查询词典 Gordonia

A 1.2 kb fragment of the gyrB gene of 17 Gordonia strains including type strains were determined by direct sequencing of PCR amplified fragments. Total 25 strains (8 were retrieved from public database) of genus Gordonia form a distinct phyletic line in the gyrB-based tree and are separated from other closely-related species of genera to suborder Corynebacterineae.

在Gordonia菌种间之差异研究中则发现12种Gordonia gyrB基因序列相似度介於79.3%-97.2%，16S rDNA的序列相似度为96.2%-99.7%，可知Gordonia放线菌的16S rDNA保留性极高，较难利用此基因序列进行菌种间之区分。gyrB基因之变异程度无论是在菌属，菌种甚至各分离株间均较16S rRNA基因大。

The importance of the emerging genus Gordonia in industrial and environmental biotechnology is well evidenced from recently increasing publications and patents.

Gordonia菌属是近年来逐渐受到环境污染处理的重视，且广泛被研究的一属放线菌。

Members of the metabolically diverse genus Gordonia were isolated from various biotopes including pristine and polluted sites around Taiwan.

本研究为加速环境中Gordonia菌种之筛选，利用16S rRNA基因作为标的，设计Gordonia菌属特异性引子，并配合聚合酶连锁反应之快速侦测技术鉴定此类放线菌。

Molecular detection, classification and identification of members of the genus Gordonia

Gordonia菌属放线菌之分子侦测Gordonia菌属放线菌之分子侦测，分类及鉴定

The detection and analyses of Gordonia populations within complex microbial communities were achieved precisely and rapidly by PCR-DGGE method and provide better assessment of the species diversity of Gordonia in the environment.

此外利用菌属特异性引子配合变性梯度凝胶电泳，成功应用至泡沫污水中Gordonia族群之分析，未来将探讨Gordonia在环境中之族群多样性。

This motivated us to design genus-specific oligonucleotides primer pairs which can assist in rapid detection of species of the genus Gordonia by means of PCR-specific amplification.

筛选Gordonia分离株不但可开发新放线菌资源，并且了解此类放线菌之分布及在生态中的角色，此外研究Gordonia属内菌种之亲缘关系，有助於了解各菌种之演化地位，并提供土壤中可靠且快速之鉴定结果。

The genus specificity was validated with 47 strains including wild type isolates. Preliminary screening of soil samples with Gordonia-specific primers was successful in the rapid detection of the presence of 36 Gordonia wild-type isolates.

经多序列分析比对软体计算并比较gyrB基因序列，可知Gordonia菌属与7种邻近放线菌的gyrB基因序列相似度介於72.8-82.1%，而在16S rDNA序列相似度则介於90.0-95.4%，gyrB基因可明显区分Gordonia菌属与其他属之放线菌。

Phylogenetic analysis based on gyrB gene is consistent with those of 16S rDNA tree and provided a better discrimination within the species of Gordonia compared to 16S rRNA gene. The present study demonstrates that gyrB gene analysis will aid in describing novel species belonging to the genus Gordonia.

本研究利用上述两基因序列相似度初步判定4株本土Gordonia新菌种之分类地位，其中分离株CC-AB07经由生理生化特性及化学组成分析，已确认为本土Gordonia新菌种。

Sequence similarity of gyrB sequence from twelve Gordonia type strains ranged from 79.3% to 97.2%, corresponding to 270-41 nucleotide differences while there was only 0.3-3.8% difference in 16S rRNA gene sequence similarity at the interspecies level.

本研究之结果将可利用gyrB基因作为Gordonia菌种快速鉴定之依据，若两菌株之16S rDNA序列相似度大於99.7%，gyrB基因相似度大於97.2%，则判定两菌株为相同菌种。

The Gordonia specific 16S rDNA fragment (829 bp or 436 bp) was successfully amplified for all the reference Gordonia species with the designed primer pair G268F/G1096R or G699F/G1134R whereas, no amplification was noted for closely-related species from other genera.

研究结果显示，利用G268F/G1096R及G699F/G1134R两组Gordonia菌属特异性引子，可有效区别Gordonia菌属与其他放线菌菌属，并且利用此侦测技术已从台湾分离出36株Gordonia分离株。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。