查询词典 In Darkness

Polarity,or action and reaction,we meet in every parto nature;in darkness and light;in heat and cold;in the ebband low o waters;in male and emale;in the inspirationand expkation o plants and animals;in the equation oquantity and quality in the luids o the anima1body;in thesystole and diastole20 the heart;in the undulations o luids-arjld--o sound:in the centriuga1and centripeta1gravity3;inelectricity,galvanism,and chemica1ainity.

两极性或作用与反作用在自然界中随处可见：黑暗与光明、炎热与寒冷、潮落与潮涨、雄性与性、动植物的吸气与呼气、动物体内的体液数量与质量的均衡、心脏的收缩与舒张、液体的波动和声音的起伏、离心力与向心力；此外在电荷、电流和化学的亲和性上也不难发现这点。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

Effect of recovery methods after thawing After thawing, the highest viability was obtained when beads of Pavlova viridis were incubated in culture medium for 48h in darkness at room temperature. As to other two algae,the suitable recovery method was to incubate beads over saturated NaCl solution in a closed jar for 12h in darkness at room temperature.

化冻后恢复方法的影响化冻后，绿色巴夫藻的胶球在培养基中室温下暗放置48h后存活率最高；另两种金藻在含有过饱和的NaCl溶液(75%RH)的密闭气相中室温下暗放置12h存活率最高。

Software Description: About Gythol Granditti : The Crypt of Darkness, Set in the town of Tor Borgh, you play as local lout Geoff Dandy, who has tried time and again to join the Town Guard--but to no avail.

Gythol Granditti ： The Crypt of Darkness 故事发生在Tor Borgh的一座城市内，你扮演Geoff Dandy的本地居民进行游戏，这些本地居民久经考验并且重新加入了Town Guard，但是仍然无济于事。

Darkness there was: at first concealed in darkness, this All was indiscriminated chaos.

只有黑暗：最初这个&一&、这个整全隐藏在黑暗中，混沌未分。

Darkness there was: at first concealed in darkness this All was indiscriminated chaos.

初始黑暗隐藏于阴暗中，一切是无法描述的混乱

Factors that influence percentage of transformation including time of preculture, dipping and coculture, type and content of antibiotic, day of delayed selection culture, intension of selection were studied. The effective transformation system of "Starkrimson" was developed as follow: Leaves precultured in darkness for three days were dipped into agrobacterium suspension for three minutes, then were cocultured in darkness on MS medium with TDZ0.5mg/L and NAA 0.3mg/L for three days, and then cultured in darkness on MS medium with TDZ0.5mg/L NAA0.3mg/L and Cef 250mg/L.

通过对叶片预培养时间、侵染时间、共培养时间、抑菌素种类和浓度、延时筛选时间、不同选择压等遗传转化影响因素的研究，建立的'新红星'苹果遗传转化体系为：叶片黑暗预培养3天，经农杆菌侵染3分钟，在含TDZ0.5mg/L，NAA0.3mg/L的MS培养基上黑暗共培养3天，然后加250mg/L Cef黑暗维持培养3天，再进行Km12.5mg/L的选择压黑暗培养至抗性愈伤出现；转为光照条件1-2次的继代筛选，获得抗性芽后，进行扩繁。

The results showed that NRA was higher in the light than in darkness;NRA increased when thalli were incubated in NO_3~——N,and it enhanced faster in the light than in darkness.

另外，对于N加富→N饥饿的处理，在光照下NO3--N加富和NH4+-N与NO3--N共同加富的NR都能在N饥饿处理后的一段时间内维持较高的活性。

In darkness, in darkness forever, to bat-flitter like a lost shrieking soul through the endless tunnels...

在黑暗中，在永远的黑暗中，蝙蝠的飞掠声，就象是个迷失的尖叫的灵魂，穿过这没有尽头的隧道

Heart of Darkness and Lord Jim (1900)are two representative works of him, and two centrally studied by researchers both at home and abroad in recent years. But the research field tends more to search the subject of works from a certain point of view.

黑暗的心脏》(Heart of Darkness，1899年)和《吉姆爷》(Lord Jim，1900年)是其主要作品中极具代表性的两部，也是国内学者近些年研究其作品最为集中的两部。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。