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Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.

首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I，并成功地在原核细胞E.coli进行了重组表达，筛选出稳定表达的工程菌株DH5α，摸索了稳定表达的条件，摸索了包涵体的洗涤、变性和复性条件，摸索出了复性蛋白Src I的纯化工艺，纯化过程简单，经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化，就可获得了高纯度的重组蛋白样品(纯度达99％以上)，该纯化方法适合重组蛋白的大量纯化，为Src I的性质、结构和功能研究奠定了良好的基础。

Q. On the members page it says there are 640 unreviewed images, but when I click the review tool it gives me popup window saying no image available. Why?

Q。在成员网页上标明有 640个图片没有被看过，但是当我按下&REVIEW&按钮后，提示说没有可得的图片，为什么？

A kittening box I use an under-the-bed sweater box.

一个装小猫的盒子（我用的是放在床底下的装毛衣的盒子）聊狗网q#a''2Y'-q

Ob j ect i ve To reveal the relationship between PON-1 Q/R polymorphism, serum PON-1 activity and T2DM complicated by CAD in Chinese Han ethnic group and to provide objective evidence in the prophylaxes and management of T2DM complicated by CAD.Methods The subjects of the study consist of four groups:(1) T2DM group (22 men and 14 women, mean age 56 ?8 years);(2) CAD group (35 men and 6 women, mean age 60 ? 13 years);(3) T2DM complicated by CAD group(14 men and 13 women, mean age 66 ?9 years);(4) normal-control group(21 men and 12 women, mean age 57 + 11 years).

目的与方法本研究应用聚合酶链式反应—限制性片段长度多态性方法鉴定天津地区汉族人单纯T2DM患者36例、单纯CAD患者41例、T2DM合并CAD患者27例和正常对照组33例的PON-1 Q／R基因多态性的基因型，并测定血清PON-1活性，探讨PON-1在T2DM并发CAD中的作用，为该病的防治提供客观依据。

In 1983,I left Merrill Lynch to start a consulting firm, Trading Behavior Dynamices, where I presently develop and conduct seminars on trading psychology and act in the capacity of what is commonly referred to as a trading coach.I've done countless presentations for trading companies, clearing firms, brokerage houses,banks, and investment conferences all over the world.

i/ r$ C Q 在1983年，我离开了MERRILL LYNCH 开办了一家咨询公司--交易行为有限公司，（以下专业词语太多，只能大致翻译--本人）通过公司，我不断的举办研讨会，讨论和讲授关于在交易中，心理对交易的影响的课题-这个问题是在我作为交易教练中经常被提到的问题。

Q. How do I print a long double?

Q。 怎样输出长整形数？

A: How did you do on the exam?B: Oh, you won't believe it. I got all the questions right at first, and then I decided to go back and make some changes. Guess what? I ended up getting five booboos. I'm kicking myself for that.

英语里相当于&阿Q精神&的说法有两个，一个是The grape is sour（来自伊索寓言《狐狸和葡萄》），还一个是Dutch comfort（源自英国人对北欧人的历史偏见。

The frequency synthesizer includes a completely on-chip LC VCO and a 90 degrees phase splitter for generating the I and Q LO signals to the down-conversion mixers in receive mode and up-conversion mixers in transmit mode.

频率综合者在-芯片LC VCO上-完全包含a和一产生我和 Q北美印第安人90度分相器为向挫折-转换混合者发信号示意在朝派收到方式和沿着-转换混合者在朝派传送方式。

U5 q; t; d! ^' v G7 Owww.wangfans.com Chien-Ming Wang said he was not aware of his poor career numbers against the Red Sox. But the Yankees' righthander knew that he needed to do something different.

E； S2 i y/ Q/ P3 D 王建民说，他并没有注意到他生涯对上红袜队那惨烈的成绩，不过这位洋基的右投手已经知道他需要做一些不一样的事。

Q:I've been doing playing around with Chimera Shot and it seems a bit underpowered.

Q：我最近一直在尝试奇美拉射击，它貌似有点废柴

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。