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Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭质粒YCEp1为载体构建西方许旺酵母部分基因组文库，在大肠杆菌中扩增后提取混合质粒DNA，经电转化非缺陷标志酒精酵母 AS.2.1364，在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力筛选含水解淀粉的阳性转化子，从阳性转化子中分离重组质粒证实含5.0kb的插入片段，用α-淀粉酶基因两端序列设计的引物PCR扩增及扩增片段序列分析证实该片段中含有α-淀粉酶全部编码序列。

Kb, probably in the crust-mantle transition zone. The plagioclase phenocrysts are dominantly andesine with reverse zoning, and those of hornblende are Al-rich (Al2O3 ~15.9 wt%), indicative of high-pressure crystallization.

斜长石斑晶主要为中长石且具反向分带，而富铝的角闪石斑晶（Al2O3 ~15.9 wt%）也反映了高压结晶的特点。

KB Animal Magnetism — Francis Story.

469字节磁性动物-弗朗西斯故事。

The original Archimedes A305 only had 512 Kb of RAM and monochrome graphics, but its 4 MIPS ARM2 processor offered much better performance than a 68020 based Macintosh II or an 80386 based PC.

原始的阿基米德斯 A305仅有512KB内存，是用单色显示器，但是达到4MIPS的ARM2处理器却提供了比起68020的MACII或80386PC好得多的性能。

It included 4 Kb of RAM, monochrome graphics, and used an audio cassette drive for data storage. It also included a version of BASIC in ROM.

它包含4KRAM，单色图形接口，使用录音机存储，同样包含固化的BASIC。

If the base register does not contain exactly the right address, an offset of up to 4 Kbytes may be added to the base to compute the transfer address

如果基址寄存器不包含确切的地址，则可以在基址上加上不超过4 KB的偏移量来计算传送地址，即

Carl Linnaeus 'kB:l li'nI:Es

卡尔。林尼厄斯瑞典植物学家

Dot blotting assay was employed in this study to ob- serve dynamic changes of c-fos mRNA expression during reperfusion after cere- bral ischemia in rats.

本实验用改良的异硫氰酸胍一步法从脑组织中提取总RNA，与α—〓标记的c—fos cDNA（2.1 kb）探针进行斑点杂交，观察大鼠脑缺血过程中c—fos mRNA表达的变化及小檗碱对其的影响。

The deferral of build-up in Sanha condensate production during 2005 reduces Angolan output by some 10 kb/d.

推迟到2005年期间增加在桑哈的凝析油声产量，使安哥拉的产量减少了1万桶/天。

N contrast, the expression levels were low in diencephalon and brainstem in both newborn and adult mice.

kb，在第1，2周龄的小鼠翠丸中表达较高，而在这之后，其表达量急剧下降。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷