查询词典 Guinea

I shall be happy to receive any private suggestions of yours, as amicus curiae, but you must see the impropriety of your interfering with my conduct in this case, with such an ad captandum argument as the offer of half a guinea

我很乐于接受你作为一位&法庭之友&的私下建议；但是你用这种笼络人心的言论，像什么出半个金币，来干涉我办这案子的行动，这你应该看得出是不适当的吧。

Objective: To investigate the pathogenesis of allergic rhinitis at cell level by determing basophil histamine releasability in guinea-pigs and attempt to find an objective way for the diagnosis of allergic rhinitis.

目的：通过对变应性鼻炎豚鼠的嗜碱粒细胞释放组胺能力的测定，从细胞水平探讨变应性鼻炎的发病机制，并期望为变应性鼻炎的诊断指标提供一种客观手段。

The basophil histamine releasability is abnormal in guinea-pigs with allergic rhinitis. Enhanced histamine releasability probably plays a role in allergic rhinitis.

变应性鼻炎的豚鼠存在着嗜碱粒细胞组胺释放能力的异常，组胺释放能力的增强在变应性鼻炎的发病机制中可能起一定作用。

Biles from an animal model of incomplete stenosis of common bile duct in guinea pigs and from patients with and without gallstone were examined for the total protein concentration, protein fractionations by SDS-PAGE electrophoresis, endogenous and exogenous .beta.-glucuronidase activity, bilirubin fractionations by HPLC, ionized calcium activity, pH, cholesterol and bile salts concentrations.

我们利用天竺鼠总胆管不完全结扎的动物模式之胆汁与患有胆结石病人之胆汁，进行下列测定：总蛋白质浓度、SDS-PAGE蛋白质电泳分析、内源性与外源性葡萄糖醛酸酵素活性、胆红素高效能液态层析仪分型浓度、游离钙活性、pH值、胆固醇浓度与胆汁酸浓度。

Guinea's opposition has condemned China for canoodling with a brutish regime and denies that the deal, if it comes off, would benefit ordinary Guineans.

几内亚的反对者责备中国和一个残暴的国家同流合污，并认为就算这桩交易成功的话，对几内亚的民众也不会有好处。

Guinea's opposition has condemned China for canoodling with a brutish regime and denies that the deal, if it comes off, would benefit ordinary Guineans.

几内亚的反对派则谴责中国政府不但同一个如此血腥的政权进行合作，而且拒不认同：若取消这一项目合作，是会惠及普通的几内亚民众的。

Guinea's opposition has condemned China for canoodling with a brutish regime and denies that the deal, if it comes off, would benefit ordinary Guineans.

几内亚的反对派谴责中国和暴力派做交易，并宣称这样的交易，即使成功几内亚民众也得不到什么好处。

Guinea's opposition has condemned China for canoodling with a brutish regime and denies that the deal, if it comes off, would benefit ordinary Guineans .

几内亚的反对派则谴责中方与几内亚暴戾当局媾和，并认为即使合约生效，也不可能为几内亚的平民百姓带来任何福利。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。