查询词典 Knock

The way is still long and bendy,I knock the thick wall with a key

小巷，又弯又长，我用一把钥匙，敲着厚厚的墙。。。。。

One was the pqsA' positive expression plasmid constructed by cloning the pqsA'-lacZ fusion digested from pYHP441 into miniCTX-1, whose sequence was then integrated into wild type PAO-1 chromosome by biparental mating procedure. The other was pqsA-E operon knock-out plasmid whose sequence between pqsD and pqsE operon was inserted by tetracycline cassette by the site specific insertion mutagenesis strategy and the mutant constructed by biparental mating of S17-1 that harbored the plasmid and pqsA' positive expression mutant.

一种是酶切质粒pYHP441获得pqsA'-lacZ片段后，亚克隆入质粒miniCTX-1中，构建成PqsA'的阳性表达质粒，随后将构建的质粒，通过双亲交配过程整合入野生型铜绿假单胞菌株PAO-1染色体组中；另一种是通过点特异插入诱变策略，将四环素基因盒插入启动子pqsD和pqsE之间，构建的阴性质粒转化入大肠杆菌S17-1株后，和上述pqsA'阳性表达突变株进行双亲交配过程。

I was so involved in my bood that I didn't hear you knock on the door.

23我全神惯注在看书，没有听见你在敲门。

Note①Don't puncture, knock or bure the bottle;②Keep away from heat over 50℃ and keep out of touch of children;③Please strictly comply with the Usage and Note.

注意事项：不要刺穿、摔打或焚烧储瓶；勿置于热源或高于50℃之处以及儿童可触

So ,the wolf went to the grocer for a block of calcareousness and eat it down,so ,his voice is turn to soft.then ,he came back to knock at the door of the goat,shouted loudly: open the door ,my baby goats,it's ur mom coming back~!

于是，狼跑到杂货商那里，买了一大块白垩土，吃了下去，结果嗓子变细了。然后它又回来敲山羊家的门，喊道：开门哪，我的好孩子。

In Santa Monica, All the people got Modern names like Jake or Mandy And modern bodies too In Santa Monica, on the boulevard, You'll have to dodge those in-line skaters Or they'll knock you down I never felt so lonely Never felt so out of place I never wanted something more than this But on the telephone line I am anyone, I am anything I want to be I could be a super model or Norman Mailer And you wouldn't know the difference On the telephone line I am any height, I am any age I want to be I could be a caped crusader, or space invader And you wouldn't know the difference Or would you?

在Santa Monica，在那林荫大道，你躲闪着身边的溜冰者，以免自己被撞翻在地。我从未感到如此的孤独，从未感到如此迷茫。我从未有过如此的感觉。然而在电话里我可以装扮成任何人，装扮任何我想装扮的人，我可以是一个超级名模，也可以是一个普通的诺曼第邮差，而你能察觉到其中的不同吗？在电话里我可以是任何身高，任何年纪，只要我想，我可以是一个身披斗篷的十字军战士，亦或是一个外太空的入侵者。

Acremonium. However, low frequency of integrative genetic transformation is a major obstacle to gene cloning in Cephalosporium acremonium, for example, screening and identification of genes from a DNA bank by complementation of blocked mutants, knock out or replacement of genes.

过去的一些研究表明质粒对顶头孢霉的转化率太低，限制了许多分子生物学技术的应用，如利用互补突变株从顶头孢霉的DNA文库筛选和鉴别基因、基因敲除或者基因替换。

This means the Galeb Duhr could hold a victory area on its own for some time and might even knock an opponent out of it; well worth doing on maps with shared victory areas.

这表示哥砣石人能独自守住胜利区持续一段时间，甚至将敌人推离胜利区。尤其在有共同胜利区的地图更好值得一推。

I think that there is no knock-down objection to epiphenomenalism here.

我认为没有对副现象致命一击的反对意见。

There were tears in Eppie's beautiful eyes,but she did not have time to answer Silas,as just then there was a knock on the door.

埃比美丽的眼睛里充满了泪水，可她还没来得及回答西拉斯就响起了敲门声。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。