查询词典 Hind.

Methods Fourteen mutated vpr fragments were selected from patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products were purified, double-cut by Hind Ⅲ and BamH Ⅰ, and the cut products were legated and transformed into competent cells JM109. The 14 reconstructed plasmids were transfected into Hela cells. Cells with pcDNA vpr-wt, pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell were established.

以14个带有HIV-1vpr基因片段的PcD-NA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vpr基因转染细胞、突变株vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经逆转录多聚酶链式反应检测目的基因转染成功后，Pi染色，用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

Methods 14 mutanted vpr fragments selected from Chines patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products purified , double-cut by Hind��� and BamH and the cut products legated and were into competent cells JM109. The 14 reconstructed plasmids electronically transfected into hela cells, and established cells with pcDNA vprwt 、pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell.

将14个带有HIV-1 vpr基因片段的pcDNA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vp r基因转染细胞、突变株 vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经RT-PCR检测目的基因转染成功后，经Pi染色用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

Native to Kenya, rock agamas can run on their hind legs, and, like the famous version, scale vertical walls.

扁头飞龙科蜥蜴原产肯尼亚，用后腿爬行，并且还能像&蜘蛛侠&一样飞檐走壁。

Hisui apologises formally as she holds his hind legs apart and examines closely.

在把他的两条后腿分开仔细检查时，翡翠道了个正式的歉。

Two B-29 style remote control barbettes and a tail gunner's station, each with paired Nudelman-Rikhter NR-23 guns were retained, including the PRS-1 Bee Hind tail gun radar.

两架B - 29式远程控制barbettes和尾炮手的车站，每个配对nudelman - rikhter正- 23枪被保留，包括公共无线电通讯服务- 1蜜蜂后肢尾炮雷达。

"But," said Albert, emitting a volume of smoke and balancing his chair on its hind legs, only madmen, or blockheads like us, ever do travel

&是啊，&阿尔贝喷出一大口烟，翘起椅子的两条腿，晃着身子说道，只有疯子或象我们这样的傻子才会出门旅行。

On April 5, 2007, qin Zhuo receives an unit to lead announcement hind to arrive automatically case, explained oneself to receive the guilty fact that Ni Mou boodles to public security mechanism according to the facts; During investigate, explained what public security mechanism has not master actively to receive the guilty fact that Shao Mou boodles according to the facts.

直至案发时，秦卓未曾支付汽车款给倪某。2006年4月至2006年8月期间，秦卓还利用职务之便，在与上海一家电器公司购销数码产品的经济往来中，又先后多次收受该公司经理邵某给予的回扣31万元，归个人所有。2007年4月5日，秦卓接单位领导通知后自动到案，如实向公安机关交代了自己收受倪某贿赂的犯罪事实；在侦查期间，主动如实交代了公安机关尚未把握的收受邵某贿赂的犯罪事实。

But, even if after again hopeful person is right 3 person at present high cost can flinch, and say from adult rate, hind 3 person be inferior to derv car, because this derv became first-rate,transfer the sources of energy.

但是，即使是再乐观的人对后三者目前高昂成本都会望而却步，而且从成熟程度上说，后三者都不如柴油车，因此柴油成了最佳的过渡能源。

Acting experienced company resembles juicer, enrol a batch of people to come in, without giving thought to,bleed white hind desertions.

代练公司就像榨汁机，招一批人进来，榨干后丢掉不管。

The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。