查询词典 Germinal

DNA fluorescence dye Hoechest 33342 was used tomonitor the condensation of chromatin during germinal vesicle breakdown.

采用直接取核法获取卵母细胞生发泡，用同步化的HeLa细胞裂解液构建非细胞体系，将游离的生发泡培养在裂解液中，并加入荧光染料Hoechest 33342，观察染色质的变化情况。

Germinal vesicle transfer is a technique developed in recent years, which may be a valuable research procedure to study cell cycle regulation and characterize the cytoplasmic-nuclear interplay during maturation and fertilization of oocytes.

通过GV移植可以研究细胞周期调控、成熟及受精时细胞核与细胞质之间的相互作用。

In one group oocytes were cultured for 3h and in the other one for 6h. Incidence of germinal vesicle breakdown was observed following the culture with BL-Ⅰ.

BL-Ⅰ培养液培养结束时，观察卵母细胞生发泡破裂的发生率，再用不含BL-Ⅰ的培养液继续培养，进一步观察其成熟动力学的变化，即卵母细胞GVBD过程和第一极体形成过程。

The results showed that chromium trichloride could inhibit the extruding of the first polar body and affect the quality and the viability of mouse oocyte and reduce the rate of IVF and the number of oocytes of superovulation, inhibit the germinal vesicle breakdown of mouse oocytes in vitro culture, but little impact on GVBD of mouse oocytes in vivo .

结果表明，三氯化铬可以抑制卵母细胞第一极体的释放，降低小鼠超排卵数和卵母细胞的存活率和体外受精率。

With immunofluorescencecytochemistry and confocal microscopy,we found there were a lot of MAPK from 0-hour GV oocytes to 44-hour MII oocytes,and no active MAPK in 0-hour GVoocytes and no MAPK in the germinal vesicle.

通过体外培养猪卵巢卵母细胞至完全成熟，在培养一定时间取样用免疫荧光化学与激光共聚焦显微术结合的方法观察MAPK和p90rsk在卵内的分布规律，研究了猪卵母细胞中MAPK和p90rsk的表达和定位。

Interaction between MAP kinase and MPF influerlces meiotic maturation and fertilization of oocyte throughout the animal kingdom, including stimulation of germinal vesicle breakdown, suppression of DNA replication, control of meiotic chromosome segregation, maintenance of metaphase Ⅱ arrest, and resumption and completion of second meiosis.

方法分别用MAPK激酶特异性抑制剂（U0126）和MPF特异性抑制剂抑制受精卵细胞MAPK和MPF的活性，通过同位素测定MAPK和MPF的活性变化，利用显微镜进行形态观察来判定MAPK活性变化对受精卵发育的影响。

Inseminated in 8-9 hrs after GVBD, the ova have the highest hatching rate (more than 86% in the concerned spawn).Based on the above insemination research of the oocyte maturation in vitro, recombinant opAFP-pUC19 was microinjected into the germinal vesicle of oocyte of maturation in vitro.

经过卵母细胞时期在生发泡中注射外源抗冻蛋白基因的体外成熟卵，其受精率（70～80%）和孵化率（38～75%）均低于对照组10～30%，在进行外源抗冻蛋白基因注射后授精孵出的小鱼长至半成体和成体鱼后，分别用斑点杂交和Southern blo-tting分析外源基因的整合。

Japonicus.Nerve extraction substance of sea star and dithiothreitol has been reported to be effectively to induce oocyte maturation in sea cucumbers. In this study we tested their effects on oocyte maturation induction in sea cucumber A. japonicus. The results are as follows, oocyte of A. japonicus does not get mature spontaneously. Neither NES alone nor NES with follicle suspension can induce oocyte maturation. While DTT can prominently increase the percentages of germinal vesicle break down. When the concentration of DTT is between10-1 mol/L and 10-3 mol/L, the effect of maturation induction is more sufficient. When the concentration is about 10-2 mol/L, the percentage of GVBD can reach up to more than 90%.

分别利用在海参中有诱导作用的海星神经提取液和二硫苏糖醇对从成熟卵巢解剖出的刺参卵母细胞进行诱导成熟试验，结果显示，刺参的卵母细胞在海水中不会发生自发成熟，海星神经提取液以及神经提取液加滤泡悬浮液对卵母细胞没有诱导成熟的作用，而二硫苏糖醇处理可以诱导卵母细胞生发泡破裂，当DTT溶液的浓度处于10-1 mol/L到10-3 mol/L之间时，对刺参卵母细胞的促熟作用较为明显，在10-2 mol/L左右时，卵母细胞的诱导成熟率可以达到90%以上。

RESULTS: The rates of germinal vesicle breakdown were 98%, 22.2%, 40.1% and the rates of the first polar body (PB1) extrusion were 77.7%, 15.5%, 27.6% for M16, Hx and FA groups, respectively. The rates of GVBD and PB1 were all increased with the addition of FA compared with the Hx groups and comparison was significant (P.05). Hx and FA did not influence chromosome, but FA reversed the size of metaphase spindle elongated by Hx.

结果：M16培养液组、Hx模型组和FA处理组的GVBD率分别为98%、22.2%、40.1%，其PB1排出率分别为77.7%、15.5%、27.6%，各组间的差异均具有统计学意义(P.05)；次黄嘌呤和叶酸对染色体的形态没有影响，而次黄嘌呤使正常的纺锤体变长、变宽、面积变大，FA组较Hx模型组增大的纺锤体有所恢复，并接近于正常水平。

The cumulus cells of OCC were cut off and dispersed by 1 mL syringe. The cumulus cells were co-cultured with the immature oocytes retrieved from the COH cycles after they adherent to the bottom of the dish. The immature oocytes were experienced IVM procedures in different culture media. They were divided into 3 groups(the oocytes at germinal vesicle stage from one woman were allotted to the same group randomly). Group 1: basic culture medium+ human follicular fluid; Group 2: solution A+ cumulus cells; Group 3: solution A+ OCC+ follicle stimulating hormone + epidermal growth factor. Then, the maturation rate, fertilization rate and formation rate of available embryo were observed.

在控制性促排卵周期有未成熟卵母细胞时，将同周期成熟卵丘复合体切出部分卵丘细胞，用1 mL注射器抽打分散细胞，贴壁培养。113个治疗周期中，298枚生发泡期卵母细胞经3种不同培养液体外成熟培养(同一病人的生发泡期卵被随机分到某同一组中)：第1组28个周期中73枚：基础培养液+卵泡液；第2组40个周期中115枚：A液+分散贴壁的卵丘细胞；第3组45个周期中110枚：A液+分散贴壁的卵丘细胞+促卵泡生成激素+表皮生长因子。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。