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When OECM-1 and OC3 cells were treated with safrole 0～400 μM for 2～18 hours, NF-kB was activated at certain different levels of dosage or time points, but the level increased was not significant.

在黄樟素的实验方面，以黄樟素浓度0～400μM处理OECM-1、OC3细胞2～18小时后，OECM-1细胞中NF-kB活性虽在某些剂量或时间点有增加，但其增加之幅度都不甚明显。

However, the molecular evidence of areca in oral tumorigenesis is still not clear. In this study, the NF-kB activity in oral cells after ANE or safrole treatment was examined.

然而目前槟榔参与口腔癌化过程之分子证据仍未明了，因此本研究即是针对槟榔子水萃取液与黄樟素对於口腔上皮中NF-kB活性之调控做一探讨。

The PCR products appeared to be approximate 600 bp, the expected size. The allantoic fluid of infected embryonating eggs of 6 IBV reference strains and 6 field strains were amplified by RT-PCR using three pairs of primers of 1.7, 0.2, 0.6 kb on same and different condition, 3, 5, 9 strains of IBV were amplified respectively. While 12 different sentypes of the 12 IBV strains can be divided in 6 genotypes.

用1.7、0.2、0.6 kb 3对引物对6个IBV毒株和6个分离株的含毒尿囊液在相同和不同条件下进行RT-PCR，结果3对引物分别扩增出3、5、9株IBV，同时可将不同血清型的12个IBV株分成6种基因型。

In order to overcome the problem of multidrug resistance in human epidemic carcinomata anti-adriamycin cells (KB-A-1), the antisense and antigene oligonucleotides were used to investigate their effectiveness on inhibiting the mdr1 gene expression. The effectiveness of antisense or antigene oligonucleotide on inhibiting the multidrug resistance was detected by MTT colometric assay and ELISA.

中文题名反义与反基因寡核苷酸及其萘二酰亚胺偶联物对靶基因表达的抑制作用研究副题名外文题名 The inhibition of the targeted gene expression by the antisense or antigene oligonucleotides and their naphthylimide-conjugated derivatives 论文作者李军生导师张元兴魏东芝教授学科专业生物化工研究领域\研究方向生物化学与分子生物学学位级别博士学位授予单位华东理工大学学位授予日期2001 论文页码总数106页关键词基因表达基因治疗反义寡核苷酸反基因寡核苷酸核酸馆藏号BSLW /2001 /Q78 /264 针对人表皮癌抗阿霉素细胞株（KB-A-1）的多药抗药性问题，本文从反义核酸和反基因核酸角度，通过MTT法检测细胞生长情况，ELISA法检测基因表达产物P-gp表达水平的变化，对寡核苷酸抑制肿瘤细胞MDR1基因表达的机制进行了探讨。

The fingerprints of 10 species including 27 accessions in genus Corchorus were investigated with the technique of RAPD.Twenty-five primers were screened from 119 random primers,and a total of 329 DNA fragments were amplified ranging from 0.3～3.0 kb,253 (87.78%),which were polymorphic.

采用RAPD标记构建了黄麻属植物10个种27份材料的指纹图谱，从119个随机引物中筛选出清晰且多态性高的25个引物，共扩增出329条DNA片段，分子量在0.3～3.0 kb之间，其中294条谱带具有遗传多态性，多态比率为89.36％，平均每个引物扩增出13.16条带。

The fingerprints of 10 species including 27 accessions in genus Corchorus were investigated with the technique of RAPD. Twenty-five primers were screened from 119 random primers, and a total of 329 DNA fragments were mplified ranging from 0.3~3.0 kb, 253 (87.78%), which were polymorphic.

采用RAPD标记构建了黄麻属植物10个种27份材料的指纹图谱，从119个随机引物中筛选出清晰且多态性高的25个引物，共扩增出329条DNA片段，分子量在0.3～3.0 kb之间，其中294条谱带具有遗传多态性，多态比率为89.36％，平均每个引物扩增出13.16条带。

The mainboard intel D201GLY was interesting to us doto itsmini-ITX form and the integrated processor celeron 215, based on 65 nm mobile core yonah with 512 KB cache in the second level, and working at 1.33 GHz .

该主板英特尔d201gly很有意思，我们做它的迷你ITX的形式和综合赛扬处理器215 ，基于65纳米技术的移动核心Yonah 512 kb Cache的第二个层次，以及在1.33千兆赫。

The results showed that α-eleostearic acid showed weaker cytotoxicity than three groups of triacylglycerols for KB, Bel-7402, SGC-7901 cell lines.

结果发现α-桐酸对KB、Bel-7402和SGC-7901细胞的毒性明显弱于3组含共轭亚麻酸的甘油三酯。

The cytotoxic effects of three groups of triacylglycerols containing conjugated linolenic acid which were isolated from tung oil by NARP-HPLC on five human tumor cell lines including ECA-109, SGC-7901, Bel-7402, LS-174-T, KB, and human normal cell line L02 were investigated, and α-eleostearic acid with a purity of 96% was used as control.

以纯度为96%的α-桐酸作为对照，考察了从桐油中经NARP-HPLC分离得到的3组含共轭亚麻酸的甘油三酯对人体肿瘤细胞株ECA-109、SGC-7901、Bel-7402、LS-174-T、KB和正常细胞株L02的细胞毒性。

The 7.4 kb DNA fragment containing the S. Choleraesuis std fimbrial gene cluster was PCR amplified and cloned into the pCR-XL-TOPO vector. The open reading frames of the std fimbrial gene cluster were analyzed. The resulting recombinant DNA was transformed into Escherichia coli HB101 strain.

本实验室藉由PCR方式，增幅7.4 kb的std线毛基因组，将其选殖至pCR-XL-TOPO质体DNA，委外定序后，分析std线毛基因组之开放性读框，进而将此重组DNA送入大肠杆菌HB101，并藉由RT-PCR及西方点墨试验观察到S。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷