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Methods Density gradient centrifugalization combined with adherence method were used to segregate and cultivate rat MSCs. MSCs cultivated to the 3 rd passage were characterized using flow cytometry technique. TGF-β1 with 1, 2 and 5 μg/L were pretreated on MSCs for 8 h, then serum and oxygen deprivation were used to cause analogous ischemia of MSCs in vitro. AnnexinV/PI flow cytometry technique was used to evaluate apoptosis and survival of MSCs.

采用密度梯度离心结合贴壁法分离、培养大鼠MSCs，对培养第3代的MSCs进行鉴定；用1、2、5μg/L的TGF-β1分别预处理细胞8 h，然后用血清剥夺和低氧处理建立MSCs体外模拟缺血性损伤模型，通过流式细胞术评价MSCs的凋亡及存活情况；用Western blot方法检测2μg/L的TGF-β1对Akt和p-Akt表达的影响。

He best reaction conditions of TMAA were as follows: p-benzenediol as the polymerization inhibitor and the amount 1% of acrylic acid, reaction temperature 180 ℃,reaction time 3 h, raw material mass ratio of methyl eleostearate /acrylic acid 1:0.247 and the yield of TMAA was 85%.

MAA的最佳合成条件为：反应温度180℃，反应时间3 h，桐酸甲酯和丙烯酸的质量比为1：0.247，阻聚剂对苯二酚的加入量为丙烯酸质量的1%，产率为85%。

The analytical results have shown that p ro ducts mainly consisted of some monosubstituted, disubstituted and a few trisub stituted naphthalenes. Except the low activity of HZSM-5 catalyst, all other z eolite catalysts have higher activity. HY and Hβ catalytic activities were the highest, and their naphthalene conversions attained to 79.12 and 55.79 percent respectively, but their selectivities for 2-isopropylnaphthalene, 2,6-diisopropylnaphthalene(2,6-DIPN) and 2,7-diisopropylnaphthalene ( 2,7-DIPN) were too low. ZM catalysts had good activity and much better selecti vity, especially for ZM4-3, of which its 2,6-DIPN/2,7-DIPN ratio was 2.97. Th er efore, some zeolite catalysts are recommended for shape selective isopropylat ion of coal tar naphthalene.

研究结果表明，煤焦油萘异丙基化产物主要由一取代萘、二取代萘以及少量三取代萘组成；除HZSM-5活性较低以外，其它催化剂均有较高的反应活性，其中HY和Hβ反应活性最高，萘转化率分别达75.15％和55.96％，但β位产物的选择性较差；ZM类催化剂既有一定的活性，又有较高的β选择性，是萘择形异丙基化反应理想的备选催化剂。

But after adding sodium nitroprusside (1 mmol/L),bFGF expression decreased significantly,and especially at 48 h.

而加硝普钠的心肌细胞，bFGF表达明显低于对照组，以48 h最为显著（P＜0.01）。

Comparing with the result of methyl iodide in intense laser field, some differences are observed:(1) at the same laser field intensity, the highest charged fragment ion of methyl bromide was Br(superscript 3+), lower than I(superscript 6+) of methyl iodide;(2) the dehydrogenation channel was observed in the multiphoton dissociation or Coulomb explosion of methyl bromide, but was not observed in the case of methyl iodide;(3) HBr(superscript +) was observed, but there was no similar channel in the case of methyl iodide;(4) for methyl bromide, the valid charge distance of Coulomb explosion increases with the product of p and q; while in the case of methyl iodide, the distance remained almost the same;(5) the producing channel of CH(superscript + subscript m)(m=0, 1, 2) is different from the ionization-dissociation of methyl iodide in which the stepwise dissociation of CH3(superscript +) was thought to be the main channel, CH(superscript + subscript m)(m=0, 1, 2) of methyl bromide are mainly from the products of the direct dissociation of the dehydrogenated parent ions instead of the stepwise dissociation of CH3(superscript +).

与碘甲烷在强场中的实验结果对比发现：(1)在相同的激光场强下，碘甲烷电离解离的最高价碎片离子为I(上标 6+)而溴甲烷为Br(上标 3+)；(2)溴甲烷质谱中存在母体离子的脱氢产物CHBr和CHBr(上标 2+)，而对于碘甲烷，没有检测到这些通道，C-I键首先断开；(3)质谱中存在H^79Br和H^81Br，而碘甲烷的电离解离中不存在HI产物；(4)溴甲烷库仑两体爆炸的有效电荷间距随着两碎片电荷乘积的增大而增大，而对于碘甲烷此间距几乎不随电荷乘积变化；(5)CH(m=0， 1， 2)的主要生成通道可能与碘甲烷不同，不是来自CH3的顺序脱氢，而是来自脱氢母体离子的直接解离。

The apoptosis rate increased in the experimental group with time elongating, being markedly statistically significant from 8 h compared with the control one.

实验组细胞的凋亡率随时间延长而增加，自8 h起与对照组差异有显著意义(t=15.06~20.75，P.01)。

Life as a Chelsea fan before Roman Abramovich was not very rosy but at least bearable and even though we were considered as flashy, southern softies, with largely old and overpaid European players at the end of their careers looking for a pension fund; we were not hated with such vehemence and vigour.7 h% p, H- s2 V$ F, \9 t

o2 q； Z； i l6 o# f 阿布来之前的日子对于切尔西的球迷来说也不是一段甜蜜的时光，但那是可以忍受的。尽管我们被认为是来自南边浮夸的傻子，总是使用一些昂贵而过气了的欧洲球员，至少我们没有像今天这样被如此激烈的抨击。

In pacemaker cells of SA node superfused with the oxygenated K—H solution, MDP, APA, V〓, VDD, RPF and APD〓 were—45±5mv, 48±4mv, 2. 4±0.9v/s, 89±8 mv/s, 200±11bpm and 206±15ms, respectively. Anoxia (perfused for 20min with a modified K—H solution gassed with 100% N〓 and deprived of glucose) increased MDP, APA and V〓, and decreased VDD and RPF in a timedependent manner. At anoxia for 20min, the above parameters were—58±5mv, 64±5mv, 11. 7±2. 7v/s, 24±7mv/s, 92±11bpm and 179± 14ms, respectively. ADase (10U/L).

环戊腺苷和苯基异丙基腺苷不仅能增加豚鼠窦房结起搏细胞APA、MDP和〓，且能减小VDD、RPF和〓在环戊腺苷20nmol／L浓度时，MDA、APA和〓分别增值5.4±0.7mv、7.6±0.9mv和2.4±0.5v／s，VDD、RPF和〓分别降低32±4mv／s、68±9bpm和31±12ms（p.05或0.01）。0.1μmol／L 8—苯茶碱和10μmol／L格列苯脲能明显地抑制环戊腺苷的电生理效应。

State Key Laboratory of Automotive Safety and Energy, Tsinghua University' S1 u/~3 y+ h2 H; \7 p6 h. P Seat belt is key occupant restraint equipment in vehicle.

安全带是轿车上最重要的乘员约束装置，三点紧急锁止式安全带则是轿车安全带中应用最广泛，装备数量最多的一种。

The current PoC that some of you have downloaded and have tried on your PSP Phat may include the WRONG h.bin. If it didn't work on your phat, then you probably have the wrong PoC, and need to redownload the files.

当前发布的代码包包含了一个错误的 h.bin，如果它在你的肥P上无法运行的话，你可能你拿到一个错误的代码包。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。