查询词典 KB

Methods resistance of Salmonella isolates were detected by drug susceptibility tests with KirbyBauer diffusion method. PCR was designed to detect tetA, tetB, tetC, tetD, tetE, tetG and tetK. The amplified products were sequenced and alignment analysis was performed.

用KB法测定分离株对四环素等21种抗生素的耐药情况，用PCR方法检测四环素耐药基因tetA、tetB、tetC、tetD、tetE、tetG及tetK，并对扩增产物进行测序。

Methods The resistance of Salmonella isolates were detected by drug susceptibility tests with KirbyBauer diffusion method. PCR was designed to detect tetA, tetB, tetC, tetD, tetE, tetG and tetK.

用KB法测定分离株对四环素等21种抗生素的耐药情况，用PCR方法检测四环素耐药基因tetA、tetB、tetC、tetD、tetE、tetG及tetK，并对扩增产物进行测序。

From somewhere below comes faint, ghastly tittering. VOICES drift through the cellblock, taunting

某处传来模糊的、KB的吃吃的笑声，在囚楼中漂动。

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection. A signal significantly greater than background binding was observed from the third to the fifth round of selection on CHOEGFRGFP1 and CHOK1 cell, but a part of polyclonal scFv bound EGFR specially. About 45% of the selected clones contained a fullsized insert of 1 kb. One unique human antiEGFR scFv (F4scFv) was isolated by analyzing with cell ELISA and DNA sequencing.

结果 经过5轮筛选，细胞裂解液中洗脱出噬菌体效价有500倍以上增长；细胞ELISA结果显示多克隆pscFv与CHOEGFRGFP1细胞和CHOK1细胞均有明显结合，但有部分特异性结合EGFR；菌落PCR显示约45％克隆中含有完整的1kb scFv片断；经细胞ELISA、 DNA测序检测共获得1株EGFR特异性单链抗体，命名为F4scFv。

Nuclear factor kappa B, one of the most important transcriptive factors, plays a key role in the expression of many inflammatory mediators.

而核转录因子-KB是一个重要的转录因子，对多种炎性介质的表达起着关键性的调控作用。

The genome of avian adeno-associated virus was cloned for construction of gene transfer vectors. AAAV was propagated in special pathogen-free chicken embryo using the chicken embryo lethal orphan virus as the helper virus. The double-stranded genomic DNA was extracted from precipitated AAAV viron and cloned into pCR 2.1 vector.

为了克隆禽腺联病毒(Avian adeno-associated virus， AAAV)全基因组用于构建基因转移载体研究，以鸡胚致死孤儿病毒作为辅助病毒与AAAV共接种SPF鸡胚进行AAAV的增殖，将AAAV约4.7 kb双链基因组DNA与pCR2.1载体连接，构建了含AAAV全基因组的重组质粒pAAAV并进行了测序。

Inoculating test plants through vector,observing symptom and using universal primers(R16mF2/R16mR1)for phytoplasma 16S rDNA were conducted.1.4 kb DNA fragments were amplified by polymerase chain reactionin DNA samples extracted from inoculated wheat and viruliferous Psammotettix striatus L.respectively.

采用介体接种植物，症状观察和应用植原体16S rDNA基因通用引物对R16mF2/R16mR1进行PCR扩增，在接种小麦和传毒介体中均扩增出1.4kb的特异片段，鉴定出小麦蓝矮植原体新寄主7种。

The cis-acting elements were scanned within 11.5 kb of the 3′juxtaposed region of the Yunnanese 0-thalassemia deletion using the luciferase report gene system.

利用荧光酶报告基因系统搜索了Yunnanese 0 地中海贫血缺失 3′端点下游 11 5kb区域内的调控顺序。

The excerpt in question is only 30 seconds, but zips nicely to 310 kb if you want to PM your e-mail address to me.

唯一的问题是，在除去30秒但到310千字节拉开好听如果你想你时的电子邮件地址给我。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷