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Study on the rapid Propagation Technique of Lycoris. Herb and get follow results: In the period found of axenic clone 0.1%HgCl2 is the best disinfector to deal with the Lycoris" bulb ,as to neat part, such as root, leave, and bulbil, is fit to use 0.1%HgCl2 antisepsis 7min. And found the best effect is the bulb scale with base. Root, leaf and bulb scale without base all were not inducement adventitious buds. Different position of bulb had different culture effect. 3-15 of middle part of bulb can be induced most adventitious buds but inner and outer of least it. Incised the bulb with three types (pieces of eight, pieces of six, piece of four), and found the type of pieces of six is best to Lycoris mass production. L.sprengeri is fit MS+BA5mg/l+NAA0.1mg/l and L.squamigera is fit MS+BA5mg/l+NAA0.5mg/l, but the medium fit to culture L.longituba haven"t be found.In the period of Subculture-Found of mass production, the most multiplication of adventitious buds in MS+BA5mg/l+NAA0.5mg/l during subculture of L.sprengeri, L.squamigera.

石蒜属植物快速繁殖技术研究中，在无菌无性系建立阶段：鳞茎以0.1％HgCl_2消毒10-12分钟效果最好，而叶、根尖、鳞芽等较干净部位选用0.1％HgCl_2消毒7分钟；用三种石蒜属植物的叶片、根尖、鳞芽及带基盘与不带基盘鳞片进行培养，以带基盘鳞片诱导分化效果最理想，鳞芽易培养出芽，但数量有限，而叶片、根尖与不带基盘鳞片均未诱导分化；带基盘鳞片为石蒜属植物快速繁殖最佳外植体，以鳞茎中部3-15层芽诱导率高，较外层稍次之，内部鳞片诱导率最低；选用八等分法、六等分法、四等分法切割鳞茎，六等分法综合效果最好；每外植体带三鳞片培养最为适宜；三种石蒜属植物各自适合的培养基成分不同，换锦花在MS+BA5mg／l+NAA0.1mg／l中培养效果最好，夏水仙为MS+BA5mg／l+NAA0.5mg／l，而白花长筒石蒜在各培养基组合中培养效果均不理想，其适合的培养条件有待于进一步研究。

Two specieses, Lycoris radiate and Lycoris aurea, were selected for the studying of artificial propagation in vitro. A few specieses of Lycoris, from the southeast area of China and part of locations in Japan, were chosen to research their karyotype differentiation and to measure their genetic diversity by ISSR analysis. The results could be summarized as follows.1. The vegetative propagation conditions of Lycoris aurea and L. radiate in vitro was studied by two-scaling. Different illuminate condition had an effect on the bulblet formation rate. To the species of L. radiate, the rate was higher in the state of 16h 800-12001x illumination than in the darkness. The medium also affected the new bulblet formating rate. When the culture was MS medium 0.2 mg/L NAA 4 mg/L 6-BA, the bulblet formationg rate of L. aurea was 220%;at the same time, when the medium was MS 0.2 mg/L NAA 2 mg/L 6-BA, the rate of L.

本研究选择西南部分地区及日本的几个石蒜品种，从细胞学和DNA分子角度，分析了它们的遗传多样性水平和遗传结构状况，对红花石蒜和忽地笑的离体繁殖技术进行了初步研究，得出如下结论： 1 进行了红花石蒜和黄花石蒜双鳞片快速繁殖条件的研究，结果表明：红花石蒜在16小时800-1200 lx光照下比黑暗条件下出芽率要高；黄花石蒜在MS NAA0.2mg／L 6-BA4mg／L下出芽率为220％，红花石蒜在MS NAA0.2mg／L 6-BA2mg／L下出芽率为108％；NAA比IBA有利于石蒜生根；硅藻土显著提高黄花石蒜双鳞片出芽率，活性炭起抑制作用；6％蔗糖浓度有利于红花石蒜小鳞茎增重，MS 6-BA4mg／L NAA0.5mg／L培养基有利于小鳞茎增殖，切割一刀比两刀有利于小鳞茎增殖。

The first part consists of three experiments:(1) The rings were incubated in KH, 20, 50 mmol/L 〓 for 1 house, relaxation in response to the EDHF stimuli A23187 in 30nmol/L U46619-induced preconrtaction in the presence of 7 μ mol/L indomethacin, a cyclooxygenase inhibitor, 300μmol/L LNNA, a nitric oxide biosyhnthesis inhibitor, and 1mmol/L tetraethylammonium , a 〓 blocker, or 3 μmol/L glibenclamide , a 〓 blocker, was compared with the control;(2) After the arteries were incubated in KH, UW solution or HTK solution at 4℃ for 4 hours, endothelium-derived relaxation (percentage of 30nmol/L U46619 precontraction) was induced by A23187 in the present of 7 μmol/L indomethacir and 300μmol/L LNNA;(3) After incubation with KH, UW solution and STH (either at 37℃ in oxygenated organ chamber or at 4℃ in a refrigerator for 4 hours), endothelium-derived relaxation (percentage of 30nmol/L U46619 precontraction) was induced by A23187 in the present of 7 μ mol/L indomethacin and 300 μmol/L LNNA.

第一部分研究结果：（1）单纯浸泡于KH的冠状动脉A23187能引发66.67％的血管舒张反应，经TEA及20mmol／L、50mmol／L钾离子作用后，血管舒张反应程度显著降低，但经GBM作用后改变不明显；（2）与保存于KH的冠状动脉相比，A23187引发的血管舒张反应程度，保存于UW液的明显下降，保存于HTK液的无明显变化；（3）在37℃条件下，血管环浸泡于STH出现缓慢轻微的舒张反应，浸泡于UW液初期出现短暂收缩反应，但此后主要以舒张为主：在37℃条件下，血管经UW液保存后，U46619引发的收缩反应程度降低；不论在37℃或4℃条件下，A23187引发的血管舒张反应，经UW液保存后明显下降，但用STH保存后变化不明显。

The minimum inhibitory concentrations of 1.5% Kathan for Fusarium oxysporum f.sp. vasinfectum, F. moniliforme, Trichothecium roseum, Verticillium dahliae, and Phytophthora boehmeriae were 40, 40, 20, 20, and 2 mg/L, respectively, while EC50s were 9.20, 0.785, 0.695, 2.84, and 0.346 mg/L, respectively. MIC of 1.5% Kathan for Xanthomonas oryzae Dowson, Erwinia carotovora PV. carotovora were 1 and 10 mg/L, respectively. These results suggest that Kathan has good inhibitory activity against the 7 pathogens tested, especially against P. boehmeriae and X.

结果表明，1.5%卡松对棉花枯萎病菌的MIC和EC50分别为40mg/L和9.2034mg/L，对棉花红腐病菌的MIC和EC50分别为40mg/L和0.7845mg/L，对棉花红粉病菌的MIC和EC50分别为20mg/L和0.6951mg/L，对棉花黄萎病菌的MIC和EC50分别为20mg/L和2.8383mg/L，对棉花疫病菌的MIC和EC50分别为2mg/L和0.3459mg/L；对水稻白叶枯病菌和大白菜软腐病菌的MIC分别为1mg/L和10mg/L；说明该药剂对供试7种植物病原菌均具有良好的抑制活性，尤以对棉花疫病菌和水稻白叶枯病菌的抑制活性较强。

The apical buds and axillary buds of Dracaena cambodiana Pierre ex Gagnep. were inoculated on MS medium containing 1 mg/L BA, 0.1 mg/L NAA, 100 mg/L PVP and 30 g/L sucrose for inducing shoots. After cultured for 40~50 d, the shoots bourgeoned. They were then transferred onto MS medium supplemented with 2 mg/L BA, 0.5 mg/L KT and 30 g/L sucrose for inducing clustered buds. After 25~10 d, the clustered buds formed. The proliferation rate of the clustered buds was 300%~500% per month.

以海南龙血树的顶芽和侧芽作为外植体，把其接种于MS+BA 1 mg/L+NAA0.1 mg/L+PVP100 mg/L+蔗糖30 g/L培养基上培养40~50 d可诱导其腋芽萌发，再把萌发后所形成的新芽切割下来接种于MS+BA 2mg/L+KT 0.5 mg/L+蔗糖30 g/L的培养基上培养25~30 d可诱导形成丛生芽，丛生芽在继代培养过程中每25~30 d可增殖3~5倍。

The questing for acidic middle-temperature electroless Nickel technology leads to the preferable prescription as follows:Proportion of Nickel Sulphate and Sodium Hypophosphate 0.36; Sodium Acetate 15g/L; Stabilizer A lmg/L(Stabilizer B Img/L for continual experiment); Composite complexing agent Lactic Acid 10ml/L and acetic acid 10 ml/L; Accelerator organic acid G 6g/L; pH value 5.0; Loading Capacity ldm2/L; Temperature 70C (Temperature 75C for continual experiment); Proportion of supplement for Nickel Sulphate and Sodium Hypophosphate 1:1.2; Testing the consumed ion of Nickel solution and supplement inspissations solution every 60min by the proportion of 15% fresh solution.

最后，通过研究得出最佳工艺配方为：硫酸镍与次亚磷酸钠的摩尔比为0.36；乙酸钠15g／L：稳定剂Almg／L(周期实验加稳定剂B10mg／L)；复合络合剂组合为乳酸10ml／L+冰乙酸10ml／L：促进剂为有机酸G6g／L；pH值为5.0；装载量为1.0dm~2／L；施镀温度为70℃(周期实验为75℃)：主盐与还原剂的添加比例1：1.2；每60分钟对镀后液进行镍离子浓度的测定并按镍离子的消耗量添加浓缩液，添加比例按开缸液的15％。

The results showed that the American shad fingerlings stopped opercular movement less than half an hour after anesthetia when the fish were exposed to concentration of MS-222 at 75mg/L, clove oil at 20mg/L and bezocaine at 40mg/L. The fish recovered to normal behaviors again after exposed in air for less than 10min when transferred into fresh water. The shad fingerlings had good anaesthesia for more than 12h at 20-30mg MS-222/L, 8-10mg/L clove oil and 20-30mg/L benzocaine. The anesthetic effects were enhanced at high water temperature and changed with fish sizes. Small fish were more easily in good anaesthesia than the bigger one in MS-222 while the opposite case was found in clove oil and in benzocaine.

麻醉试验结果表明：在较高麻醉浓度（MS-222为75mg/L以上，丁香油为20mg/L以上，苯唑卡因为40mg/L以上）下，鱼很快（30min内）停止鳃盖张合运动，且停止鳃盖运动的鱼在空气中暴露一定时间（10min内）后也能够复苏；在适宜的麻醉浓度（Ms-222为20~30mg/L，丁香油为8~10mg/L；苯唑卡因为20~30mg/L）下，鱼能够进入麻醉状态，且能保持很长时间(12h)；麻醉效果随着水温的升高而增强；在20mg/L MS-222麻醉剂下，小规格鱼较大规格鱼更容易进入麻醉状态，而在10mg/L丁香油和20mg/L苯唑卡因麻醉剂下，小规格鱼却难进入麻醉状态。

After the coagulation and sedimentation in the jar test with ferric chloride, the residual COD in the supernatant was 9000 mg/L which was further oxidized by chloric acid or with Fenton method. The COD removal efficient increased with the increasing of the dosage of chloric acid from 0.5 ~ 100 g/L or the H2O2/Fe2+ from 5/2.5 ~ 50/25 g/L, and the economical dosage were suggested as chloric acid 50 g/L or the H2O2/Fe2+ 50/25 g/L which resulted in the residual COD of 400 and 3300 mg/L individually for 95 and 63 % COD removal percentages. Oxidation test showed that only 5 minutes was needed for 92 % COD removal in the case of chloric acid dosage 50 g/L.

废液A经氯化铁混凝沉淀后，COD浓度可降低至9，000mg/L，仍未符合符合工业区污水厂进厂限值(＜650mg/L)，进而使用氯酸钠直接氧化法及Fenton法以去除剩余之COD，其操作条件范围分别为氯酸钠加药量0.5g ~ 100g/L ，而最适加药量为50g/L，及Fenton法H2O2/Fe2+加药量范围为5/2.5g/L~50/25g/L，其最适H2O2/Fe2+加药量为50/25g/L，两种方法处理后残余COD浓度分别为400mg/L及3300mg/L，去除率约为95%及63%，而依此判断氯酸钠氧化处理残余COD有较好之效果，且利用氯酸钠氧化速率快，5分钟就能有92%去除率，所需水力停留时间较短，由此实验建议利用氯酸钠直接氧化处理，最适加药量为50g/L。

The forced expiratory volume in first second(FEV1) decreased 0.03 L per year, FEV1/FVC%(FEV1%) decreased 0.151%, peak expiratory flow rate 0.118 L/s, maximal mid-expiratory flow 0.04 L/s, maximal voluntary ventilation 0.876 L, vital capacity 0.04 L. Residual volume increased 0.033 L per year, functional residual capacity 0.033 L. VC,FVC,FEV1,RV,RV/TLC of healthy elderly in the exsmoker group were significantly different from nonsmokers. The declinations of lung functions were not significant in different age group of healthy elderly.

结果 用力肺活量每年下降0.032 L，第1秒用力呼气量(FEV1)每年下降0.03 L，FEV1占用力肺活量比值（FEV1%）每年下降0.151%，呼气流量峰值每年下降0.118 L/s，最大呼气中段流量每年下降0.04 L/s，最大通气量每年下降0.876 L，肺活量每年下降0.04 L，残气容积每年升高0.033 L，功能残气量每年升高0.033 L，残气容积/肺总量每年升高0.596%。

In this experiment, 250mL Erlenmeyer flask with 50mL reaction liquid(400mmol/L glucose, 10mmol/L CMP, 50mmol/L choline phosphate, 100mmol/L K 2HPO 4 KH 2PO 4, 10mmol/L MgSO 4, pH=8.0) and 1 200g/L immobilized cells were used. After 4h incubation 400mmol/L of glucose was supplemented.

结果表明：2 5 0 m L摇瓶中加入 5 0 m L反应液(葡萄糖 40 0 mmol/ L，CMP1 0 mmol/ L，磷酸胆碱 5 0 mmol/ L，K2 HPO4 -KH2 PO4 缓冲液 1 0 0 mmol/ L，Mg SO4 1 0 mmol/ L，p H=8.0 )，固定化细胞 1 2 0 0 g/ L，3 4°C、1 5 0 r/ min下，反应 4h后补加 40 0 mmol/ L葡萄糖，反应 8h可使60 %以上的 CMP转化为 CDP-胆碱。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。