查询词典 IL

In contrast, IL-10 and TGF-β were continuously overproduced for the entire SARS development.

与上述细胞因子形成对比，患者IL-10以及TGF-β均在发病起即持续升高直至整个病程。

I recently had two problems that I solved by disassembling an assembly, modifying the manifest and IL and then reassembling it.

最近我有两个问题，我的拆解集会解决，修改清单和IL它，然后重新组装。&/页&

Thus, preeclampsia is associated with the absence of normal systemic skewing away from IL-17 production toward Foxp3 expression.

因此，先兆子痫者体内正常的减少产IL-17 T细胞而增加FOXP3+T细胞的系统机制缺失。

The indexes of observation are blood glucose, microdosis albumin in urine, TNF-α, IL-6 in blood serum of rats.

观察指标为血糖、尿微量白蛋白、血清TNF-α、血清IL-6。

Objective To survey the changes of expression of IFN-γ, IL-4, T-bet and GATA-3 mRNA in peripheral blood mononuclear cells in house dust mites specific immunotherapy for patients with allergic asthma.

目的 探讨在过敏性哮喘尘螨特异性免疫治疗过程中，与TH1/TH2细胞分化相关的干扰素-γ、白介素4（IL-4）、T-bet和GATA-3 mRNA 在外周血单个核细胞中表达的变化。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Meanwhile, IL-12 recombinant was transfected into human breast cancer cell line MCF-7/S.

动物实验和临床试验表明，基因重组的IL-12能够增强T细胞介导的特异性抗肿瘤免疫。

The intracellular transfection of IL-18 gene appeared to no distinct influence on biological behavior of human breast cancer cell line.

这一结果为今后基于IL-18细胞因子肿瘤免疫治疗提供了有意义的资料。

IL-6,TNF-α levels in sera were determined by means of enzyme linkage immuno-sorption assay, 3H-TdR release assay was used to measure NK cell activity, and APAAP bridge enzyme label assay was used to type subpoppulation of T lymphocytes.

用酶联免疫吸附法检测血清IL-6、TNF-α浓度，3H-TdR掺入法检测NK细胞活性，APAAP桥联酶标法进行淋巴细胞亚群分型。

B cell function .. IL-2 level and marrow cell multiplication degree of model-mouse are studied.

检测了模型鼠T、B细胞功能、IL-2水平及骨髓细胞增殖度的影响。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。