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The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞，用100 TCID50柯萨奇B3病毒（CVB3）感染心肌细胞，建立病毒性心肌炎实验模型，然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞，观察心肌细胞的形态和搏动，用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白，四唑蓝比色法测定心肌细胞活性和病毒抑制率，流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

The results showed that BTH, SA, OAA and Na2SiO3 had significant induction effects, and the optimum concentration of four chemicals for resistance induction of melon against Erysiphe cucurbitacearum were 0.35, 1.0, 30 mmol/L and 10 mmol/L respectively.

各种诱导剂的最适诱导浓度分别为BTH 0.35 mmol/L、SA 1.0 mmol/L、OAA 30 mmol/L、Na2SiO3 10 mmol/L；适宜浓度下的诱导效果分别为：72.4%、49.9%、43.4%和34.9%。磷酸氢二钾(K2HPO4)在供试浓度范围内对甜瓜抗白粉病的诱导作用不显著。

Three novel rigid compounds 3, 6-bis[2-(4-pyridylethenyl]-9-ethylcarbazole (L~1), 3,6-bis[2-(2-pyridyl)ethenyl]-9-ethylcarbazole (L~2), 3,6-bis[2-(4-pyridyl)ethenyl]-9 -ethylpyridylcarbazole (L~3) were successfully synthesized by Heck reaction, which were fully characterized by elemental analyses, NMR, MS, IR spectra and single X-ray diffraction analysis.

利用Heck偶联反应，成功地合成了三种新型刚性的吡啶基咔唑类化合物：3，6-二[2-(4-吡啶基)乙烯基]-9-乙基咔唑(L~1)，3，6-二[2-(2-吡啶基)乙烯基]-9-乙基咔唑(L~2)和3，6-二[2-(4-吡啶基)吡啶基]-9-乙基吡啶基咔唑(L~3)，通过元素分析、核磁共振谱、质谱、红外光谱和单晶X-射线衍射等分析手段确证了它们的结构。

Both the HepG2 cells and L-O2 cells have the expression of RhoA protein. However, the expression level of RhoA in HepG2 is much higher than that in L-O2. we also discover that the number of HepG2 cells mults more greatly with the detection of Millicell cabin than that of L-O2 cells through the hole, and that the number of HepG2 cells mults obviously and predominantly if added to RhoA excitomotor and decreases, obviously if added to RhoA antagon.

RhoA蛋白在HepG2及L-O2两株细胞中均有表达，但肝癌细胞株HepG2的表达量远远高于正常细胞株L-O2；通过Millicell小室检测，发现HepG2细胞通过细胞小室虑孔的细胞比L-O2细胞明显增多；HepG2细胞在加入RhoA激动剂后通过细胞小室虑孔的细胞显著增多；HepG2细胞在加入RhoA拮抗剂后剂通过细胞小室虑孔的细胞显著减少。

The third passage cells had been induced by 0.1 μmol/L dexamethasome, 10 mmol/L β glycerophosphate and 50 μmol/L ascorbic acid 2 phosphate.

取第3代培养细胞用含0.1 μmol/L地塞米松、10 mmol/L β甘油磷酸钠、50 μmol/L维生素C的成骨细胞分化培养液培养。

At passage 3, fetal MSCs were induced in 100 nmol/L dexamethasone, 10 mmol/L β-glycerophosphoric acid and 50 mg/L vitamin C for 14 days.

取第3代胎儿间充质干细胞，以100 nmol/L地塞米松、10 mmol/L β-甘油磷酸和50 mg/L维生素C持续诱导14 d。

The 48 h LC50 of PHMG for Penaeus vannamei,Scophthatmus maximus and Apostichopus japonicus was 25.87 mg/L,179.91 mg/L and 6.67 g/L respectively,while Haliotis dirensicolor did not die after exposure for 48 h.

聚六亚甲基胍对南美白对虾、大菱鲆和刺参48 h LC50值分别为25.87mg/L、179.91 mg/L及6.67 mg/L；作用48 h杂色鲍无死亡。

Mixing and dilution heterotactic enthalpies of aqueous n-butanol solutions and aqueous amino acids (glycine, L-alanine, L-proline, L-valine) solutions were determined at 298.15 K by flow microcalorimetric system of 2277 Thermal Activity Monitor. The data were analyzed by using the McMillan-Mayer theory to obtain the enthalpic interaction coefficients.

利用2277热活性检测仪的流动测量系统获得了298.15K时甘氨酸、L-丙氨酸、L-缬氨酸和L-脯氨酸分别与n-丁醇在水溶液中混合过程的焓变以及各自的稀释焓，依据McMillan-Mayer理论关联得到异系焓相互作用系数，讨论了不同氨基酸与n-丁醇分子的作用机理。

When two type of oils, a model oil [ n -hexadecane ( n -C16) containing DBT] and a hydrodesulfurized diesel oil with various organic sulfur compounds, were treated with Rhodococcus erythropolis NCC-1 cells, the total sulfur content significantly decreased, from 150 to 20 mg/L for n -C16 and from 554 to 274 mg/L for diesel oil.

NCC-1能使正十六烷模拟体系中的DBT硫由150mg/L 降到 20 mg/L；使加氢柴油中的总硫由554 mg/L 降到 274 mg/L。

The white low-density powder of the the sodium salt of polyaspartic acid was got by dropping the hydrolyzable solution to wiscked high speedly carbinol which Consumption amount is about 3-6 times to the amount of solution, the powders density is about 0.35g/ml.4 Polyaspartic acid was characterized by NMR and IR.5 The scale inhibition behavior of polyaspartic acid was studied and result shows: The calcium carbonate inhibition rate is up to 99% at the dosages of 12.5mg/ Land when pH value is 9.0, Ca2+ concentration is 120mg/L and HCO3- concentration is 366mg/L.6 The primary design was put forward for industrialized production of PASP.

在此工艺下得到聚天冬氨酸钠粉术，外观为白色的轻质粉术，堆积密度0.35g／ml，不用进行脱色和粉碎。 4。用红外光谱和核磁共振谱图对聚天冬氨酸进行了表征。 5。对合成的聚天冬氨酸钠对CaCO_3的阻垢性能进行了研究。结果表明：在Ca~(2+)离子浓度120mg/L(以碳酸钙计300mg/L)，HCO_3~-离子浓度为366mg/L(以碳酸钙计600mgL)，PH值=9.0的条件下，阻垢剂浓度达到12.5mg/L时阻垢率可达99％。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！