查询词典 His

This protein reduced antibody titer in mice immunized with BSA. Following the deletion of sCD152, however, there was no obvious inhibitory effect on the primary immune response triggered by the nonrelevant antigen OVA, indicating that sCD152 had an antigen-specific immunosuppressive effect. We designed a new vector, pPIC-HE-sCD152, to get entirely native sCD152 without His-tag.

为了获得既带有纯化配体表位又带有可剪切序列的可溶性sCD152蛋白，我们设计构建了含有His-tag和肠激酶识别切割序列DDDDK的sCD152表达载体pPIC-HE-sCD152，结果这一表达载体在毕赤氏酵母中同样高效分泌表达出目的蛋白，大量纯化后用肠激酶酶切去除His-tag，用以进一步功能实验，初步结果表明具有体外细胞免疫抑制功能。

Fr. Gabriele Amorth, who is the official exorcist in the Diocese of Rome, in his book "An Exorcist Tells His Story" wrote about how the truly possessed people would kneel down on their knees whenever he read out the Philippian hymn during the rite of exorcism.

罗马教区的指定「驱魔人」Gabriele Amorth神父，在他的《An Exorcist Tells His Story》一书中，论及他在驱魔过程中向受魔鬼缠身的人念斐理伯咏时，他们也会自动下跪。

His in his choice is a pronominal adjective .

His在hischoice中是代词性形容词

Resembling a pronoun, as by specifying a person, place, or thing, while functioning primarily as another part of speech. His in his choice is a pronominal adjective.

代词性的与代词相似的，如主要作为谈话的另一部分时指代人、地方或事的。his在hischoice中是代词性形容词

The number of English words with a dog are an obvious compliment, mostly used to describe and describe the bright side of things, such as ajoilydog; top-dog; water dog (good at swimming people - water ducks); Love me, love my dog; Every dog has his day mortal Jieyou his day; He worked like a dog and so on.

而英语中许多带有dog的词语都含有明显的褒义，大多用来形容和描述事物好的一面，如ajoilydog；top—dog；water dog(善于游水的人——水鸭子)；Love me，love my dog；Every dog has his day凡人皆有得意日；He worked like a dog等等。

His father died when he was young and he was put to tutelage under a cousin of his father.

His 父亲死了当他是年轻并且他被投入了对监护在他的父亲的表兄弟之下。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The manufacturer who increases the unit price of his product by his package size to lower the quantity delivered can, without undue hardship, put his product into boxes, bags, and tins that will contain even 4-iybcem, 8-ounce, one-pound, two-pound quantities of breakfast foods, cake, mixes, etc.

分析：选D项，这属词汇题型，但需要分析句子结构和上下文逻辑。该句看似复杂，但结构容易分析，主语&the manufacturer&，谓语&can put&，宾语his product， who引导定语从句，that此引导定语从句，without undue hardship介词组作插入部分。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。