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The results are as follows: 37℃ is the most favorable temperature for induction expression of SUMO-hEGF in BL21, 5.0g/L the most best concentration of arabinose, and 4h the best time for induction expression. Meanwhile, the quantity of expression is around 20.2%. The conclusion that hEGF is the purified protein is verified by Western blotting. It is a good foundation for the development of hEGF genetic medicine.
结果表明: SUMO-hEGF在BL21中的最佳诱导表达温度为37℃、诱导剂阿拉伯糖的最佳浓度为5.0g/L、最佳诱导表达时间为4h,表达量约为20.2%;Western blot 分析证实,纯化后的蛋白是hEGF,为进一步开发hEGF基因工程药物奠定了基础。
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A few things that I liked about the book were as follows: it has a good concise treatment of Assyrian grammar.
几件事,我喜欢的书如下:它有一个良好的简明治疗亚述语法。
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Based on the results of the study, suggestions are presented as follows:(1) Some of the old people who did not think to take a health examination. It is suggested that involved organizations ought to explore the real reasons of their averseness by conducting more investigations to make these services well.
根据研究结果提出以下之建议:许多未做健康检查的老年人因为不觉得自己需要做健康检查而未做,因此建议相关单位应透过更多的调查研究或深入社区去发掘不觉得需要健康检查的真正原因,以使这些服务达到最大效用。
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This kind of conclusion is not the cook up that believe a mouth, I observed those abhor carefully Baidu even the person of bespatter Baidu, basic it is 4 kinds to be as follows
这种结论不是信口胡诌的,我仔细观察了一下那些痛恨百度甚至诋毁百度的人,基本是如下四类
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Two main aspects of the research work are as follows:(1) The paper introduces the experimental ice forces on semi-circular breakwaters which is made by State Key Laboratory of Coastal and Offshore Engineering of Dalian University of Technology and calculate the ice value for different radius semi-circular component, based on which it speculates on that horizontal ice value and water level accord with biquadratic polynomial function, meanwhile, the paper analyses incidence of ice heap to horizontal ice value of semi-circular component.
论文的主要工作有三部分:(1)主要介绍大连理工大学海岸和近海工程国家重点实验室所做的物理模拟试验,应用水平冰力计算公式计算了不同半径半圆型构件的冰力值;根据这些数值的分析,推测半圆型构件水平冰力与水位符合四次多项式函数关系;同时分析了碎冰堆对半圆型构件水平冰力影响。
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It is the problem that deserves discussing.In view of the present situation of leisure sports resources in Fujian Province, there are mainly as follows:(1) The capital input of leisure stadium is insufficient, place construction not reasonable and unbalanced as well, public facility quantity insufficient and place utilization rate not high.
从福建省休闲体育资源的现状来看,主要存在以下几个方面:(1)休闲体育场地的资金投入不足、场地建设结构不合理、各个地区场地建设发展不平衡、公共设施数量不足和场地利用率不高,被占现象仍然存在。
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It was also found that the decrease of immersion resistance and impedance of the commercial anti-corrosion of poly (vinyl chloride co-isobutyl vinyl ether) coating with increase of directly adding of nano-TiO2, which was elucidated with the theory of critical pigment volume concentration. At last, the optimal coating prescription was gotten as follows: 0.5-1.0g nano-TiO2 B, 25.6g resin, 33.4g xylene, 1.4g chlorinated paraffin, 1.5g 15% slurry of organic bentonite, 0.8g green chrome, 7.7g grey mica ferric oxide and 0.6g non-float aluminum.
直接添加纳米二氧化钛的商品化防腐涂层耐浸泡性能和交流阻抗值随添加量增加而降低,利用临界体积浓度理论等解释了该现象;通过实验获得了如下最佳涂料配方为:0.5-1.0g纳米TiO2,25.6g氯醚树脂,33.4g二甲苯,1.4g氯化石蜡52#,1.5g 15%有机膨润土浆,0.8g氧化铬绿,7.7g云母氧化铁灰,0.6g非浮型铝粉。
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There is a small action range in archery.Simple methods and analysis is inadequate,so the author,on the basis of testing the archery athletes in national team with the Qualisisy infrared photoelectric testing system,Footscan sole pressure testing system,Noraxon myoelectricity remote testing system, etc,analyzes comprehensively the action technique of the athletes in national team from the posture,body motion characteristics,body posture equilibrium and stability,distribution of centre sole pressure, myoelectricity characteristics,etc,with the methods of relative software and data statistics.The conclusions and the suggestions are as follows:Conclusion:Firstly,the communality of posture angle,muscle strength,the activation order of main muscle during raising the bow, raising the bow and the timing of the raising could be used as the evaluation index of technique action in archery;Secondly,the length of the time needed in the different periods couldn\'t be used as technique evaluation index since it has no relation with the result.But the time of raising and pulling back the row is relatively fixed,and the time has a positive relation with the archery achievement of the national team in China.Thirdly,the central index of the sole pressure could be used as the reference criterion of selecting athletes and forecasting achievement.During playing archery,the interrelation between the central index of the sole pressure and the achievement is different individually and in different periods.Fourthly,there is low stability of the archery athletes in the front and back direction during the action, caused by the athletes\' posture disequilibrium of dorsal abdominal muscle and non-professional sport shoes..Fifthly,kinematics and electromyography shows that the people on archery in China haven\'t taken the raising and pulling back the row seriously.
射箭的肢体动作幅度小,简单的手段和分析不能满足其需要,笔者结合各种运动生物力学仪器优点及射箭项目的特性,选用Qualisisy红外光点测试系统、Footscan足底压力测试系统、Noraxon肌电遥测系统等对国家队射箭队运动员进行多方位的测试,运用相关软件及数理统计方法,对运动员的射箭动作技术,从射箭动作的姿态构架、肢体运动特点,身体姿态平衡稳定性、足底压力中心分布、肌电特性等进行全面分析,结论及建议如下:结论:第一,姿态角、肌肉用力激活程度、举弓阶段主要用力肌肉激活顺序、举弓、开弓时间等的一致性可作为射箭技术动作评价指标;第二,各动作阶段所用时间的长短不能作为技术评价指标,但举、开弓的时间相对较固定,开举弓时间比与中国国家队射箭成绩正弱相关达到显著性;第三,闭眼状态下足底压力中心单位面积轨迹长与144支箭最好成绩正相关达到显著性,可作为身体素质、选材及成绩预测的参考依据;实射时身体平衡的足底压力中心指标与成绩的相关程度具有个体性差异、阶段性差异;第四,射箭运动员实射时身体在前后方向上的稳定性较差,这与运动员的站立姿态、腹背肌力量的不匹配以及射箭没有专业运动鞋有关;第五,运动学及肌电学都表明中国射箭训练对举弓、开弓阶段的重视不够,背部肌肉用力特征不明显,撒放技术合理性不高。
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The main results of this paper are given as follows: In the first chapter , the concept of fuzzy filter is introduced. Some of its properties are investigated. The structure of fuzzy filters is discussed and it is proved that the set of all fuzzy filters of a residuated lattice is a distributive and complete lattice .
本文的主要内容如下:本文的第一章在剩余格中引入了Fuzzy滤子的概念,得到了它的一些特征性质;给出了Fuzzy滤子的结构,证明了剩余格中的Fuzzy滤子之集构成完备的分配格;利用Fuzzy滤子的特有结构,在剩余格中定义了Fuzzy滤子间的两个运算"",""
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are as follows:(1)The starting reaction temperature of B/Pb 3 O 4 and B/BaCrO 4 delay compositions are independent of the proportion of the delay composition, it is about 415℃ and 780℃, But that is about 713℃ of B/CuO delay composition when B in the delay composition is in 3%~5%, and 662℃ when B is 7%~10%,(2)The reaction heat is augmenting with the augment of B in the boron series delay composition.
主要结论有:(1)B/Pb 3 O 4 和B/BaCrO 4延期药的反应起始温度分别在415℃和780℃左右,与配比没有关系;硼含量为3~5%,B/CuO延期药的反应起始温度在713℃左右,硼含量为7~10%,在662℃左右。(2)硼系延期药的反应热随硼含量的增大而增大。
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For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether
年美国个人破产法的一个改动使得破产登记急速下降,而后引起了信用卡大规模的亏损。
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Eph. 4:23 And that you be renewed in the spirit of your mind
弗四23 而在你们心思的灵里得以更新
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Lao Qiu is the Chairman of China Qiuyang Translation Group and the head master of the Confucius School. He has committed himself to the research and promotion of the classics of China.
老秋先生为中国秋阳翻译集团的董事长和孔子商学院的院长,致力于国学的研究和推广。