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The waves are very useful for the determination Of these compounds, for homocystine, 6-mercaptopurine and thiouracil, the detection limits are 2×10-8, 8×10-8 and 5×10-8 mol/L,respecrespectively.

处产生的阳极化波较为灵敏，其检测限为2×10－8mol／L高胱氨酸，线性范围为8×10－8～1×10－5mol／L.6－巯基嘌呤和硫尿嘧啶在乙二胺介质中与铜产生的络合物波较好，前者在－0.83V处和后者在－0.44V处的阴极波较适合于分析。它们的检测限分别为8×10－8mol／L和5×10－8mol／L，线性范围为1×10－7～1×10－5和1×10－7～2×10－5mol／L。

Through the design of AGP-test equipments and methods which could simulate the impact of input of exogenous nutrients on eutrophication, the evolution of algae and aquatic species extinction process in Miyun Reservoir under different environmental and nutritional conditions are simulated. Then the ratio growth rate in the three simulation stages are simulated and analyzed combining the numerical analysis software MATLAB 7.0. At last, the conclusion that in the three designed stage, the total phosphorus threshold which leads to a water bloom in Miyun is respctively 0.053mg/L, 0.062mg/L, 0.064 mg/L is educed. Based on the regression relationship of total phosphorus,chlorophyll-a, total nitrogen, and the transparency,we compartmentalized the evaluation standard level of eutrophication in Miyun Reservoir.

通过设计模拟外源输入对富营养化作用的AGP试验装置和方法，模拟了不同环境条件、营养条件下密云水库优势藻种演变和富营养化过程，结合使用MATLAB 7.0数值分析软件，对3期模拟时段的比增长率进行拟合分析，得出3期设计阶段密云富营养化发生的总磷阈值为0.053mg/L、0.062 mg/L、0.064 mg/L，再根据总磷与叶绿素a、总氮、透明度之间的回归关系，划分了密云水库富营养化的评价标准级别。

The 24 h-LC50 of triphenytin to Chironomus plumosus larve,Galba pervia and Tubifex tubifex were 278.7,249.1,287.8 μg·L-1,respectively.The 7 d-KBCF of Chironomus plumosus larve to TPT at 5 and 10 μg·L-1 was 60.4,70.5,respectively.

结果表明，三苯基锡对羽摇蚊幼虫、小锥实螺、颤蚓的24 h-LC50分别为278.7、249.1、287.8μg.L-1；羽摇蚊幼虫在5、10μg.L-1TPT时的7 d-KBCF分别为60.4、70.5；小锥实螺在10、20μg.L-1TPT时的7 d-KBCF分别为2 648、1 377；颤蚓在10、20μg.L-1TPT时的7 d-KBCF分别为41.7、48.7。

N L-Leucine Producer,Strain No .145,was obtained from mutants resistant to α-amino-β-hydroxyl valeric acid and rifampin by mutagenizing Brevibacterium Tianjinese T6-13 with ul-traviolet rays,It produced L-Leucine up to 32mg/mL on shaker and 21.8mg/mL of L-Leucine was produced in 16 liter fermentor in 60 hours.

以天津短杆菌T6～13为出发菌株，采用紫外线进行诱变处理，筛选α－氨基－β－羟基戊酸和利福平抗性突变株，获得一株L－亮氨酸产生菌No.145，L－亮氨酸摇瓶产量可以达32.1mg／mL。在16L发酵罐中培养60h，L－亮氨酸产量可以达21.8mg／mL葡萄糖转化率可以达20％。

Better results could be obtained, For 40 hours fermentation, cell dry weight and PHB concentration reached 32.7 g · L-1 and 21.4g · L-1 respectively.The synthesis of Poly (β-Hydroxybutyrate-co-β-Hydroxyvalerate) by the strain G-IIIy from different precusor was studied. It was found that the strain G-IIIy could accumulate PHBV with sucrose as carbon source and propionic acid or valeric acid as precursor . In 2L self-controlled fermentor, the dry cell weight, PHBV concentration and PHBV content reached 35.8g·L-1, 22.6g·L-1 and 38.4% respectively in the case of fermentation for 42 hours and the propionic acid as precursor.

研究了添加不同前体物，G-Ⅲy菌株生产聚β-羟基丁酸和聚β-羟基戊酸共聚物的发酵条件，结果表明，此菌株能以丙酸或戊酸为前体，在蔗糖为碳源的条件下合成PHBV共聚物；在2L发酵罐中，以丙酸为前体，发酵16小时开始流加丙酸，根据发酵液pH值变化控制丙酸流加量，发酵42小时，细胞干重、PHBV产量和含量分别达到35.8g/L，22.6g/L，63.13％。

The linear concentration ranges for γ-aminobutyric acid, valine and leucine were 5.0×10^(-3)-0.12 g/L, 5.0×10^(-3)-0.08 g/L and 5.0×10^(-3)-0.12 g/L, respectively, and the detection limits (S/N=3) were 2.5×10^(-3), 2.5×10^(-3) and 2.6×10^(-3) g/L, respectively. The relative standard deviations of the peak currents of γ-aminobutyric acid, valine and leucine were 2.5%, 4.9% and 3.9%(n=7), respectively.

氨基丁酸、缬氨酸和亮氨酸的线性范围分别为5.0×10^(-3)~0.12、5.0×10^(-3)~0.08和5.0×10^(-3)~0.12 g/L，检出限(S/N=3)分别为2.5×10^(-3)、2.5×10^(-3)、2.6×10^(-3)g/L；7次平行进样峰高的相对标准偏差分别为2.5%、4.9%、3.9%。

After that, all samples were fixed with 10 g/L paraform for 45 minutes. Then 50 μL well-fixed blood was added into the polystyrene tube, meanwhile 20 μL monoclonal antibody, such as CD42a-b-c-d and PE-labeled CD42b, was respectively mixed gently with the blood sample and incubated at room temperature in dark for 30 minutes.

取50 μL固定后全血加至流式检测管，同时分别向不同管中加入20 μL糖蛋白Ib/IX/V 复合物单克隆抗体CD42a-b-c-d和PE标记的糖蛋白Ibα单克隆抗体CD42b，混匀后室温避光孵育30 min，然后CD42a-b-c-d标记的样品中加入20 μL FITC标记的羊抗鼠 IgG，混匀后室温避光孵育30 min。

This paper focused on the breeding of an L-lactic acid producing bacteria Lactobacillus easei YBQ1-1, studying the characteristics, medium components, pH and metabolic network of the mutant YBQH2-14 obtained after being selected.Using UV irradiation, L.casei YBQH2-14 was selected in the different plate media such as the medium with high concentration of glucose, lactic acid , CaCO3 -Bromocresol purple and KMnO4 -KBr.

本文主要对L-乳酸生产菌株(Lactobacillus casei YBQ1-1)进行了诱变选育，并研究了突变株YBQH2-14发酵条件对生产L-乳酸的影响，构建了L.caseiYBQH2-14的代谢网络模型，分析了分批发酵不同阶段的代谢通量，同时研究了L-乳酸的应用——黑麦麸皮粉发酵。

The structural and photocatalytic properties of TiO〓 as well as surface acidity were greatly improved by sulfation. SO〓〓/TiO〓 solid superacid with Hammett constant Ho less than -12.14 was prepared by treating the TiO〓 with 1.0mol. L��� sulfuric acid solution and then calcining at 450℃ for 3h. When compared to unmodified TiO〓, the SO〓〓/TiO〓 superacid catalyst exhibited higher anatase content, smaller crystal size, higher specific surface area, centralized pore size distribution, and the blue-shifted band edge of uv-vis adsorption spectra. Furthermore, the SO〓〓/TiO〓 superacid also showed excellent reaction activity and stability for CH〓Br photocatalytic decomposition even under the moist reaction conditions. The apparent quantum efficiency of SO〓〓/TiO〓 was 10 times of that of TiO〓 catalyst for the photocatalytic degradation of bromomethane. Therefore, the SO〓〓/TiO〓 superacid catalyst is promising for practical application in environmental remediation.It was found that both L and B acid sites exist on SO〓〓/TiO〓 photocatalyst while only L acid sites on TiO〓 photocatalyst. On the basis of experimental results, the synergistic model of superacidic centers was proposed for SO〓〓/TiO〓 photocatalyst to explain the photocatalytic behavior and the mechanism of superacidifing effects, assuming that the adjacent L and B acid sites induced by SO〓〓 comprise cooperatively the superacidic center on superacid photocatalyst. The group cooperation of the superacidic center could strengthen the surface acidity, increase surface acid sites and the adsorbtion of O〓, promote separation of photogenerated electron-hole pair, enhance the interfacial transfer of charges and reversibility of water adsorption. Consequently the excellent photocatalytic activity, reaction stability and resistibility to humidity of SO〓〓/TiO〓 superacid photocatalyst may attributed to the group cooperated effect of the superacidic center on the photocatalyst.

研究结果表明：SO〓〓与TiO〓表面的结合为螯合式双配位结构；TiO〓光催化剂表面经H〓SO〓浸渍处理后，提高了表面酸性，改善了催化剂结构和光催化性能，当H〓SO〓浸渍浓度为1.0mol.L〓、烧结温度为450℃时，可制得SO〓〓/TiO〓超强酸催化剂(H〓＜-12.14)，其结构和光催化活性得到明显改善，与TiO〓相比，SO〓〓/TiO〓光催化剂的锐钛矿含量高、晶粒小、BET比表面积大、孔径分布集中、光谱吸收边蓝移，具有优异的光催化氧化活性、稳定性及抗湿性能，光催化降解溴代甲烷的表观量子效率比TiO〓催化剂提高了一个数量级，是一种有实际应用前景的高效光催化剂；由此提出了SO〓〓/TiO〓超强酸催化剂表面集团协同作用的超强酸中心模型，认为在SO〓〓/TiO〓超强酸催化剂表面，由SO〓〓诱导的相邻L酸中心和B酸中心组成了集团协同作用的超强酸中心，其集团协同作用显著地增强了催化剂表面酸性、增大了表面酸量及O〓吸附量、促进了光生电子和空穴的分离及界面电荷转移，具有可逆吸附水的性能，是SO〓〓/TiO〓超强酸催化剂具有优异的光催化氧化活性、很好的活性稳定性及抗湿性能的重要原因。

The MTT assay was used to detect the viability of cells. Flow cytometry and laser confocal microscopy were used to identiyfy the cell cycle and apoptotic morphology after cells treated with LAO. RESULTS: An L-amino acid oxidase was successfully purified from the venom of bungarus fasciatus.

结果： 通过两步层析法从金环蛇毒中分离得到L-氨基酸氧化酶，L－氨基酸氧化酶的表观分子量通过SDS-PAGE测定约为60 kD，通过凝胶过滤测定分子量为70 kD.L－氨基酸氧化酶作用于HUVCE细胞12 h后，细胞的生长明显受到抑制，L－氨基酸氧化酶作用的半数抑制量为2.8 mg/L。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！