查询词典 Guinea

Several physical parameters of experimental blast underpressure and morphological and function changes of auditory organs in guinea pigs expsosed to underpressure were observed and analysed in different conditions.

通过 16 4只豚鼠 318耳的观察，发现不同强度冲击波负压暴露后，中耳、内耳的形态结构和听功能发生了不同程度的创伤或损害，且具有良好的稳定性和重复性。

Blast underpressure ; auditory organ ; trauma model ; guinea pig

冲击波负压；听觉器官；创伤模型；豚鼠

Results The remarkable changes were observed in the sections of the guinea pigs exposed to experimental blast underpressure of peak more than - 45.5kPa.

结果 当冲击波负压峰值＞-45.5kPa时，耳蜗组织切片出现较明显的病理学改变，最常见的典型病变为底转鼓阶、中阶、螺旋韧带与血管纹出血，且病变程度随着负压峰值的增大而加重。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

For example, it is offering impoverished but mineral-rich Guinea US$1.5 billion for a 47% stake in the world's largest unmined reserves of iron ore.

早前中国为购买世界上最大的尚未开采的铁矿石，向几内亚提供15亿美元购买该矿47%的股份。

Objective To investigate the protective effect of basic Fibroblast Growth Factor on guinea pig utricular hair cells destroyed by Neomycin in vitro.

目的 观察碱性成纤维细胞生长因子(basic Fibroblast Growth Factor，bFGF)对新霉素所致离体椭圆囊毛细胞损害的保护作用。

bFGF protected guinea pig utricular hair cells from Neomycin injury in vitro.

新霉素对离体的椭圆囊毛细胞有较强的破坏作用；bFGF对新霉素所致离体椭圆囊毛细胞的损伤有保护作用。

Results A significantly higher number of survival utricular hair cells was seen in experimental group(P＜0.05),as compared with control group.Conclusion bFGF protected guinea pig utricular hair cells from Neomycin injury in vitro.

结果 含bFGF的实验组毛细胞的存活数目显著高于不含bFGF的实验组P结论新霉素对离体的椭圆囊毛细胞有较强的破坏作用；bFGF对新霉素所致离体椭圆囊毛细胞的损伤有保护作用。

Methods Neomycin was used to destroy guinea pig utricular hair cells in vitro.The culture in experimental groups was supplemented with bFGF.The culture in control group was not supplemented with bFGF.All utricles were observed by scanning electronic microscope,and the number of survival utricular hair cells was accounted.

采用离体的豚鼠椭圆囊培养法，对照组为普通前庭培养液培养，实验组按培养液中含和不含bFGF分组，用新霉素造成各实验组培养的椭圆囊毛细胞损伤，各组椭圆囊均行扫描电镜检查并行毛细胞计数。

Biology Any of various tropical carnivorous lizards of the family Varanidae, living in the East Indies, southern Asia, Africa, Australia, and New Guinea and ranging in length from several centimeters to 3 meters (10 feet).

巨蜥：一种蜥蜴科的热带食肉蜥蜴，生活于东印度群岛、南亚、非洲、澳洲和新几内亚。其长度从几公分到三米10英尺）不等

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。