subdivided into xionggui diwan 1 × 10-6 mol/L + non-hypoxia and xionggui diwan 1 × 10-6 mol/L + hypoxia groups, and the treatment of xionggui diwan 10 × 10-6 mol/L was subdivided into xionggui diwan 10 × 10-6 mol/L + non-hypoxia and xionggui diwan 10 × 10-6 mol/L + hypoxia groups.

缺氧-复氧前后组分为两个亚组(即缺氧-复氧前、缺氧-复氧后)，均不进行药物干预；芎归滴丸1×10-6 mol/L组分为两个亚组(即芎归滴丸1.0×10-6mol/L+不缺氧组、芎归滴丸1×10-6mol/L+缺氧组)；芎归滴丸10×10-6mol/L组分为两个亚组(即芎归滴丸10×10-6 mol/L+不缺氧组、芎归滴丸10×10-6mol/L+缺氧组)。

The results showed that the solu-bleprotein content in seedling leaves dopped dramatically with the increasing concentration（0.5mg/L���1 mg/L���1.5 mg/L���2.0 mg/L���5.0 mg/L���8.0 mg/L���of Cd2+, Cr6+, Hg2+, pb2+.

结果表明，随浓度（5mg/L、10 mg/L、15 mg/L、20 mg/L、50 mg/L、8 0 mg/L）的增加，叶绿素的含量，细胞膜脂质过氧化水平呈现先上升后下降。

Methods: A randomized, single blind controlled clinical design was used to accomplish the series clinical trials included 123 cases. The troop of gatifloxation injection with sodium chloride included 20 cases in gatifloxation group and 23 cases in ciprofloxation group; The troop of gatifloxation injection with glucose included 21 cases in gatifloxation group and 19 cases in levofloxacin group; The troop of gatifloxation pivoxil included 19 cases in gatifloxation group and 21 cases in ciprofloxation group. The antibacterial activity of clinical isolates were detected by scrips which contain gatifloxation(5ug/L), levofloxacin (5ug/L), ciprofloxation (5ug/L), spafloxation(5ug/L), cefotaxime(30ug/L), penicillin(10U/L) respectively. The MICs of sixkinds of antibiotics were detected by 2-fold agar dilution method. Result: The common data of patients in the two groups of 3 troops were similiar.

采用区组分层均衡随机单盲试验设计，分别完成甲磺酸加替沙星氯化钠注射液组试验药20例及对照药23例、甲磺酸加替沙星葡萄糖注射液组试验药21例及对照药19例、甲磺酸加替沙星片剂组试验药19例及对照药21例共123例感染患者的临床试验；用K-B法测定临床分离致病菌对加替沙星(5ug／L)、左氧氟沙星(5ug／L)、环丙沙星(5ug/L)、司帕沙星(5ug/L)、头孢噻肟(30ug／L)、青霉素(10U／L)纸片的敏感性，再用按美国国家实验室标准委员会(National Committee for Clinical Laboratory Standards，NCCLS)推荐的琼脂平皿对倍稀释法测定临床分离菌株对加替沙星、左氧氟沙星、环丙沙星、司帕沙星、头孢噻肟、青霉素的最低抑菌浓度(Minimum Inhibitory Concentration，MIC)以了解其体外抗菌活性。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。