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Results The purity and following treatment of inclusion bodies of CPB were influenced by the culturing temperature after the inducement with IPTG. The solubility of inclusion bodies of rCPB in 10mol/L urea was 2~3 times higher than in 8 mol/L urea, and the renature solution with 0.75%β-ME in 10mol/L urea could increase the solubility of inclusion bodies of rCPB, comparing with that in 10mol/L urea simply.

结果诱导后生长温度不同对rCPB包涵体的纯度及后期处理均有影响；rCPB包涵体在10 mol/L尿素溶液中的溶解度比在8 mol/L 尿素溶液中提高2~3倍；添加0.75%β-巯基乙醇能显著改善rCPB包涵体的溶解效果。

Gibberelin (GA3) with different density of 100 mg/L���200 mg/L���400 mg/L and the polyethyleneglycol with different density of 10%、15%、20%、25% were used to treat Setaria sphacelata seeds in this study.

采用100 mg/L、200 mg/L、400 mg/L赤霉素(GA3)和10%、15%、20%、25%的聚乙二醇处理非洲狗尾草种子，研究GA3和PEG对非洲狗尾草种子萌发的效应。

MS + NAA0.2 mg · L-1+6-BA2 mg · L-1 is better culture medium for shoor growth,and MS+NAA0.1 mg · L-1 is better root initiation culture medium.

栀子再生芽的生长以MS培养基加入NAA0.2mg·L~(-1)+6-BA2mg·L~(-1)为佳，而根的形成和生长以培养基MS+NAA0.1mg·L~(-1)为佳。

Maximum gallic acid yield ( 83.8%) was obtained after Escherichia coli was cultivated aerobicly in 100mL liquid culture medium with 7g/L tara tannin extract and 1g/L complementary urea on snaker (120 rpm) at initial pH 6.0 and 28℃for 28h, while maximum tannin reduction rate (43.6%) was obtained after 5g/L glucose was supplied instead of urea in the same degrading conditions above.

当塔拉单宁浸提物浓度为7 g/L、补充的尿素浓度为1 g/L、初始pH为6.0、250 mL锥形瓶装液量为100 mL、28℃振荡(120 rpm)培养28 h时，棓酸产率可达83.8%；当补充的葡萄糖浓度为5 g/L时，塔拉单宁降解率可达43.6%。

Using the young leaves of Sorbus folgneri as explants to induce callus, the best medium was 1/2MS+ 0. 5mg/L 6-BA+0.1 mg/L NAA+ 0. 5mg/L 2, 4-D, and the inducement rate could be up to 90%.

用石灰花楸的幼嫩叶片为外植体材料诱导愈伤组织，最适培养基为1／2MS+0.5mg／L 6-BA+0.1 mg／L NAA+0.5mg／L 2，4-D，诱导启动率达90％以上。

The result shows that: the extraction of petroleum ether, polysaccharide and sodium copper chlorophyllin from banana leaves perform higher spumescence ability and foam stability. The critical concentration form pastern namely CMC and the surface tension in this concentration are 1.0 g/l (34.295 mN/m), 1.0 g/l (42.398 mN/m), 3.0 g/l (40.123 mN/m) respectively, which the value is close or excess the reference samples. The emulsify capability of the extraction of petroleum ether is the best in these active ingredients, and close to the emulsify capability of Tween-80. The emulsify capability of polysaccharide and sodium copper chlorophyllin from banana leaves are follow then.

试验结果表明：香蕉叶石油醚抽出物、叶多糖和叶绿素铜钠盐具有良好的起泡性和稳泡性；其临界胶束浓度CMC值和其该浓度下的表面张力分别为：1.0g/l（34.295 mN/m），1.0g/l（42.398 mN/m），3.0g/l（40.123 mN/m），已经接近或超过参照物的表面活性；香蕉叶石油醚抽出物的乳化能力最强，乳化性能已接近乳化剂吐温-80，其次为叶多糖和叶绿素铜钠盐。

Differential Scanning Calorimetry and Small Angle X-ray Diffractometry were used to characterize the phase transitions in lamellar liquid crystal systems containing water, alkyl polysorbates, sorbitan esters, stearyl alcohol and oil. The order-disorder transition temperatures of the gelled O/W and W/O globular onion lamellar phases L(subscript O1) and L(subscript O2) and planar gelled lamellar phase L(subscript β) were measured as a function of system compositions.

采用示差扫描量热法和小角X射线衍射测试技术表征了由聚氧乙烯醚失水山梨糖醇脂肪酸酯、失水山梨糖醇脂肪酸酯、直链脂肪醇和挥发性物质组成的层状液晶体系的相转变特性，测定了水包油和油包水球形洋葱层状液晶L(下标 O1)和L(下标 O2)及平面层状液晶L的有序－无序的转变温度与体系组成之间的关系。

Female SD rats received intra-tibial injection of syngenetic Walker256 mammary gland carcinoma cells in different concertration(103/μl,104/μl or 105/μl).

方法在雌性SD大鼠左胫骨内注射Walker256细胞(103/μl、104/μl、105/μl)后1、3、5、7、10、14天观察机械痛敏阈值和CO2激光热痛敏阈值的变化，与对照组大鼠进行比较，分析癌痛组大鼠出现痛阈降低的时间。

Result Compared with the traditional shaping mode of fan type, the types of L and FI could significantly increase the leaf area and the average berry weight and the L type could significantly increase the chlorophyll content, stomatal conductance and Rubisco activity of leaves and increase the photosynthesis rate of leaves at last in the rapid growth period of berry; all the shaping types of L, FI and V could increase the average berry weight and the soluble solid content in fruit to different degree and decrease the of titrable acidity content in fruit.

结果］与传统的扇形整型方式相比，L型和FI型整型方式明显提高葡萄的叶面积和平均单果重；在果实迅速膨大期，L型还显著提高叶片的叶绿素含量、气孔导度和Rubisco活性，使叶片的光合速率显著提高；L型、FI型和V型整型方式均不同程度提高葡萄平均单果重和可溶性固形物含量，降低果实可滴定酸含量。

The optimal chitinase fermentation conditions of strain SWCH-6 were conformed by single-factor experiments and orthogonal experiments, they were colloidal chitin 25.0 g/L, tryptone 10.0 g/L, seawater 1 L, pH 8.5, 32℃, 150 r/min for 72 h.

采用单因素优化方法结合正交实验，得到菌株SWCH-6产几丁质酶的最佳发酵条件：胶体几丁质25.0 g/L，胰蛋白胨10.0 g/L，陈海水1.0 L， pH 8.5， 32℃， 150 r/min培养72 h；在该条件下酶活力达0.39 U/mL。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！