查询词典 K-1

Objective To study the relationship between K-ras mutation in pancreatic juice accompanying by serum CA 199 level and recurrence of pancreatic carcinoma.

目的 探讨胰液中K-ras 12密码子点突变联合血清CA 199检测与胰腺癌病程的关系。

Objective To investigate the diagnostic significance of detecting p16 aberrant methylation and K-ras gene mutation in pancreatic juice for pancreatic cancer.

目的 探讨胰液中K-ras基因突变和p16基因启动子区5′CpG岛甲基化联合检测在胰腺癌诊断中的价值。

Objective To evaluate the value of pancreatic juice in cytologic examination and PCR-SSP in detecting K-ras gene mutation for diagnosis of pancreatic carcinoma.

目的 评价胰液细胞学检测与聚合酶链反应―顺序特异性引物检测K-ras基因点突变诊断胰腺癌的价值。

K-ras mutation in pancreatic juice and serum CA 199 level are effective in dictators for recurrence and prognosis of pancreatic carcinoma. The analysis of muliti-gene is more valuable than that of single.

联合胰液中K-ras 12密码子点突变及血清CA 199检测可作为判断胰腺癌术后复发的有效指标，多因素分析对胰腺癌术后复发的判断更有价值。

Lysis solutions from imported and domestic genomic DNA purification kits,and 2% Trition X-100 and 5%guanidinium isothiocyanate were added into the oocysts,followed by different combining forms of freeze-thawing,proteinase K and sonication.Real-time PCR was used to determine the copies of Cryptosporidium oocyst wall protein gene.HCT-8 cells were infected by C.andersoni oocysts.Real-time PCR was used to determine the enhancement effect of fetal calf serum,glucose,ascorbic acid,folic acid,calcium pantothenate and insulin while added into RPMI-1640 medium.

在隐孢子虫卵囊悬液中分别加入进口试剂盒和国产试剂盒的裂解液以及2%Triton X-100和5%异硫氰酸胍，辅以不同组合方式使用的冻融法、蛋白酶K法和声裂法裂解卵囊，通过Real-time PCR法测定裂解产物中隐孢子虫卵囊囊壁蛋白(Cryptosporidium oocyst wall protein，COWP)基因拷贝数。

Vitamin A, carotene, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D, vitamin E, vitamin K , folacin, calcium pantothenate, niacinamide, biotin, magnesia, ferrous gluconate, zinc gluconate, Calcium Gluconate, Sodium Selenite.

维生素A，-胡萝卜素，维生素B1，维生素B2，维生素B6，维生素B12，维生素C，维生素D，维生素E，维生素k，叶酸，泛酸钙，烟酰胺，生物素，氧化镁，葡萄糖酸亚铁，葡萄糖酸钙，葡萄糖酸锌，亚硒酸钠。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

The main reasons of these phenomena can be attributed to the decrease of unit-cell free volume in lattice after the partial substitution of La〓 with K〓.

形成这一现象的主要原因可以认为是K部分替代La后，导致〓晶格内自由体积减小所致。

Author J.K. Rowling is quoted as saying "I'm delighted to be part of a publishing initiative that gives blind and partially sighted children and adults the same reading opportunities as sighted book lovers."

J.K。罗琳说"我很高兴能成为发起这项活动一分子，让盲人和弱视的孩子和大人能像正常的书虫一样有阅读的机会。"

His goal was to capture the letters in his name, but he never could find a passable D or a decent K.

他的目标是捕捉到他的名字的字母，但是他一直没能找到一个过得去的D或一个像样的K。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了