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Secondly, the genomic DNA is extracted from Zostera marina L. and then amplified by PCR with two oligonucleotide primers synthesized to obtain the partial nucleotide sequences of the Na~+/H~+ antiporter gene. The Na~+/H~+ antiporter gene clone was obtained after a series of manipulation: purificatioin of PCR product, ligation and transformation of recombinant plasmids, blue-white selection and so on. DNA sequence analysis shows that its DNA sequence is highly homologous with the genomic DNA sequence of E.

其次，通过提取大叶藻基因组DNA，根据设计的简并性引物进行PCR扩增，纯化PCR产物，连接，重组质粒转化，蓝白斑筛选等操作，并测序，提交测定序列到NCBI进行BLASTN，结果表明：该序列与大肠杆菌的基因组序列相似性较高，而与其它物种的Na~+/H~+逆向转运蛋白基因没有相似性。

Mechanical ventilor assist time was 4～14(average 8±2.9)h; 24h chest drainage was 110～360 (average 252±62.9)ml. All patients didn't appear thromboembolism and gas embolism and rehabilitation discharge successfully.

手术后呼吸机辅助时间4～14(8±2.9)h；24 h胸腔引流量110～360(252±62.9)ml.15例患者没有出现血栓栓塞和气体栓塞并均顺利康复出院。

The highest agglutinating activities were observed at 24 h post-injection.

溶血活力在注射疫苗后有所增强，注射后6 h时1.08×106、1.08×107cell/ml组的活力达到最高，而1.08×108cell/ml组12 h时活力达到峰值。

The present dissertation deals with:(1) Exploring the feasibility of the CO〓 methanation and the conversion CH〓/H〓 into C〓 hydrocarbons under plasma with catalyst at ambient temperature and atmosphere.(2) Designing a reactor and preparing some catalysts suited for the conversion of carbon dioxide via the cooperation effect of plasma and catalysts.(3) Optimizing reaction conditions of conversion carbon dioxide to C〓 hydrocarbons in the proposed process.(4) Studying the reaction mechanism of conversion carbon dioxide to C〓 hydrocarbons under plasma by using in situ OES technology.(5) By the results of characterizing coked catalysts, exploring the reasons that result in the coking of catalyst and the paths for decreasing the deposit in the catalyst under plasma.(6) Analyzing the energy efficiency of the plasma catalysis and exploring the paths for improving the energy efficiency.

论文的主要研究工作包括：（1）探索脉冲电晕等离子体与催化剂共同作用CO〓／H〓甲烷化和由CH〓/H〓合成C〓烃反应的可行性；（2）研制等离子体催化CO〓转化的反应器和适配的催化剂；（3）优化等离子体催化CO〓转化制C〓烃反应的工艺条件和电参数；（4）利用原子发射光谱原位技术对等离子体反应的诊断结果，对等离子体催化CO〓转化制C〓烃反应的机理进行推断；（5）通过对积碳催化剂的表征结果，分析积碳的类型及其成因，并探讨减少积碳的途径；（6）通过对等离子体催化CO〓转化制C〓烃反应能量效率的分析，探讨提高等离子体催化反应能量效率的途径。

H-bridge switching power amplifiers are popular for their high efficiency and large power output.Based on analysis and experiments of mainstream DAA and DAPS, a 10W H-bridge power amplifier circuit is designed with JAZZ 0.5um BCD technology.

本文在分析国内外数字音频功放及后级开关功率放大器现状的基础上，采用JAZZ的0.5umBCD工艺，设计了一种高效率宽功率输出范围的数字音频H桥功率放大电路。

Under the 6 h stress, SOD activity had been to maintain a high level, POD activity showed a greater change. When the stress lasted until 24 h, POD activity decreased and almost flatted compared with the control, this showed that POD activity was sensitive under the low temperature stress. Changes of CAT and SOD activity were in the line, the results indicated that activity of two enzymes coordinated the regulation of low-temperature adaptation.

本实验中，SOD活性一直保持较高的水平；POD活性表现为波动较大，当胁迫持续到24 h时，POD活性明显降低几乎与对照持平，说明POD受低温影响更敏感；CAT活性与SOD活性变化保持一致，表现出这两种酶在低温适应性调节过程中的协同作用。

Based on the analysis of the requirements of H.323-SIP interworking, this paper presents a realization scheme for H.323-SIP interworking, and discusses the key problems necessary to be solved .

本文在简要分析 H.323和 SIP 互通要求的基础上，提出了两者互通的实现方案，并对互通需要解决的关键问题进行了讨论。

Results: In HPC and Z n2+ induced groups, all the contents of MT and the activities of antioxida ses were obviously higher than those in H/R (P.05,P.01 respectively ). While using MT, the activities of SOD were progressively increased than contr ol(P.05), CAT and GSHpx activities were lower than control(P.01), bu t were significantly higher than those in I/R group(P.01). In MT antibody g roups, the activities of antioxidase were decreased extremely.

结果：HPC和锌诱导组MT含量及抗氧化酶的活力均显著高于H／R组P.05、P.01：外源MT处理后 SOD活力显著高于对照组（ P＜0.01，CAT和GSHpx活力虽然显著低于对照组但显著高于H／R组(P＜0.01)；使用M T抗体后，酶活力最低。

Results show that samples are extracted with diethyl ether for 6 h and then saponified for 3 h, when pH value is 7, the extraction rate is between 81.8％ and 88.0%, the recovery rate of the method is between 75.12％ and 84.95％, determination limit is 1.07×10-3 mg/kg.

结果显示：提取溶剂选用乙醚，提取时间6 h，皂化时间3h，在蒸馏液pH 7条件下，异辛醇萃取率为81.8％～88.0％，方法加标回收率为75.12％～84.95％，检出限为1.07×10-3 mg/kg。

The cylindrical film holes are 45° inclined and perpendicular oriented, there are totally fourteen holes placed on one ribbed wall. Geometrical parameters such as rib angle (45°, 60°, 90° and 120°), rib height (h/e=1.0, 2.0), location of the hole relative to rib on the discharge coefficients and pressure loss characteristics are all studied systematically.

实验中主要研究了肋的角度(θ=45°、60°、90°、120°)、肋的高度(h／e=1.0、2.0)、肋与气膜孔的相对位置、通道横截面的高宽比(H／W=0.5、1.0、2.0)等几何参数对气膜孔出流特性的影响，以及肋的几何结构、气膜孔的出流对通道流阻特性的影响。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。